{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T05:29:05Z","timestamp":1785389345671,"version":"3.55.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T00:00:00Z","timestamp":1741046400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T00:00:00Z","timestamp":1741046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Ministry of Health-Singapore","award":["MOH-000645"],"award-info":[{"award-number":["MOH-000645"]}]},{"name":"Ministry of Health-Singapore","award":["NMRC\/CBRG\/0086\/2015"],"award-info":[{"award-number":["NMRC\/CBRG\/0086\/2015"]}]},{"name":"Department of Education and Training | Australian Research Council","award":["DP210100673"],"award-info":[{"award-number":["DP210100673"]}]},{"name":"DHAC | National Health and Medical Research Council","award":["GNT1143638"],"award-info":[{"award-number":["GNT1143638"]}]},{"name":"Ministry of Education-Singapore","award":["MOE2014-T2-2-124"],"award-info":[{"award-number":["MOE2014-T2-2-124"]}]},{"name":"Ministry of Education-Singapore","award":["RCE - SCELSE"],"award-info":[{"award-number":["RCE - SCELSE"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,3,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Enterococcus faecalis<\/jats:italic>\n              sortase-assembled endocarditis and biofilm-associated pili (Ebp) are virulence factors implicated in enterococcal biofilm-associated infections and gastrointestinal colonization. We previously showed that\n              <jats:italic>E. faecalis<\/jats:italic>\n              biofilm metabolism is influenced by extracellular electron transfer (EET) under iron-rich conditions, raising the question of whether Ebp pili also play a role in EET. Here, we report a novel role of Ebp pili in\n              <jats:italic>E. faecalis<\/jats:italic>\n              adhesion to the iron oxides magnetite, goethite, and hematite, where the EbpA tip adhesin contributes to this interaction. Adhesion by Ebp pili is conditionally important for EET to iron oxides, as pilus mutants are attenuated in EET under non-static growth conditions. In alignment with the established role of EET in redox homeostasis, we find that EET to ferricyanide supports\n              <jats:italic>E. faecalis<\/jats:italic>\n              anaerobic growth on glycerol. Furthermore, in an antibiotic-treated mouse gastrointestinal colonization model, we show that\n              <jats:italic>E. faecalis<\/jats:italic>\n              mutants deficient in EET poorly colonize the intestinal niche. Taken together, our findings suggest that Ebp pili can influence\n              <jats:italic>E. faecalis<\/jats:italic>\n              metabolic fitness by promoting EET to iron oxides, raising new questions about how Ebp pili shape\n              <jats:italic>E. faecalis<\/jats:italic>\n              interactions with environmental ecosystems. Additionally, the important role of EET in\n              <jats:italic>E. faecalis<\/jats:italic>\n              colonization of the dysbiotic gastrointestinal environment highlights the need for further inquiry into how EET contributes to\n              <jats:italic>E. faecalis<\/jats:italic>\n              microbial pathogenesis.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              In this study, we explored the interplay between extracellular electron transfer (EET) and an\n              <jats:italic>Enterococcus faecalis<\/jats:italic>\n              biofilm factor, the endocarditis and biofilm-associated pili (Ebp). We demonstrate that Ebp pili have a novel role in adhesion to iron oxides, which consequently promotes EET to iron oxides under non-static conditions. Along with our findings that\n              <jats:italic>E. faecalis<\/jats:italic>\n              EET can be coupled to anaerobic cell growth, our results point to a potential ecological role of Ebp pili in natural environments, outside of its established function in adhesion to host ligands. We provide the first evidence of the contribution of EET to\n              <jats:italic>E. faecalis<\/jats:italic>\n              colonization of the antibiotic-treated murine intestinal niche, which adds to the limited experimental evidence linking EET and microbial pathogenesis, as well as highlights the need for further studies of EET in bacterial pathogens.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.02337-24","type":"journal-article","created":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T14:06:07Z","timestamp":1738677967000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Role of sortase-assembled Ebp pili in\n            <i>Enterococcus faecalis<\/i>\n            adhesion to iron oxides and its impact on extracellular electron transfer"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0928-4786","authenticated-orcid":false,"given":"Foo Kiong","family":"Ho","sequence":"first","affiliation":[{"name":"Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University","place":["Singapore, Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0568-8628","authenticated-orcid":true,"given":"Ling Ning","family":"Lam","sequence":"additional","affiliation":[{"name":"Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University","place":["Singapore, Singapore"]},{"name":"School of Biological Sciences, Nanyang Technological University","place":["Singapore, Singapore"]},{"name":"Department of Oral Biology, College of Dentistry, University of Florida","place":["Gainesville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artur","family":"Matysik","sequence":"additional","affiliation":[{"name":"Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University","place":["Singapore, Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas Dean","family":"Watts","sequence":"additional","affiliation":[{"name":"Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University","place":["Singapore, Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun Jie","family":"Wong","sequence":"additional","affiliation":[{"name":"Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University","place":["Singapore, Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelvin Kian Long","family":"Chong","sequence":"additional","affiliation":[{"name":"Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University","place":["Singapore, Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei Yi","family":"Choo","sequence":"additional","affiliation":[{"name":"Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University","place":["Singapore, Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joe","family":"Tolar","sequence":"additional","affiliation":[{"name":"Department of BioSciences, Biomolecular Engineering Rice University","place":["Houston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pui Man","family":"Low","sequence":"additional","affiliation":[{"name":"Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University","place":["Singapore, Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi Sheng","family":"Chua","sequence":"additional","affiliation":[{"name":"Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University","place":["Singapore, Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason J.","family":"Paxman","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, La Trobe University","place":["Bundoora, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3469-7988","authenticated-orcid":true,"given":"Bego\u00f1a","family":"Heras","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, La Trobe University","place":["Bundoora, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico","family":"Marsili","sequence":"additional","affiliation":[{"name":"Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological 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