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Pulsed-field gel electrophoresis (PFGE) is still used in clinical laboratories despite the increased accessibility of whole-genome sequencing (WGS). As PFGE equipment is no longer commercially available, clinical laboratories need alternative tools. Highly standardized multilocus sequence typing (MLST) is one option. However, the original MLST scheme for\n              <jats:italic>E. faecium<\/jats:italic>\n              , designed in 2002, showed inconsistencies with WGS-based typing. Therefore, the new Bezd\u00ed\u010dek MLST scheme, which offers more accurate genetic similarity based on genome-wide data, has recently been proposed. To clarify its clinical utility in analyzing nosocomial VREfm transmission, we compared both MLST schemes with PFGE using 68 VREfm isolates collected during an outbreak at a Japanese tertiary medical center in 2019. PFGE analysis identified nine clusters among the 68 strains, including two predominant clusters. The original scheme identified five sequence types (ST\n              <jats:sub>O<\/jats:sub>\n              s), of which 82.4% (56\/68) were ST\n              <jats:sub>O<\/jats:sub>\n              192. The Bezd\u00ed\u010dek scheme identified nine sequence types (ST\n              <jats:sub>B<\/jats:sub>\n              s), subdividing the original ST\n              <jats:sub>O<\/jats:sub>\n              192 into ST\n              <jats:sub>B<\/jats:sub>\n              1162 (30\/56), ST\n              <jats:sub>B<\/jats:sub>\n              610 (25\/56), and ST\n              <jats:sub>B<\/jats:sub>\n              895 (1\/56). Simpson\u2019s index of diversity values were 0.635, 0.317, and 0.648 for PFGE, the original scheme, and the Bezd\u00ed\u010dek scheme, respectively. Combining the Bezd\u00ed\u010dek scheme with admission records provided clearer outbreak visualization, indicating that two distinct ST\n              <jats:sub>B<\/jats:sub>\n              s independently caused sequential outbreaks. With high discriminatory power comparable with PFGE and global availability, the Bezd\u00ed\u010dek scheme is a practical and valuable tool for controlling nosocomial VREfm infections in clinical laboratories.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                In areas where vancomycin-resistant\n                <jats:italic>Enterococcus faecium<\/jats:italic>\n                is common, hospital-acquired infections pose a considerable threat to patients\u2019 lives owing to treatment difficulties. Although whole-genome sequencing-based typing has logically become the new reference standard and its accessibility is growing, many clinical laboratories still lack the fundamental resources to exploit its full potential. Limited availability of the traditional pulsed-field gel electrophoresis test in clinical settings has necessitated the use of alternative tools such as Bezd\u00ed\u010dek multilocus sequence typing. This study tested the clinical utility of the Bezd\u00ed\u010dek scheme by comparing it with pulsed-field gel electrophoresis. Designed using Czech isolates, this scheme showed comparable discriminatory powers with the traditional method for geographically distinct Japanese isolates and clearly visualized outbreaks. These findings suggest that the Bezd\u00ed\u010dek scheme is a potential alternative to pulsed-field gel electrophoresis for identifying hospital transmission of vancomycin-resistant\n                <jats:italic>Enterococcus faecium<\/jats:italic>\n                in clinical laboratories.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.02131-24","type":"journal-article","created":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T14:05:24Z","timestamp":1733839524000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["New multilocus sequence typing scheme for\n            <i>Enterococcus faecium<\/i>\n            reveals sequential outbreaks of vancomycin-resistant\n            <i>E. faecium<\/i>\n            ST1162 and ST610 in a Japanese tertiary medical 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