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Studying IAVs requires methods to detect the presence of the virus in infected cells or animal models. Recombinant IAV-expressing fluorescent proteins has allowed monitoring of viral infection in cultured cells and\n                      <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                      in the organs of infected animals. However, fluorescent-expressing IAVs are often attenuated and are not suited for the imaging of infected animals using\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      imaging systems (IVISs). To overcome this limitation, we generated a recombinant pandemic influenza A\/California\/04\/2009 H1N1 (pH1N1)-expressing nanoluciferase (Nluc) from the non-structural viral segment, hereafter referred to as pH1N1-Nluc. The pH1N1-Nluc replicates efficiently\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      , with growth kinetics and plaque morphology comparable to wild-type pH1N1 (pH1N1-WT). We used this pH1N1-Nluc to demonstrate its ability to effectively identify neutralizing monoclonal antibodies and antivirals, with neutralization and inhibition results comparable to pH1N1-WT. In mice, pH1N1-Nluc was able to induce similar body weight loss and mortality, and viral titers comparable to pH1N1-WT, results that were also recapitulated in a ferret model of IAV infection. Using IVIS, pH1N1-Nluc enabled non-invasive, real-time tracking of viral infection\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                      following infection of mice with viral titers in tissues comparable to pH1N1-WT. The flexibility of this approach was further demonstrated by the generation of a Nluc-expressing recombinant A\/Puerto Rico\/8\/1934 H1N1 (PR8-Nluc). Altogether, our results demonstrate that Nluc-expressing recombinant IAVs represent a valuable tool for\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      studies, including the identification of antivirals and\/or neutralizing antibodies, and to assess the protective efficacy of vaccines.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Influenza A viruses (IAVs) pose a threat to human and animal health. Mechanisms that control IAV replication and pathogenesis are incompletely understood due to the lack of experimental approaches to visualize and quantify viral dynamics in real time. The use of replication-competent fluorescent-expressing IAV\n                        <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                        has been challenging because such viruses typically have reduced replication fitness and are not suited for imaging of entire animals. Herein, we developed replication-competent recombinant IAV-expressing nanoluciferase (Nluc) that can be used to visualize viral infection in living animals. Infection with Nluc-expressing IAV could be monitored in real time using\n                        <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                        imaging systems. Importantly, the Nluc reporter overcomes several shortcomings of fluorescent proteins and provides a new and sensitive tool to interrogate viral dynamics and immune responses\n                        <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                        and\n                        <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                        . This technology can be applied to advance studies and accelerate the development of new prophylactics and therapeutics against IAV.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02150-25","type":"journal-article","created":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T13:01:16Z","timestamp":1759928476000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Bioluminescent reporter influenza A viruses to track viral infections"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8773-6349","authenticated-orcid":false,"given":"Ramya S.","family":"Barre","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00wbskb04","id-type":"ROR","asserted-by":"publisher"}],"name":"Texas Biomedical Research Institute","place":["San Antonio, USA"]},{"id":[{"id":"https:\/\/ror.org\/02f6dcw23","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Texas Health Sciences Center at San Antonio","place":["San Antonio, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2878-5714","authenticated-orcid":false,"given":"Ahmed","family":"Mostafa","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00wbskb04","id-type":"ROR","asserted-by":"publisher"}],"name":"Texas Biomedical Research Institute","place":["San Antonio, USA"]},{"id":[{"id":"https:\/\/ror.org\/02n85j827","id-type":"ROR","asserted-by":"publisher"}],"name":"Center of Scientific Excellence for Influenza Viruses, National Research Centre","place":["Giza, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Chiem","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00wbskb04","id-type":"ROR","asserted-by":"publisher"}],"name":"Texas Biomedical Research Institute","place":["San Antonio, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca L.","family":"Pearl","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04a9tmd77","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Icahn School of Medicine at Mount Sinai","place":["New York, USA"]},{"id":[{"id":"https:\/\/ror.org\/04a9tmd77","id-type":"ROR","asserted-by":"publisher"}],"name":"Global Health Emerging Pathogens Institute, Icahn School of Medicine at Mount Sinai","place":["New York, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roy N.","family":"Platt","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00wbskb04","id-type":"ROR","asserted-by":"publisher"}],"name":"Texas Biomedical Research Institute","place":["San Antonio, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasija","family":"Cupic","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04a9tmd77","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, Icahn School of Medicine at Mount Sinai","place":["New York, USA"]},{"id":[{"id":"https:\/\/ror.org\/04a9tmd77","id-type":"ROR","asserted-by":"publisher"}],"name":"Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai","place":["New York, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy J. 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