{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T19:27:13Z","timestamp":1780082833016,"version":"3.54.0"},"reference-count":15,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T00:00:00Z","timestamp":1738627200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T00:00:00Z","timestamp":1738627200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"crossref","award":["NRF-2022R1A2B502001716"],"award-info":[{"award-number":["NRF-2022R1A2B502001716"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,2,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              There is a critical need for treatment strategies to combat carbapenem-resistant Gram-negative pathogens. This study investigates the efficacy of combining low concentrations of colistin with tigecycline against colistin- and carbapenem-resistant\n              <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n              and\n              <jats:italic>Acinetobacter baumannii<\/jats:italic>\n              strains. We used two strains of KPC-2-producing\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              and OXA-23-producing\n              <jats:italic>A. baumannii<\/jats:italic>\n              , both of which are highly colistin-resistant. In the\n              <jats:italic>in vitro<\/jats:italic>\n              time-killing assays, monotherapy with colistin (2 mg\/L) and tigecycline (4 or 8 mg\/L) was ineffective against all strains. However, the combination of colistin (2 mg\/L) and tigecycline (4 or 8 mg\/L) inhibited bacterial growth. Survival analysis of\n              <jats:italic>Galleria mellonella<\/jats:italic>\n              larvae also demonstrated the efficacy of low concentrations of colistin and tigecycline against colistin- and carbapenem-resistant strains. All larvae survived when treated with a combination of colistin and tigecycline, whereas monotherapy with either colistin or tigecycline did not increase larval survival rates. This study proposes using a combination of tigecycline and colistin at clinical concentrations to treat carbapenem-resistant\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              and\n              <jats:italic>A. baumannii,<\/jats:italic>\n              regardless of colistin resistance.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>Colistin resistance in carbapenem-resistant Gram-negative pathogens is a serious challenge in clinical settings. Our study showed that low concentration of colistin can be effective when combined with tigecycline. It presents the possibility of an available treatment option for antibiotic-resistant pathogens.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.02021-24","type":"journal-article","created":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T14:18:56Z","timestamp":1734617936000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Effect of colistin-tigecycline combination on colistin-resistant and carbapenem-resistant\n            <i>Klebsiella pneumoniae<\/i>\n            and\n            <i>Acinetobacter baumannii<\/i>"],"prefix":"10.1128","volume":"13","author":[{"given":"Suyeon","family":"Park","sequence":"first","affiliation":[{"name":"Department of Microbiology, Sungkyunkwan University School of Medicine","place":["Suwon, South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhong","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sungkyunkwan University School of Medicine","place":["Suwon, South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0978-1937","authenticated-orcid":true,"given":"Kwan Soo","family":"Ko","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Sungkyunkwan University School of Medicine","place":["Suwon, South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1159798"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00078-15"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00055"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.823684"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1177\/2049936115621709"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11081912"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2023.106713"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2023.107017"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2019.00151"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2010.10.031"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0270908"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkz034"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkz552"},{"key":"e_1_3_2_15_2","volume-title":"Supplement M100-S33Performance standards for antimicrobial susceptibility testing: thirty-third informational","author":"Clinical and Laboratory Standards Institute","year":"2023","unstructured":"Clinical and Laboratory Standards Institute. 2023. Supplement M100-S33. Performance standards for antimicrobial susceptibility testing: thirty-third informational. CLSI, Wayne, PA."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-023-00910-w"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.02021-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.02021-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T14:07:06Z","timestamp":1738678026000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.02021-24"}},"subtitle":[],"editor":[{"given":"Ruth M.","family":"Hall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,2,4]]},"references-count":15,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,2,4]]}},"alternative-id":["10.1128\/spectrum.02021-24"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.02021-24","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,4]]},"assertion":[{"value":"2024-08-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02021-24"}}