{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T16:39:04Z","timestamp":1780504744228,"version":"3.54.1"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T00:00:00Z","timestamp":1736208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T00:00:00Z","timestamp":1736208000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,1,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n The colonization of\n Enterococcus<\/jats:italic>\n in the female vagina leads to neonatal and pediatric enterococcal septicemia. Linezolid (LZD) is a kind of mainstream drug for treating multidrug-resistant Gram-positive infections.\n OptrA<\/jats:italic>\n is the main LZD-resistance gene at\n Enterococci<\/jats:italic>\n in human isolates. It is essential to explore the prevalence of\n optrA<\/jats:italic>\n -carrying\n Enterococcus<\/jats:italic>\n in vaginal secretions of late pregnant women and the drug resistance of\n optrA<\/jats:italic>\n . From May to June 2023, this study recruited 340 volunteers in late pregnancy (35\u201340 weeks of pregnancy) to provide non-repetitive vaginal discharge samples. Luria-Bertani broth and florfenicol (10 \u00b5g\/mL) were used to enrich bacteria.\n Enterococci<\/jats:italic>\n was identified through time-of-flight mass spectrometry. Additionally, antimicrobial susceptibility, polymerase chain reaction, and next-generation sequencing assays were applied to this study. Fifty-four\n optrA<\/jats:italic>\n -carrying\n Enterococcus<\/jats:italic>\n strains were obtained, the proportion of the whole vagina of late pregnant women was 15.88% (54 out of 340), and\n Enterococcus faecalis<\/jats:italic>\n account the highest proportion. All\n optrA<\/jats:italic>\n -carrying\n Enterococcus<\/jats:italic>\n were resistant to at least three drugs. Tetracycline, chloramphenicol, erythromycin, and LZD have higher bacterial resistance rates. Genetic environment analysis revealed that IS\n 1216E<\/jats:italic>\n ,\n fexA<\/jats:italic>\n , and\n erm<\/jats:italic>\n (A) may synergistically exert multidrug resistance with\n optrA<\/jats:italic>\n . It is necessary to strengthen the surveillance of\n optrA<\/jats:italic>\n -carrying\n Enterococcus<\/jats:italic>\n in pregnant women. This study provides scientific support for controlling hospital infections and managing antibiotic-resistant bacteria, and provides a scientific basis for rational clinical medication.\n <\/jats:p>\n \n IMPORTANCE<\/jats:title>\n \n The disruption of cervicovaginal microbiota homeostasis is considered a key factor in causing imbalance in the microenvironment, leading to inflammation, transmission of infections, and illness.\n Enterococcus<\/jats:italic>\n is considered a major cause of healthcare-related infections globally. It has resistance to multiple antimicrobial drugs, which pose significant challenges for clinical treatment. Therefore, it is crucial to assess the prevalence of\n optrA<\/jats:italic>\n -carrying\n Enterococcus<\/jats:italic>\n in vaginal secretions of late pregnant women and the drug resistance of\n optrA<\/jats:italic>\n . This study detected 15.88% of\n optrA<\/jats:italic>\n -carried\n Enterococci<\/jats:italic>\n in 340 pregnant women. Furthermore, we found that\n optrA<\/jats:italic>\n -carrying\n Enterococcus<\/jats:italic>\n strains are highly resistant to tetracycline, chloramphenicol, erythromycin, and Linezolid. Additionally, genetic environment analysis revealed that\n IS1216E<\/jats:italic>\n ,\n fexA<\/jats:italic>\n , and\n erm<\/jats:italic>\n (A) may synergistically exert multidrug resistance with\n optrA<\/jats:italic>\n . This study provides scientific support for controlling hospital infections and managing antibiotic-resistant bacteria and provides a scientific basis for rational clinical medication.\n <\/jats:p>\n <\/jats:sec>\n <\/jats:sec>","DOI":"10.1128\/spectrum.02135-24","type":"journal-article","created":{"date-parts":[[2024,11,29]],"date-time":"2024-11-29T15:47:07Z","timestamp":1732895227000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["The prevalence of\n optrA<\/i>\n -carrying\n Enterococci<\/i>\n in the vaginal micro-ecology of pregnant women in late pregnancy"],"prefix":"10.1128","volume":"13","author":[{"given":"Yanjun","family":"Xie","sequence":"first","affiliation":[{"name":"Affiliated Xiaoshan Hospital, Hangzhou Normal University","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangyi","family":"Xu","sequence":"additional","affiliation":[{"name":"Affiliated Xiaoshan Hospital, Hangzhou Normal University","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huali","family":"Dong","sequence":"additional","affiliation":[{"name":"Affiliated Xiaoshan Hospital, Hangzhou Normal University","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianfeng","family":"Mao","sequence":"additional","affiliation":[{"name":"Affiliated Xiaoshan Hospital, Hangzhou Normal University","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7834-3053","authenticated-orcid":true,"given":"Chuanling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Affiliated Xiaoshan Hospital, Hangzhou Normal University","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1124591"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.24272\/j.issn.2095-8137.2019.068"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12155069"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/imt2.185"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1163312"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00058-18"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.gpp3-0053-2018"},{"key":"e_1_3_3_9_2","unstructured":"Jun L. 2023. 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