{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:30:08Z","timestamp":1785407408532,"version":"3.56.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Science and Technology Plan Project In Guangzhou","award":["2025A04J7118"],"award-info":[{"award-number":["2025A04J7118"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32200025"],"award-info":[{"award-number":["32200025"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fujian Province Natural Science Foundation of China","award":["No. 2021J01529"],"award-info":[{"award-number":["No. 2021J01529"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,10,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The quorum-sensing (QS) system in\n                      <jats:italic toggle=\"yes\">Methylobacterium oryzae<\/jats:italic>\n                      CBMB20, an endophytic bacterium associated with rice (\n                      <jats:italic toggle=\"yes\">Oryza sativa<\/jats:italic>\n                      L.), plays a critical role in regulating bacterial behaviors essential for plant growth promotion and adaptation. This study aimed to elucidate the functional role of the MorI\/MorR QS system in\n                      <jats:italic toggle=\"yes\">M. oryzae<\/jats:italic>\n                      CBMB20 and its potential application as a bioinoculant. We identified and characterized two QS signals, 3-OH-C12-HSL and 3-oxo-C12-HSL, synthesized by the MorI enzyme. The MorR receptor was found to preferentially respond to 3-OH-C12-HSL, indicating a high degree of specificity in QS signaling. The deletion mutants of\n                      <jats:italic toggle=\"yes\">morI<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">morR<\/jats:italic>\n                      exhibited significant changes in exopolysaccharides (EPS), motility, and methanol utilization, suggesting that the MorI\/MorR system is crucial for bacterial survival and adaptation. Transcriptome analysis revealed that MorR acts as a repressor, controlling the expression of numerous genes, many of which are upregulated upon its deletion. Our findings highlight the multifaceted role of the MorI\/MorR QS system in\n                      <jats:italic toggle=\"yes\">M. oryzae<\/jats:italic>\n                      CBMB20, influencing key biological functions such as EPS production, motility, and methanol utilization. The modulation of these traits through QS could enhance the bacterium\u2019s effectiveness as a bioinoculant for promoting plant growth. This study contributes to the understanding of how QS systems can be harnessed to improve the efficacy of plant growth-promoting bacteria in agricultural settings, offering insights into the potential for genetic manipulation to optimize bioinoculant performance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      This study provides critical insights into microbial communication by functionally characterizing the MorI\/MorR quorum-sensing (QS) system in\n                      <jats:italic toggle=\"yes\">Methylobacterium oryzae<\/jats:italic>\n                      CBMB20, a plant-beneficial methylotroph. We identify 3-OH-C12-AHL as a key long-chain signal governing exopolysaccharides biosynthesis, swimming motility, and methanol metabolism traits pivotal for host colonization. These findings not only elucidate novel regulatory mechanisms in plant-associated bacteria but also pave the way for engineering QS-driven strategies, such as synthetic consortia or targeted microbiome interventions, to enhance sustainable agricultural practices.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02117-25","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T13:00:56Z","timestamp":1757682056000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["The role of MorI\/MorR quorum sensing in\n                    <i>Methylobacterium oryzae<\/i>\n                    CBMB20: modulating bacterial functions for enhanced adaptability"],"prefix":"10.1128","volume":"13","author":[{"given":"Qiying","family":"Deng","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05v9jqt67","id-type":"ROR","asserted-by":"publisher"}],"name":"Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Center, South China Agricultural University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zheng","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences","place":["Xiamen, China"]},{"id":[{"id":"https:\/\/ror.org\/00mcjh785","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Marine Environmental Science and College of the Environment and Ecology, Xiamen University","place":["Xiamen, 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