{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T15:53:10Z","timestamp":1791561190776,"version":"4.3.4"},"reference-count":9,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T00:00:00Z","timestamp":1736208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T00:00:00Z","timestamp":1736208000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,1,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n Diagnosis of\n Bordetella pertussis<\/jats:italic>\n is made by using polymerase chain reaction (PCR) to detect insertion sequence 481 (IS\n 481<\/jats:italic>\n ). However, IS\n 481<\/jats:italic>\n is found in both\n B. pertussis<\/jats:italic>\n and\n Bordetella holmesii<\/jats:italic>\n . In a recent study, Cole et al. (Microbiol Spectr 12:e00783-24.\n https:\/\/doi.org\/10.1128\/spectrum.00783-24<\/jats:ext-link>\n ) compared the accuracy of assays performed at two different commercial laboratories that used IS\n 481<\/jats:italic>\n as a molecular target against a multiplex PCR assay performed at the Centers for Disease Control and Prevention (CDC) that can differentiate the different\n Bordetella<\/jats:italic>\n species. The results demonstrated that the overall agreement among the assays from the commercial laboratories and the CDC was 85%. Only a small percentage (1.4%) of specimens positive for IS\n 481<\/jats:italic>\n were identified as\n B. holmesii<\/jats:italic>\n . This study suggests that PCR tests using IS\n 481<\/jats:italic>\n as a target provide reliable diagnosis for pertussis. However, end users should recognize the limitations of PCR testing for pertussis including the inability to differentiate and monitor transmission of non-pertussis\n Bordetella<\/jats:italic>\n species.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1128\/spectrum.02210-24","type":"journal-article","created":{"date-parts":[[2024,12,9]],"date-time":"2024-12-09T14:02:11Z","timestamp":1733752931000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Do not let perfect be the enemy of good: the current reality of\n Bordetella<\/i>\n testing"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5439-4218","authenticated-orcid":false,"given":"Rebecca","family":"Yee","sequence":"first","affiliation":[{"name":"Department of Pathology, George Washington University School of Medicine and Health Sciences","place":["Washington, DC, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm7003a2"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.2.326-382.2005"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00612-12"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cll.2014.02.003"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00783-24"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.arcped.2023.10.012"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cll.2023.10.001"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ514"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01339-14"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.02210-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.02210-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T14:07:17Z","timestamp":1736258837000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.02210-24"}},"subtitle":[],"editor":[{"given":"Siu-Kei","family":"Chow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,1,7]]},"references-count":9,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1,7]]}},"alternative-id":["10.1128\/spectrum.02210-24"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.02210-24","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,7]]},"assertion":[{"value":"2024-09-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02210-24"}}