{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:27:30Z","timestamp":1792787250880,"version":"4.5.0"},"reference-count":67,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T00:00:00Z","timestamp":1697500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T00:00:00Z","timestamp":1697500800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"HHS | National Institutes of Health","award":["R01 AI155424"],"award-info":[{"award-number":["R01 AI155424"]}]},{"name":"HHS | National Institutes of Health","award":["P30 KD117469"],"award-info":[{"award-number":["P30 KD117469"]}]},{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"crossref","award":["STANTO19R0"],"award-info":[{"award-number":["STANTO19R0"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"crossref","award":["JEAN21F0"],"award-info":[{"award-number":["JEAN21F0"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2023,10,17]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Bacterial infections in the lungs of persons with cystic fibrosis are typically composed of multispecies biofilm-like communities, which modulate clinically relevant phenotypes that cannot be explained in the context of a single species culture. Most analyses to date provide a picture of the transcriptional responses of individual pathogens; however, there is relatively little data describing the transcriptional landscape of clinically relevant multispecies communities. Harnessing a previously described cystic fibrosis-relevant, polymicrobial community model consisting of\n                    <jats:italic toggle=\"yes\">Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus sanguinis,<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Prevotella melaninogenica<\/jats:italic>\n                    , we performed an RNA-Seq analysis on the biofilm population to elucidate the transcriptional profiles of the community grown in artificial sputum medium (ASM) as compared to growth in monoculture, without mucin, and in fresh medium supplemented with tobramycin. We provide evidence that, although the transcriptional profile of\n                    <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    is community agnostic, the transcriptomes of\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">S. sanguinis<\/jats:italic>\n                    are community aware. Furthermore,\n                    <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">P. melaninogenica<\/jats:italic>\n                    are transcriptionally sensitive to the presence of mucin in ASM, whereas\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">S. sanguinis<\/jats:italic>\n                    largely do not alter their transcriptional profiles in the presence of mucin when grown in a community. Only\n                    <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    shows a robust response to tobramycin. Genetic studies of mutants altered in community-specific growth provide complementary data regarding how these microbes adapt to a community context.\n                  <\/jats:p>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>Polymicrobial infections constitute the majority of infections in the cystic fibrosis (CF) airway, but their study has largely been neglected in a laboratory setting. Our lab previously reported a polymicrobial community that can help explain clinical outcomes in the lungs of persons with CF. Here, we obtained transcriptional profiles of the community versus monocultures to provide transcriptional information about how this model community responds to CF-related growth conditions and perturbations. Genetic studies provide complementary functional outputs to assess how the microbes adapt to life in a community.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02201-23","type":"journal-article","created":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T09:02:50Z","timestamp":1695978170000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Transcriptional profiling and genetic analysis of a cystic fibrosis airway-relevant model shows asymmetric responses to growth in a polymicrobial community"],"prefix":"10.1128","volume":"11","author":[{"given":"Christopher A.","family":"Kesthely","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth","place":["Hanover, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rendi R.","family":"Rogers","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth","place":["Hanover, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bassam","family":"El Hafi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth","place":["Hanover, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrice","family":"Jean-Pierre","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth","place":["Hanover, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2861-4392","authenticated-orcid":false,"given":"George A.","family":"O\u2019Toole","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth","place":["Hanover, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2475911"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2010.08.002"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1526-0550(02)00005-7"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2014.09.012"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1203\/01.PDR.0000100758.66805.CE"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1513\/AnnalsATS.201306-159MG"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.03067"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/318075"},{"key":"e_1_3_4_10_2","first-page":"787","article-title":"The relevance of the polymicrobial nature of airway infection in the acute and chronic management of patients with cystic fibrosis","volume":"10","author":"Sibley CD","year":"2009","unstructured":"Sibley CD , Parkins MD , Rabin HR , Surette MG . 2009. 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