{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T09:56:43Z","timestamp":1755597403655,"version":"3.41.2"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T00:00:00Z","timestamp":1671580800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T00:00:00Z","timestamp":1671580800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2022,12,21]]},"abstract":"<jats:p>Clinical management of solid organ transplant (SOT) patients requires the careful monitoring of immunosuppression and viral infection or reactivation. qPCR is the gold standard for the detection and quantification of very small amounts of viral DNA and allows for an early assessment of viral load kinetics. The tested NeuMoDx 96 platform provides faster results than the previously used RT-PCR workflows for CMV (Abbott m2000 and RealTime CMV assay) and EBV (LightCycler 480 II, Roche high pure extraction, and Altona RealStar EBV assay) DNA detection.<\/jats:p>","DOI":"10.1128\/spectrum.02157-22","type":"journal-article","created":{"date-parts":[[2022,11,7]],"date-time":"2022-11-07T14:03:08Z","timestamp":1667829788000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Performance Evaluation of the Fully Automated NeuMoDx RT-PCR Platform for the Quantification of CMV and EBV DNA in EDTA Plasma: Implications for Clinical Management and Establishment of a Conversion Formula"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5176-7154","authenticated-orcid":true,"given":"Anna Nele","family":"Herdina","sequence":"first","affiliation":[{"name":"Division of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franz","family":"Ratzinger","sequence":"additional","affiliation":[{"name":"IhrLabor, Medical Diagnostic Laboratories, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Breuer","sequence":"additional","affiliation":[{"name":"Division of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Schellnegger","sequence":"additional","affiliation":[{"name":"Division of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui Qiang","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Watkins-Riedel","sequence":"additional","affiliation":[{"name":"Division of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Perkmann-Nagele","sequence":"additional","affiliation":[{"name":"Division of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2157-0382","authenticated-orcid":true,"given":"Robert","family":"Strassl","sequence":"additional","affiliation":[{"name":"Division of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00908-17"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2000.0778"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.06.036"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.2034"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.47372\/ejua-ba.2022.2.159"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.7189\/jogh.10.010404"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00067-13"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00177"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000002191"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/ctr.13652"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/ctr.13512"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(19)30107-0"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiz601"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/ctr.12657"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-814515-9.00125-9"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biologicals.2016.04.005"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03292-15"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01056-18"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/tid.12773"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2016.07.001"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2018.11.012"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0238062"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.2.525-526.1997"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.3.533"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2019.05.007"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01287-11"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03435-14"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.3390\/v13081619"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2022.105122"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis212"},{"key":"e_1_3_3_32_2","volume-title":"Molecular in vitro diagnostic examinations\u2014specifications for pre-examination processes for venous whole blood\u2014part 3: isolated circulating cell free DNA from plasma.","author":"International Standards Organization","year":"2019","unstructured":"International Standards Organization. 2019. Molecular in vitro diagnostic examinations\u2014specifications for pre-examination processes for venous whole blood\u2014part 3: isolated circulating cell free DNA from plasma. ISO 20186-3:2019. International Standards Organization, Geneva, Switzerland."},{"key":"e_1_3_3_33_2","volume-title":"1st WHO international standard for human cytomegalovirus for nucleic acid amplification techniques, NIBSC code: 09\/162, instructions for use (version 6.0, dated 09\/10\/2014)","author":"National Institute for Biological Standards and Control","year":"2014","unstructured":"National Institute for Biological Standards and Control. 2014. 1st WHO international standard for human cytomegalovirus for nucleic acid amplification techniques, NIBSC code: 09\/162, instructions for use (version 6.0, dated 09\/10\/2014). National Institute for Biological Standards and Control, Hertfordshire, UK."},{"key":"e_1_3_3_34_2","volume-title":"1st WHO International standard for Epstein-Barr virus for nucleic acid amplification techniques, NIBSC code: 09\/260, instructions for use (version 4.0, dated 09\/10\/2014)","author":"National Institute for Biological Standards and Control","year":"2014","unstructured":"National Institute for Biological Standards and Control. 2014. 1st WHO International standard for Epstein-Barr virus for nucleic acid amplification techniques, NIBSC code: 09\/260, instructions for use (version 4.0, dated 09\/10\/2014). National Institute for Biological Standards and Control, Hertfordshire, UK."},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1515\/cclm.1983.21.11.709"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)90837-8"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.02157-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.02157-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T14:39:00Z","timestamp":1671633540000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.02157-22"}},"subtitle":[],"editor":[{"given":"Kileen L.","family":"Shier","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2022,12,21]]},"references-count":35,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,12,21]]}},"alternative-id":["10.1128\/spectrum.02157-22"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.02157-22","relation":{},"ISSN":["2165-0497"],"issn-type":[{"type":"electronic","value":"2165-0497"}],"subject":[],"published":{"date-parts":[[2022,12,21]]},"assertion":[{"value":"2022-06-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02157-22"}}