{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T21:13:38Z","timestamp":1788642818323,"version":"build-2803163510"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,2,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The global dissemination of multidrug-resistant\n                      <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                      (MDR-KP) poses a critical One Health challenge. This study investigated the antimicrobial resistance (AMR) profiles, virulence determinants, and zoonotic transmission risks of\n                      <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                      isolates from humans (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 10) and animals (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 42), including bovine, porcine, and avian sources (ducks), in Central China. Whole-genome sequencing and phenotypic assays revealed a high prevalence of multidrug resistance (MDR) (92.3%, 48\/52) among\n                      <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                      isolates, with host-specific resistance rates: 100% in isolates from bovine and ducks, 90.9% in porcine isolates, and 70% in human isolates. Phylogenetic analysis revealed clustering of animal-derived sequence types (ST35, ST101, and ST592) with human clinical isolates, indicating potential cross-species transmission. No convergence of high virulence and MDR was observed. A representative ST11-KL64 strain (KP-HB21H30845) exhibited low virulence despite harboring canonical virulence gene clusters (\n                      <jats:italic toggle=\"yes\">iuc<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">rmpA<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">iroN<\/jats:italic>\n                      ). Genetic characterization identified a 45-bp deletion within the\n                      <jats:italic toggle=\"yes\">rmpA<\/jats:italic>\n                      promoter region, located within an ISKpn26-formed composite transposon on plasmid pKP845_1, which likely disrupts gene expression. Adjacent to\n                      <jats:italic toggle=\"yes\">iroN<\/jats:italic>\n                      genes positioned near ISKpn26 insertion sites may face transcriptional interference, contributing to attenuated virulence phenotypes. The plasmid-borne existence of AMR determinants (\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>KPC-2<\/jats:sub>\n                      and\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>CTX-M-65<\/jats:sub>\n                      ) and virulence loci (\n                      <jats:italic toggle=\"yes\">iuc<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">ybt<\/jats:italic>\n                      on ICEKp) highlights the emerging risk of MDR hypervirulent\n                      <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                      (MDR-hvKP). These findings underscore the urgent need for integrated surveillance and antimicrobial stewardship across human-animal interfaces to mitigate MDR-KP dissemination in both veterinary and clinical environments.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      MDR\n                      <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                      strains (ST35, ST101, and ST592) from animal hosts show genomic linkage to clinical human isolates, signaling interspecies transmission risks. The attenuated virulence of the ST11-KL64 is attributed to ISKpn26-mediated suppression of\n                      <jats:italic toggle=\"yes\">rmpA<\/jats:italic>\n                      expression, a key regulator of hypervirulence in\n                      <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02269-25","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T12:21:08Z","timestamp":1765974068000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Antimicrobial resistance, virulence defects, and evolutionary dynamics of multidrug-resistant\n                    <i>Klebsiella pneumoniae<\/i>\n                    from human and animal hosts in Central China"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3861-7850","authenticated-orcid":true,"given":"Wen","family":"Sun","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/023b72294","id-type":"ROR","asserted-by":"publisher"}],"name":"National Reference Laboratory of Veterinary Drug Residues and MOA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoman","family":"Zhu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/023b72294","id-type":"ROR","asserted-by":"publisher"}],"name":"National Reference Laboratory of Veterinary Drug Residues and MOA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomin","family":"Pang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/023b72294","id-type":"ROR","asserted-by":"publisher"}],"name":"National Reference Laboratory of Veterinary Drug Residues and MOA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyun","family":"Wu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/023b72294","id-type":"ROR","asserted-by":"publisher"}],"name":"National Reference Laboratory of Veterinary Drug Residues and MOA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University","place":["Wuhan, China"]},{"name":"Anlu Animal Disease Prevention and Control Center","place":["Anlu, 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