{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T15:40:34Z","timestamp":1792510834979,"version":"4.5.0"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["GNT1194879"],"award-info":[{"award-number":["GNT1194879"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,10,6]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n The genome of NL6, a multiply antibiotic-resistant\n Acinetobacter baumannii<\/jats:italic>\n ST10:KL49:OCL2 carriage isolate from Vietnam, was sequenced using Nanopore technology, and complete chromosome and plasmid sequences were assembled from the long reads and available short reads. Resistance genes and their locations were identified, and transfer of a conjugative plasmid carrying several resistance genes into a new host was tested. The acquired resistance genes in NL6 were distributed between the chromosome and two of three plasmids present. The chromosome carries multiple copies of several insertion sequences, an incomplete copy of the ISAba1-bounded Tn\n 6250<\/jats:italic>\n that includes the\n sul2<\/jats:italic>\n and\n strAB<\/jats:italic>\n genes, and an integrative element carrying copper resistance genes designated IECu\n R<\/jats:sup>\n . Plasmid pNL6-2 (r3-T5; 15 Kbp) is a Rep_3\/OrfX plasmid that includes a\n tet39 dif<\/jats:italic>\n module, and pNL6-3 (r3-T20; 66.9 Kbp) carries\n aacC2d<\/jats:italic>\n ,\n aphA6<\/jats:italic>\n , and\n bla<\/jats:italic>\n CARB-16<\/jats:sub>\n and a second\n ampC<\/jats:italic>\n gene preceded by an ISAba1. Conjugation of pNL6-3 into derivatives of ATCC17978 was demonstrated, confirming that the\n ampC<\/jats:italic>\n gene confers resistance to third-generation cephalosporins. NL6 was compared to other complete ST10 genomes. Several acquired elements in the chromosome were shared with the ST10 isolate LAC-4 (USA), indicating shared ancestry, but the plasmid content differed. The KL and plasmid content were variable in 17 further complete ST10 genomes downloaded from GenBank. Tn\n 6250<\/jats:italic>\n and IECu\n R<\/jats:sup>\n were only found together in the chromosome of two further KL49 isolates. Antibiotic resistance in ST10\n A. baumannii<\/jats:italic>\n was acquired mainly via plasmid acquisition, but resistance genes varied, and a variety of plasmids was involved.\n <\/jats:p>\n \n IMPORTANCE<\/jats:title>\n \n Members of the CC10 clonal complex of\n Acinetobacter baumannii<\/jats:italic>\n comprising ST10 plus single and double locus variants are known to be particularly virulent. However, antibiotic resistance in members of this group has rarely been examined. Here, determination of the complete genome (chromosome and plasmids) of a representative ST10 isolate from Vietnam allowed the context and location of acquired antibiotic resistance genes and of other mobile genetic elements to be determined. Mobile genetic element locations in completed chromosomes facilitate comparisons of potentially related genomes, revealing those with recent shared ancestry. Differences in plasmid content can also be examined.\n <\/jats:p>\n <\/jats:sec>\n <\/jats:sec>","DOI":"10.1128\/spectrum.01926-26","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T13:00:21Z","timestamp":1788958821000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Complete genome of multiply antibiotic resistant ST10\n Acinetobacter baumannii<\/i>\n isolate NL6 from Vietnam and relationship to available ST10 genomes"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4418-6727","authenticated-orcid":true,"given":"Stephanie J.","family":"Ambrose","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0384j8v12","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Life and Environmental Sciences, The University of Sydney","place":["Sydney, NSW, 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