{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:33:35Z","timestamp":1785407615297,"version":"3.56.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T00:00:00Z","timestamp":1772496000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T00:00:00Z","timestamp":1772496000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/01255-9, 2021\/03684-4"],"award-info":[{"award-number":["2019\/01255-9, 2021\/03684-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2025\/05535-7"],"award-info":[{"award-number":["2025\/05535-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2025\/05645-7"],"award-info":[{"award-number":["2025\/05645-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2022\/12861-0"],"award-info":[{"award-number":["2022\/12861-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/14526-0, 2020\/05146-7"],"award-info":[{"award-number":["2019\/14526-0, 2020\/05146-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2020\/08943-5"],"award-info":[{"award-number":["2020\/08943-5"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88887.990395\/2024-00, 88887.901798\/2023-00"],"award-info":[{"award-number":["88887.990395\/2024-00, 88887.901798\/2023-00"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["175498\/2024-5"],"award-info":[{"award-number":["175498\/2024-5"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,3,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Tacaribe virus (TCRV), a New World arenavirus, is associated with neotropical frugivorous bats, particularly\n                      <jats:italic toggle=\"yes\">Artibeus<\/jats:italic>\n                      spp., and is considered to have zoonotic potential. Here, we report the detection of TCRV in multiple biological compartments of a\n                      <jats:italic toggle=\"yes\">Molossus molossus<\/jats:italic>\n                      (velvety free-tailed bat), an insectivorous species commonly found in Brazilian urban ecosystems. Brain tissues negative for rabies were subjected to high-throughput RNA sequencing, revealing a diverse array of viral taxa, including partial L and S segments of TCRV with respective genome coverages of 68.2% and 65.6%. These sequences shared 90% nucleotide identity and 94% amino acid identity with TCRV reference strains. Phylogenetic reconstruction grouped the newly identified TCRV sequences and the TCRV strain A354 (isolated from a Brazilian\n                      <jats:italic toggle=\"yes\">Artibeus planirostris<\/jats:italic>\n                      bat) within a clade that also includes Tiet\u00ea mammarenavirus strains (Brazilian\n                      <jats:italic toggle=\"yes\">Carollia perspicillata<\/jats:italic>\n                      bat), both of which are genetically divergent members of the Arenaviridae family. The detection of TCRV in\n                      <jats:italic toggle=\"yes\">M. molossus<\/jats:italic>\n                      may indicate previously unrecognized circulation of this virus in insectivorous bats, expanding our understanding of its tissue tropism and host range. This finding is particularly significant given the synanthropic behavior of\n                      <jats:italic toggle=\"yes\">M. molossus<\/jats:italic>\n                      bats and its potential implications for TCRV evolution. Moreover, our results highlight the critical role of untargeted high-throughput sequencing in uncovering overlooked viral diversity, enabling the detection of unexpected pathogens in non-traditional hosts and tissues. While TCRV remains poorly characterized in terms of human pathogenicity, continued surveillance is warranted to assess potential spillover risks.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Understanding which animals carry viruses is key to predicting and preventing disease spread. Here, we report the first detection of Tacaribe virus (TCRV), previously found only in fruit-eating bats, in multiple biological compartments, including the brain, of\n                      <jats:italic toggle=\"yes\">Molossus molossus<\/jats:italic>\n                      , an insect-eating bat common in urban areas of Brazil. This finding expands the known range of both the virus and its possible hosts. By confirming this unexpected host-virus association using original tissue samples, our study provides new insights into how TCRV may circulate in nature. While TCRV is not currently considered a major threat to humans, its detection in a new bat species raises important questions about its transmission, evolution, and potential health impacts. These results emphasize the importance of monitoring diverse bat species, including those not traditionally linked to specific viruses, to better assess emerging virus risks in changing environments.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.01960-25","type":"journal-article","created":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T14:48:18Z","timestamp":1769006898000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Integrated molecular and metatranscriptomic evidence of Tacaribe virus and the brain virome profile of\n                    <i>Molossus molossus<\/i>\n                    bat sampled in Brazil"],"prefix":"10.1128","volume":"14","author":[{"given":"Larissa Le\u00e3o F.","family":"de Sousa","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02k5swt12","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Immunology and Parasitology, Federal University of S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]},{"name":"Rabies Diagnosis Laboratory, Central Laboratory of Public Health - LACEN","place":["Fortaleza-CE, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8579-2091","authenticated-orcid":false,"given":"Rodrigo Lopes","family":"Sanz Duro","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02k5swt12","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Morphology and Genetics, Federal University of S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel","family":"de Oliveira Vaz","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02k5swt12","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Immunology and Parasitology, Federal University of S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]},{"id":[{"id":"https:\/\/ror.org\/036rp1748","id-type":"ROR","asserted-by":"publisher"}],"name":"Interunit Bioinformatics Graduate Program, Institute of Chemistry, University of S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara Lacerda","family":"de Athayde","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02k5swt12","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Morphology and Genetics, Federal University of S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]},{"id":[{"id":"https:\/\/ror.org\/00ay50243","id-type":"ROR","asserted-by":"publisher"}],"name":"Anhembi Morumbi University","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junior Olimpio","family":"Martins","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02k5swt12","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Morphology and Genetics, Federal University of S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna Stefanie Silv\u00e9rio","family":"de Lima Slobosk","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02k5swt12","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Morphology and Genetics, Federal University of S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Dias","family":"Guilardi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02k5swt12","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Immunology and Parasitology, Federal University of S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]},{"id":[{"id":"https:\/\/ror.org\/036rp1748","id-type":"ROR","asserted-by":"publisher"}],"name":"Interunit Bioinformatics Graduate Program, Institute of Chemistry, University of S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Cabral-Miranda","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036rp1748","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Biomedical Sciences, University of S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3771-1565","authenticated-orcid":false,"given":"Edmilson Ferreira","family":"de Oliveira-Filho","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04xrjem68","id-type":"ROR","asserted-by":"publisher"}],"name":"Charit\u00e9-Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin and Humboldt Universit\u00e4t zu Berlin, Institute of Virology","place":["Berlin, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3509-0232","authenticated-orcid":true,"given":"Jan Felix","family":"Drexler","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04xrjem68","id-type":"ROR","asserted-by":"publisher"}],"name":"Charit\u00e9-Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin and Humboldt Universit\u00e4t zu Berlin, Institute of Virology","place":["Berlin, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz M\u00e1rio","family":"Ramos Janini","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02k5swt12","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Immunology and Parasitology, Federal University of S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5734-7601","authenticated-orcid":true,"given":"Ricardo","family":"Dur\u00e3es-Carvalho","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02k5swt12","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Immunology and Parasitology, Federal University of S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]},{"id":[{"id":"https:\/\/ror.org\/02k5swt12","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Morphology and Genetics, Federal University of S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]},{"id":[{"id":"https:\/\/ror.org\/036rp1748","id-type":"ROR","asserted-by":"publisher"}],"name":"Interunit Bioinformatics Graduate Program, Institute of Chemistry, University of S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00201-12"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiac266"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2021.02.046"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1963.12.640"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000133107"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-38202-4"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2023.2192816"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-87108-2"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00520-24"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2812.220980"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/s42522-024-00124-6"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.70173"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2024.107309"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1001191"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1315419110"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-virology-092818-015613"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061950"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-11384-w"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty695"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00605-24"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2003.08.016"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-031-15640-3_8"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001137"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2020.09.025"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01960-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.01960-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T14:04:23Z","timestamp":1772546663000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.01960-25"}},"subtitle":[],"editor":[{"given":"J. J.","family":"Miranda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,3,3]]},"references-count":24,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3,3]]}},"alternative-id":["10.1128\/spectrum.01960-25"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.01960-25","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,3]]},"assertion":[{"value":"2025-06-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01960-25"}}