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Prescription of antibiotics to treat these infections is essential to maintain a higher quality of life and increase life expectancy. Determination of antibiotic susceptibility (ABS) is done by culture-dependent, phenotypic methods. These procedures take several days, while timely intervention is key. The analysis of antibiotic resistance genes by use of shotgun metagenomics might offer a time-sensitive alternative. Twenty people with CF with a homozygous Phe508del mutation provided 68 sputum samples during different visits over a period of roughly a year. After shotgun sequencing, the samples were analyzed using the deep learning tool deepARG. These results were compared with the results from routine ABS testing. The performance was determined by area under the curve-receiver operating characteristics (AUCROC) and sensitivity. Significant results were obtained for the following antibiotic classes: aminoglycoside (AUCROC = 0.81 [95% CI: 0.67\u20130.95, sensitivity = 73%]), cephalosporin (AUCROC = 0.70 [95% CI: 0.54\u20130.86, sensitivity = 95%]), and fluoroquinolone (AUCROC = 0.73 [95% CI: 0.56\u20130.89, sensitivity = 88%]). For other antibiotic classes, results were not significant. Using antibiotic class-specific cut-offs for positive reads of ARGs, a metagenomics approach potentially offers a culture-independent and more time-efficient manner to predict ABS for commonly prescribed antibiotic classes for sputum samples of adult people with CF. The use of metagenomics and artificial intelligence in clinical care will add to more personalized care for people with CF as well as better antibiotic stewardship.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>Damage induced by lung infections in people with cystic fibrosis (CF) is the leading factor to the mortality and morbidity of CF. To treat bacterial infections, people with CF are prescribed antibiotics. Routine antibiotic susceptibility (ABS) testing relies on culture-dependent, phenotypic techniques. These take several days up to more than a week, while timely intervention is key. To bridge this time gap, physicians in CF care use patient history of ABS data to start antibiotics, with risk of resistance to it. This pilot study explores a time saving alternative: the possibility to predict antibiotic resistance genes using shotgun metagenomics and artificial intelligence. By quicker prediction of ABS, people with CF can receive more adequate care, which results in the possible prevention of chronic infections and contributes to antibiotic stewardship.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02299-24","type":"journal-article","created":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T14:16:08Z","timestamp":1762352168000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Metagenomics approach to predict antibiotic resistance genes in sputum samples of adult people with cystic fibrosis: a pilot study"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8966-6185","authenticated-orcid":true,"given":"Sonja","family":"van Scheijen","sequence":"first","affiliation":[{"name":"Department of Pulmonary Medicine, Amsterdam UMC, University of Amsterdam","place":["Amsterdam, the Netherlands"]},{"id":[{"id":"https:\/\/ror.org\/008xxew50","id-type":"ROR","asserted-by":"publisher"}],"name":"Innovations in Human Health and Life Sciences, Department of Chemistry and Pharmaceutical Sciences, Vrije Universiteit Amsterdam","place":["Amsterdam, the Netherlands"]},{"name":"Amsterdam Institute for Immunology and Infectious diseases, Infectious diseases","place":["Amsterdam, the Netherlands"]},{"id":[{"id":"https:\/\/ror.org\/04gbbq803","id-type":"ROR","asserted-by":"publisher"}],"name":"Amsterdam Public Health, Personalized Medicine","place":["Amsterdam, the Netherlands"]},{"id":[{"id":"https:\/\/ror.org\/008xxew50","id-type":"ROR","asserted-by":"publisher"}],"name":"AIMMS Molecular Research Accelerator, Faculty of Science, Vrije Universiteit Amsterdam","place":["Amsterdam, the Netherlands"]},{"name":"Department of Pediatric Pulmonology and Allergy, Emma Children's Hospital, Amsterdam UMC","place":["Amsterdam, the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4533-486X","authenticated-orcid":false,"given":"Anne H.","family":"Neerincx","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine, Amsterdam UMC, University of Amsterdam","place":["Amsterdam, the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7146-1398","authenticated-orcid":false,"given":"Els J. 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