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Limited effective therapeutic options make it difficult to treat this emerging pathogen. In this context, combinations of different antifungal drugs are considered a promising therapeutic alternative. The aim of this work was to analyze the antifungal activity of combinations of amphotericin B and echinocandins against five clinical blood isolates of\n            <jats:italic>C. auris<\/jats:italic>\n            . One of these isolates showed an aggregative phenotype already described in\n            <jats:italic>C. auris<\/jats:italic>\n            species, forming large aggregates of cells that are very difficult to disintegrate. First, the\n            <jats:italic>in vitro<\/jats:italic>\n            activity of these drugs was evaluated both in monotherapy and in combination, and results showed synergistic interactions between amphotericin B and echinocandins in most cases, although higher minimum inhibitory concentration (MIC) values were observed against the aggregative isolate compared to non-aggregative counterparts. The most promising drug combinations were then selected for\n            <jats:italic>in vivo<\/jats:italic>\n            activity evaluation using a\n            <jats:italic>Caenorhabditis elegans<\/jats:italic>\n            model of candidiasis and by determination of survival rates. The combination of amphotericin B and caspofungin showed the strongest protective effect during\n            <jats:italic>C. elegans<\/jats:italic>\n            infection with\n            <jats:italic>C. auris<\/jats:italic>\n            blood isolates, achieving up to 99%\n            <jats:italic>C<\/jats:italic>\n            .\n            <jats:italic>elegans<\/jats:italic>\n            survival. Although the MIC values for micafungin were low\n            <jats:italic>in vitro<\/jats:italic>\n            , these drug concentrations had no effect\n            <jats:italic>in vivo<\/jats:italic>\n            , and the combination of amphotericin B and micafungin was the least effective. The results of this study showed that the combination amphotericin B and echinocandins might be a promising therapeutic approach for the treatment of invasive candidiasis caused by\n            <jats:italic>C. auris<\/jats:italic>\n            . Moreover, the nematode\n            <jats:italic>C. elegans<\/jats:italic>\n            is a suitable alternative model for screening of new therapeutic agents capable of overcoming multidrug-resistant\n            <jats:italic>C. auris<\/jats:italic>\n            infections.\n          <\/jats:p>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Multidrug resistance is a rising problem among non-\n              <jats:italic>Candida albicans<\/jats:italic>\n              species, such as\n              <jats:italic>Candida auris<\/jats:italic>\n              . This therapeutic problem has been very important during the COVID-19 pandemic. The World Health Organization has included\n              <jats:italic>C. auris<\/jats:italic>\n              in its global priority list of health-threatening fungi, to study this emerging multidrug-resistant species and to develop effective alternative therapies. In the present study, the synergistic effect of the combination of amphotericin B and echinocandins has been demonstrated against blood isolates of\n              <jats:italic>C. auris<\/jats:italic>\n              . Different susceptibility responses were also observed between aggregative and non-aggregative phenotypes. The antifungal activity of these drug combinations against\n              <jats:italic>C. auris<\/jats:italic>\n              was also demonstrated in the\n              <jats:italic>Caenorhabditis elegans<\/jats:italic>\n              host model of candidiasis, confirming the suitability and usefulness of this model in the search for solutions to antimicrobial resistance.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.02086-23","type":"journal-article","created":{"date-parts":[[2023,11,29]],"date-time":"2023-11-29T14:01:11Z","timestamp":1701266471000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Efficacy of the combination of amphotericin B and echinocandins against\n            <i>Candida auris in vitro<\/i>\n            and in the\n            <i>Caenorhabditis elegans<\/i>\n            host 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