{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:42:04Z","timestamp":1785408124081,"version":"3.56.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T00:00:00Z","timestamp":1775520000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T00:00:00Z","timestamp":1775520000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,4,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Proteus mirabilis<\/jats:italic>\n                      is increasingly recognized as a carrier of carbapenemases, which are particularly difficult to identify, since most isolates have low carbapenem MICs. Additionally, most phenotypic tests (e.g. CARBA NP, mCIM) have insufficient sensitivity in this species. Furthermore, OXA-23 and OXA-58 are frequent in carbapenemase-producing\n                      <jats:italic toggle=\"yes\">Proteus<\/jats:italic>\n                      but not included in most molecular and immunochromatographic assays for carbapenemase detection in\n                      <jats:italic toggle=\"yes\">Enterobacterales<\/jats:italic>\n                      . The RESIST ACINETO lateral flow test (CORIS BioConcept) was recently developed for the detection of OXA-23, OXA-58\/OXA-40, and NDM in\n                      <jats:italic toggle=\"yes\">Acinetobacter<\/jats:italic>\n                      spp. The aim of this study was to investigate whether a combination of the RESIST-5 O.K.N.V.I., developed for\n                      <jats:italic toggle=\"yes\">Enterobacterales<\/jats:italic>\n                      , and the RESIST ACINETO could improve the detection of carbapenemases in\n                      <jats:italic toggle=\"yes\">P. mirabilis<\/jats:italic>\n                      . Ninety carbapenemase-carrying\n                      <jats:italic toggle=\"yes\">P. mirabilis<\/jats:italic>\n                      isolates were analyzed by whole genome sequencing. All isolates were evaluated by the recently published carbapenemase-detection algorithm for\n                      <jats:italic toggle=\"yes\">P. mirabilis<\/jats:italic>\n                      , followed by RESIST-5 and RESIST ACINETO assay. The algorithm achieved a sensitivity of 97.8%. In comparison, sensitivity\/specificity among targeted carbapenemases was 96.3%\/97.2% for RESIST ACINETO and 96.4%\/100% for RESIST-5. In combination, a sensitivity of 97.8% was achieved. The sensitivity was excellent for OXA-23 (23\/23, 100%) and NDM (18\/18, 100%), but lower for OXA-58 (12\/13, 92.3%) and OXA-48-like (24\/25, 96.0%). The RESIST ACINETO and RESIST-5 performed well in detecting carbapenemases in\n                      <jats:italic toggle=\"yes\">P. mirabilis<\/jats:italic>\n                      , particularly for the emerging OXA-23-producing\n                      <jats:italic toggle=\"yes\">P. mirabilis<\/jats:italic>\n                      . The assays have the potential to improve carbapenemase detection in the routine diagnostic laboratory for this challenging species.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      OXA-23 and OXA-58 are among the most frequent carbapenemases in\n                      <jats:italic toggle=\"yes\">Proteus mirabilis<\/jats:italic>\n                      but are often missed because of the difficult detection. In this article, the performance of immunochromatographic assays for the rapid and easy detection of these enzymes is assessed. The study includes a very large set of molecularly well-characterized isolates. The present work demonstrates that the notoriously challenging detection of carbapenemases in\n                      <jats:italic toggle=\"yes\">P. mirabilis<\/jats:italic>\n                      can be achieved by immunochromatographic assays, enabling laboratories outside of academic centers to reliably detect these emerging carbapenemases in a short time. This will help prevent the further spread of carbapenemase-producing\n                      <jats:italic toggle=\"yes\">P. mirabilis<\/jats:italic>\n                      in hospitals.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01899-25","type":"journal-article","created":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T14:02:31Z","timestamp":1772200951000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Detection of OXA-23 and OXA-58 in\n                    <i>Proteus mirabilis<\/i>\n                    by immunochromatographic assays"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8802-6605","authenticated-orcid":false,"given":"Janina","family":"Noster-Schrader","sequence":"first","affiliation":[{"name":"Carl von Ossietzky University Oldenburg, Institute of Medical Microbiology and Virology","place":["Oldenburg, 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