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This study aimed to characterize the genotypic and phenotypic diversity of\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              isolates and evaluate their virulence using the\n              <jats:italic>Galleria mellonella<\/jats:italic>\n              model. Biomass production, metabolic activity, capsule formation, and siderophore production were assessed in 27\n              <jats:italic>K<\/jats:italic>\n              .\n              <jats:italic>pneumoniae<\/jats:italic>\n              isolates from hospital-associated infections. Lethality curves were generated using the\n              <jats:italic>G. mellonella<\/jats:italic>\n              model, with survival monitored hourly from 16 to 48 hours. The most common sequence types (ST) identified were the high-risk clones ST307 (\n              <jats:italic>N<\/jats:italic>\n              = 10), ST512 (\n              <jats:italic>N<\/jats:italic>\n              = 8), ST101 (\n              <jats:italic>N<\/jats:italic>\n              = 7), and ST661 (\n              <jats:italic>N<\/jats:italic>\n              = 2). These STs were associated with distinct K-locus, including KL102, KL107, KL17, and KL39. Most isolates belonged to the O2afg locus (\n              <jats:italic>N<\/jats:italic>\n              = 18), with the\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              carbapenemase genotype detected in 96.3% of strains. None of the isolates were classified as hypervirulent. Phenotypically, ST661 exhibited the highest biomass production despite showing similar metabolic activity to other STs. A positive correlation was observed between biomass and siderophore production, while capsule production was inversely correlated with biomass. In the\n              <jats:italic>G. mellonella<\/jats:italic>\n              model, ST661 demonstrated the highest virulence, resulting in 100% mortality by 48 hours, compared to survival rates of 21.4% for ST101, 38.0% for ST307, and 31.2% for ST512. These findings underscore the pathogenic potential of ST661 isolates with enhanced biofilm production. The\n              <jats:italic>G. mellonella<\/jats:italic>\n              model may serve as an effective\n              <jats:italic>in vivo<\/jats:italic>\n              system for evaluating the virulence of emerging\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              lineages.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                We demonstrate that the\n                <jats:italic>Galleria mellonella<\/jats:italic>\n                model is a useful tool to analyze the virulence of carbapenem-resistant\n                <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                strains. Our findings highlight the pathogenicity of carbapenem-resistant\n                <jats:italic>K pneumoniae<\/jats:italic>\n                isolates, particularly the role of the ST661 that, despite being a rare lineage, harbors the blaVIM gene and is associated with high biofilm production and the highest mortality rates.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.02215-24","type":"journal-article","created":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T14:03:52Z","timestamp":1736777032000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Virulence profile of carbapenem-resistant\n            <i>Klebsiella pneumoniae<\/i>\n            strains by an\n            <i>in vivo<\/i>\n            model of\n            <i>Galleria mellonella<\/i>"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7607-5848","authenticated-orcid":true,"given":"Mar\u00eda","family":"Guembe","sequence":"first","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Mara\u00f1\u00f3n","place":["Madrid, Spain"]},{"name":"IiSGM","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rama","family":"Hafian","sequence":"additional","affiliation":[{"name":"University of Alcala","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"D\u00edaz-Navarro","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Mara\u00f1\u00f3n","place":["Madrid, Spain"]},{"name":"IiSGM","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s","family":"Visedo","sequence":"additional","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Mara\u00f1\u00f3n","place":["Madrid, Spain"]},{"name":"IiSGM","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavio","family":"De Maio","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS","place":["Rome, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fulvia","family":"Pimpinelli","sequence":"additional","affiliation":[{"name":"Microbiology and Virology, San Gallicano Dermatological Institute, IRCCS","place":["Rome, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5092-0639","authenticated-orcid":true,"given":"Ilaria","family":"Cavallo","sequence":"additional","affiliation":[{"name":"Microbiology and Virology, San Gallicano Dermatological Institute, IRCCS","place":["Rome, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro","family":"Truglio","sequence":"additional","affiliation":[{"name":"Microbiology and Virology, San Gallicano Dermatological Institute, IRCCS","place":["Rome, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3552-1055","authenticated-orcid":true,"given":"Francesca","family":"Sivori","sequence":"additional","affiliation":[{"name":"Microbiology and Virology, San Gallicano Dermatological Institute, IRCCS","place":["Rome, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6295-6006","authenticated-orcid":true,"given":"Enea Gino","family":"Di Domenico","sequence":"additional","affiliation":[{"name":"Microbiology and Virology, San Gallicano Dermatological Institute, IRCCS","place":["Rome, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","volume-title":"Treasure Island (FL) ineligible companies. disclosure: Adam Dawson declares no relevant financial relationships with ineligible companies","author":"Ashurst JV","year":"2024","unstructured":"Ashurst JV, DawsonA. 2024. 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