{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T14:35:50Z","timestamp":1785249350741,"version":"3.55.0"},"reference-count":59,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T00:00:00Z","timestamp":1645574400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T00:00:00Z","timestamp":1645574400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100007465","name":"Universitetet i Troms\u00f8","doi-asserted-by":"publisher","award":["Personal Overseas Research Grant"],"award-info":[{"award-number":["Personal Overseas Research Grant"]}],"id":[{"id":"10.13039\/100007465","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"UKRI | Medical Research Council","doi-asserted-by":"publisher","award":["MR\/N013514\/1"],"award-info":[{"award-number":["MR\/N013514\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"UKRI | Medical Research Council","doi-asserted-by":"publisher","award":["MR\/S004793\/1"],"award-info":[{"award-number":["MR\/S004793\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000272","name":"National Institute for Health Research","doi-asserted-by":"publisher","award":["NIHR200632"],"award-info":[{"award-number":["NIHR200632"]}],"id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2022,2,23]]},"abstract":"<jats:p>\n            By analyzing transposition activity within our MGE-free recipient, we can gain insights into the interaction and evolution of multidrug resistance-conferring MGEs following conjugation, including the movement of multiple ISs, the formation of composite transposons, and cointegration and\/or recombination between different replicons in the same cell. This combination of recipient and entrapment vector will allow fine-scale experimental studies of factors affecting intracellular transposition and MGE formation in and from ARG-encoding MGEs from multiple species of clinically relevant\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/spectrum.02140-21","type":"journal-article","created":{"date-parts":[[2022,1,19]],"date-time":"2022-01-19T15:37:12Z","timestamp":1642606632000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Intracellular Transposition and Capture of Mobile Genetic Elements following Intercellular Conjugation of Multidrug Resistance Conjugative Plasmids from Clinical\n            <i>Enterobacteriaceae<\/i>\n            Isolates"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4445-8529","authenticated-orcid":true,"given":"Supathep","family":"Tansirichaiya","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand"},{"name":"Department of Clinical Dentistry, Faculty of Health Sciences, UiT the Arctic University of Norway, Troms\u00f8, Norway"},{"name":"Centre for New Antimicrobial Strategies, UiT the Arctic University of Norway, Troms\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard N.","family":"Goodman","sequence":"additional","affiliation":[{"name":"Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Issra","family":"Bulgasim","sequence":"additional","affiliation":[{"name":"Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5525-2614","authenticated-orcid":true,"given":"\u00d8rjan","family":"Samuelsen","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, UiT the Arctic University of Norway, Troms\u00f8, Norway"},{"name":"Norwegian National Advisory Unit on Detection of Antimicrobial Resistance, Department of Microbiology and Infection Control, University Hospital of North Norway, Troms\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Al-Haroni","sequence":"additional","affiliation":[{"name":"Department of Clinical Dentistry, Faculty of Health Sciences, UiT the Arctic University of Norway, Troms\u00f8, Norway"},{"name":"Centre for New Antimicrobial Strategies, UiT the Arctic University of Norway, Troms\u00f8, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0760-3088","authenticated-orcid":true,"given":"Adam P.","family":"Roberts","sequence":"additional","affiliation":[{"name":"Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/trstmh\/traa173"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks192"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2020.08.018"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03205-z"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02381-17"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm215"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.4.937"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep15082"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24041-8"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa390"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-83081-8"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.164.2.918-921.1985"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(96)00407-6"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28603-0"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/jam.14837"},{"key":"e_1_3_3_17_2","unstructured":"Tansirichaiya S. 2017. Investigation of mobile genetic elements and antimicrobial resistance genes in human oral metagenomic DNA. PhD thesis University College London London UK."},{"key":"e_1_3_3_18_2","first-page":"139","article-title":"Entrapment vectors\u2014how to capture a functional transposable element","volume":"53","author":"So\u0142yga A","year":"2004","unstructured":"So\u0142yga A, Bartosik D. 2004. Entrapment vectors\u2014how to capture a functional transposable element. Pol J Microbiol 53:139\u2013144.","journal-title":"Pol J Microbiol"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.6.2908"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)63789-0"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0187832"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2008.08.001"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02636-14"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2020.102530"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1006\/plas.2000.1483"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1995.tb07381.x"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.13.3753-3763.2003"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1006\/plas.1997.1289"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0032277"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1989.tb00247.x"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-79795-8_1"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2017-0171"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6976.12067"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2021.1906163"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2020.105894"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01344-16"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00918-16"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000158"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2018.0415"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02548-12"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13774"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/108.2.319"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb00225.x"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13100-019-0182-3"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00088-21"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.20.6070-6073.1996"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkx540"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa345"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000224"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02412-14"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-9-38"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0076-6879(01)36578-3"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj014"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-017-1469-3"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1002\/cpbi.102"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0554-6_12"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-402"},{"key":"e_1_3_3_59_2","unstructured":"Li H. 2013. Aligning sequence reads clone sequences and assembly contigs with BWA-MEM. arXiv 1303.3997v2 [q-bio.GN]. https:\/\/github.com\/lh3\/bwa."},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.02140-21","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.02140-21","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T09:01:41Z","timestamp":1646730101000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.02140-21"}},"subtitle":[],"editor":[{"given":"Pablo","family":"Power","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,2,23]]},"references-count":59,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,2,23]]}},"alternative-id":["10.1128\/spectrum.02140-21"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.02140-21","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,23]]},"assertion":[{"value":"2021-11-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02140-21"}}