{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T07:45:54Z","timestamp":1769586354523,"version":"3.49.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T00:00:00Z","timestamp":1738627200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T00:00:00Z","timestamp":1738627200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,2,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Carbapenem-resistant Enterobacterales (CRE) are divided into two distinct groups: carbapenemase-producing (CPE) and non-carbapenemase-producing (non-CPE). The population of non-CPE growing on CPE selective plates during routine screening is usually not reported and is not well defined. This study aimed to characterize non-CPE isolates growing on those plates. Isolates were collected from two medical institutions in Israel between May and November 2022. Species identification and antibiotic susceptibility testing were performed using VITEK systems. Further analyses focused on\n              <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n              and\n              <jats:italic>Escherichia coli<\/jats:italic>\n              which were the most common isolates. Meropenem MIC was determined by E-test. Fourier-transform infrared spectroscopy (FTIR) was used to analyze phenotypic similarity, and whole genome sequencing was conducted on a subset of non-CPE isolates. Of 260 isolates growing on selective CPE screening plates, 60.4% were non-CPE. The most common non-CP isolates were\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              (65.6%) followed by\n              <jats:italic>E. coli<\/jats:italic>\n              (20.4%). Of the non-CP\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              and\n              <jats:italic>E. coli<\/jats:italic>\n              isolates, 78.2% were susceptible to meropenem. All non-CP\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              and\n              <jats:italic>E. coli<\/jats:italic>\n              isolates were multidrug-resistant (MDR) and belonged to multiple FTIR clusters. The 21 sequenced non-CPE isolates carried multiple mechanisms of resistance. Isolates had multiple beta-lactamases and all had penicillin-binding protein modifications and porin mutations; in meropenem-resistant\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              isolates, both Ompk35 and Ompk36 were mutated. The majority of isolates growing on selective CPE screening plates are non-CPE but are MDR. Laboratory reporting of these MDR bacteria might be useful for guiding treatment and prophylaxis when indicated, as well as for infection control.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>Selective screening plates for carbapenemase-producing Enterobacterales (CPE) are used to detect CPE carriers for infection control purposes. We characterized non-CPE isolates that grew on selective CPE screening plates, which are intended to filter them out. We found that 60% of isolates that grew on these plates were not CPE. They included both meropenem-susceptible and meropenem-resistant isolates and were multidrug-resistant with multiple resistance mechanisms. These test results, which are usually not reported by laboratories, may be clinically valuable.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.02079-24","type":"journal-article","created":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T14:01:27Z","timestamp":1736863287000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Characterization of Enterobacterales growing on selective CPE screening plates with a focus on non-carbapenemase-producing strains"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-8180-9134","authenticated-orcid":false,"given":"Reut","family":"Efrati Epchtien","sequence":"first","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control, Israel Ministry of Health","place":["Tel Aviv, Israel"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Temkin","sequence":"additional","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control, Israel Ministry of Health","place":["Tel Aviv, Israel"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2488-3720","authenticated-orcid":false,"given":"Mor N.","family":"Lurie-Weinberger","sequence":"additional","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control, Israel Ministry of Health","place":["Tel Aviv, Israel"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ophir","family":"Kastel","sequence":"additional","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control, Israel Ministry of Health","place":["Tel Aviv, Israel"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alona","family":"Keren-Paz","sequence":"additional","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control, Israel Ministry of Health","place":["Tel Aviv, Israel"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Schwartz","sequence":"additional","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control, Israel Ministry of Health","place":["Tel Aviv, Israel"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yehuda","family":"Carmeli","sequence":"additional","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control, Israel Ministry of Health","place":["Tel Aviv, Israel"]},{"name":"Faculty of Medicine, Tel Aviv University","place":["Tel Aviv-Yafo, Israel"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0151897"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S260804"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2020.08.002"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofy150"},{"key":"e_1_3_4_6_2","doi-asserted-by":"crossref","unstructured":"Centers for Disease Control. 2019. Antibiotic resistance threats in the United States 2019. Available from: https:\/\/doi.org\/10.15620\/cdc:82532","DOI":"10.15620\/cdc:82532"},{"key":"e_1_3_4_7_2","unstructured":"WHO bacterial priority pathogens list 2024: bacterial pathogens of public health importance to guide research development and strategies to prevent and control antimicrobial resistance. 2024. 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