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In this study, we identified a\n                    <jats:underline>v<\/jats:underline>\n                    irulence-related\n                    <jats:underline>D<\/jats:underline>\n                    eoR-family\n                    <jats:underline>t<\/jats:underline>\n                    ranscriptional\n                    <jats:underline>r<\/jats:underline>\n                    egulator (VdtR) and an erythronate metabolic pathway responsible for four-carbon acid sugar metabolism of D-erythronate and L-threonate in\n                    <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                    . We found that VdtR plays an important role in\n                    <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                    intracellular survival and trafficking to the endoplasmic reticulum in RAW 264.7 macrophages and in virulence in a mouse model. More importantly, we found that VdtR negatively regulates the erythronate metabolic pathway to promote extracellular proliferation of\n                    <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                    , depending on utilization of D-erythronate, an oxidative product of erythritol in the host. In a pregnant mouse model, the erythronate metabolic pathway was shown to cooperate with erythritol metabolism and play a crucial role in\n                    <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                    proliferation in the placenta, inducing placentitis and finally resulting in abortion or stillbirth. Our results demonstrate that, in addition to erythritol, erythronate is a preferred carbon source for\n                    <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                    utilization to promote its extracellular proliferation. This discovery updates the information on the preferential colonization of reproductive organs by\n                    <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                    and provides a novel insight into the\n                    <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                    -associated induction of abortion in pregnant animals.\n                  <\/jats:p>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                      is an intracellular parasitic bacterium causing zoonosis, which is distributed worldwide and mainly characterized by reproductive disorders. Erythritol is found in allantoic fluid, chorion, and placenta of aborted animals, preferentially utilized by\n                      <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                      to cause infertility and abortion. However, the erythritol metabolism-defected mutant was unable to function as a vaccine strain due to its residual virulence. Here, we found that erythronate, an oxidative product of erythritol in the host, was also preferentially utilized by\n                      <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                      relying on the function of a deoxyribonucleoside regulator-family transcriptional regulator VdtR. Erythronate utilization activates VdtR regulation of the erythronate metabolic pathway to promote\n                      <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                      extracellular proliferation, inducing placentitis\/abortion in mice. Double mutations on\n                      <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                      erythritol and D-erythronate metabolisms significantly reduced bacterial virulence. This study revealed a novel mechanism of\n                      <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                      infection-induced abortion, thus providing a new clue for the study of safer\n                      <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                      attenuated vaccines.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02074-23","type":"journal-article","created":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T09:01:22Z","timestamp":1693990882000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Erythronate utilization activates VdtR regulating its metabolism to promote\n                    <i>Brucella<\/i>\n                    proliferation, inducing abortion in mice"],"prefix":"10.1128","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0694-6277","authenticated-orcid":false,"given":"Yi","family":"Yin","sequence":"first","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS)","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Fang","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS)","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengmin","family":"Lian","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS)","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Zuo","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS)","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai","family":"Hu","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS)","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangdong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS)","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chan","family":"Ding","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS)","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxing","family":"Tian","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS)","place":["Shanghai, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1586-4277","authenticated-orcid":false,"given":"Shengqing","family":"Yu","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS)","place":["Shanghai, China"]},{"name":"Veterinary Bio-Pharmaceutical, Jiangsu Agri-Animal Husbandry Vocational College, Jiangsu Key Laboratory for High-Tech Research and Development of Veterinary Biopharmaceuticals","place":["Taizhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-30745-1_17:315-456"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11030279"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2007.11.023"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01663-14"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2011.08.011"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2004-1513"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/89.3.266"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/193047a0"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1414622111"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.121.2.619-630.1975"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/115.3.312"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01088"},{"key":"e_1_3_5_14_2","first-page":"91","article-title":"A preliminary report on the efficacy of adult cattle vaccination using strain 19 in selected dairy herds in florida","author":"Nicoletti P","year":"1976","unstructured":"Nicoletti P . 1976. 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