{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:51:45Z","timestamp":1786117905247,"version":"build-2736575974"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC2307301"],"award-info":[{"award-number":["2023YFC2307301"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The treatment of drug-resistant tuberculosis (DR-TB) faces significant challenges. Novel antituberculosis drugs such as bedaquiline (BDQ), pretomanid (Pa824), and delamanid (DLM) offer a promising treatment for DR-TB. However, the synergistic effects of these drugs have been insufficiently explored. In this research, we evaluated the synergistic effects of two dual-drug combinations (BDQ-Pa824 and BDQ-DLM) and four triple-drug combinations (BDQ-DLM-clofazimine [CFZ], BDQ-DLM-linezolid [LZD], BDQ-Pa824-CFZ, and BDQ-Pa824-LZD) against 30\n                      <jats:italic toggle=\"yes\">Mycobacterium tuberculosis<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">MTB<\/jats:italic>\n                      ) strains isolated from TB patients. The results indicated that the synergistic antimycobacterial effects of BDQ-DLM-LZD and BDQ-Pa824-LZD show no statistically significant difference, suggesting that BDQ-DLM-LZD could be considered a potential therapeutic alternative for DR-TB. This provides evidence for the development of more flexible treatment options for DR-TB.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        The rapid emergence of antimicrobial resistance in\n                        <jats:italic toggle=\"yes\">Mycobacterium tuberculosis<\/jats:italic>\n                        (MTB) presents a critical challenge for global public health. Given that the synergistic effects of antituberculosis drugs against drug-resistant tuberculosis have not been systematically analyzed. In this research, we evaluated the synergistic antimycobacterial efficacy of the new optimized antituberculosis regimen (BDQ-DLM-LZD or BDQ-DLM-CFZ) and the standard regimen (BDQ-Pa824-LZD or BDQ-Pa824-CFZ) for TB strains. This highlights the therapeutic substitutability of BDQ-DLM or BDQ-Pa824-based triple-drug regimens for TB strains.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02128-25","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T13:04:31Z","timestamp":1783688671000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Synergism in drug combination of new tuberculosis antibiotics for drug-resistant\n                    <i>Mycobacterium tuberculosis in vitro<\/i>"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3360-2297","authenticated-orcid":false,"given":"Run","family":"Huang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03rc6as71","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Tuberculosis, Shanghai Pulmonary Hospital, School of Medicine, Tongji University","place":["Shanghai, People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5756-0166","authenticated-orcid":true,"given":"Yifan","family":"He","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03rc6as71","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Tuberculosis, Shanghai Pulmonary Hospital, School of Medicine, Tongji University","place":["Shanghai, People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinghui","family":"Yang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03rc6as71","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, Shanghai Pulmonary Hospital, School of Medicine, Tongji University","place":["Shanghai, People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yidian","family":"Liu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03rc6as71","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Tuberculosis, Shanghai Pulmonary Hospital, School of Medicine, Tongji University","place":["Shanghai, People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3445-920X","authenticated-orcid":true,"given":"Qin","family":"Sun","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03rc6as71","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Tuberculosis, Shanghai Pulmonary Hospital, School of Medicine, Tongji University","place":["Shanghai, People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0825-973X","authenticated-orcid":false,"given":"Ruoyan","family":"Ying","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03rc6as71","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Tuberculosis, Shanghai Pulmonary Hospital, School of Medicine, Tongji University","place":["Shanghai, People\u2019s Republic of China"]},{"id":[{"id":"https:\/\/ror.org\/03rc6as71","id-type":"ROR","asserted-by":"publisher"}],"name":"Shanghai Key Laboratory of Tuberculosis, Shanghai Pulmonary Hospital, School of Medicine, Tongji University","place":["Shanghai, People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2099-8205","authenticated-orcid":false,"given":"Wei","family":"Sha","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03rc6as71","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Tuberculosis, Shanghai Pulmonary Hospital, School of Medicine, Tongji University","place":["Shanghai, People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-022-00731-y"},{"key":"e_1_3_3_3_2","unstructured":"World Health Organization. 2025. 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