{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T17:37:13Z","timestamp":1787593033135,"version":"build-2736575974"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n Klebsiella pneumoniae<\/jats:italic>\n ST147 has emerged as a successful clone able to efficiently disseminate a number of carbapenemases, including\n bla<\/jats:italic>\n KPC<\/jats:sub>\n . This study compared a representative\n bla<\/jats:italic>\n KPC-3<\/jats:sub>\n -carrying plasmid of an ST147 clone with plasmids that usually harbor\n bla<\/jats:italic>\n KPC-3<\/jats:sub>\n belonging to the high-risk clone ST512. Five clinical isolates of KPC-3-producing\n K. pneumoniae<\/jats:italic>\n belonging to ST147 isolated in Southern Spain were analyzed. The first ST147 isolate detected was compared with two previous isolates of\n K. pneumoniae<\/jats:italic>\n ST512\/KPC-3 and KPC-3-encoding plasmid pKpQIL as reference plasmid. Microdilution, disk diffusion, \u03b2-CARBA test, and NG-Test CARBA 5 were used for antimicrobial susceptibility analysis and phenotypic characterization of the isolates according to EUCAST guidelines. Molecular characterization was performed using pulsed-field gel electrophoresis (PFGE)-\n Xba<\/jats:italic>\n I, sequenced (by Illumina and Oxford Nanopore) and annotated with open-source databases (CGE tools and RAST). Plasmid incompatibility groups were analyzed using PlasmidFinder; BRIG was used to compare the\n bla<\/jats:italic>\n KPC-3<\/jats:sub>\n -harboring plasmids from three of the selected clinical isolates with each other and with pKpQIL. Clinical isolates of ST147\/KPC-3 showed resistance to \u03b2-lactams, fluoroquinolones, and aminoglycosides. Clinical isolates ST147\/KPC-3 and ST512\/KPC-3 had identical PFGE patterns in each clone. Plasmid replicon analysis showed a plasmid with the formula IncFII (K2:A-:B-) in the ST512 isolates, identical to pKpQIL; the ST147 isolates harbored IncFII (K1:A-:B-), although the genetic environment of\n bla<\/jats:italic>\n KPC-3<\/jats:sub>\n was the same as pKpQIL, which is Tn\n 4401<\/jats:italic>\n _IS\n Kpn7<\/jats:italic>\n upstream of\n bla<\/jats:italic>\n KPC-3<\/jats:sub>\n and IS\n Kpn6<\/jats:italic>\n downstream of\n bla<\/jats:italic>\n KPC-3<\/jats:sub>\n . Plasmids harboring\n bla<\/jats:italic>\n KPC-3<\/jats:sub>\n in ST147 and ST512 are different, although differences are subtle. The genetic environment of\n bla<\/jats:italic>\n KPC-3<\/jats:sub>\n in the different sequence types is very conservative and identical to pKpQIL.\n <\/jats:p>\n \n IMPORTANCE<\/jats:title>\n \n The successful spread of\n bla<\/jats:italic>\n KPC<\/jats:sub>\n is primarily due to the dissemination of\n K. pneumoniae<\/jats:italic>\n isolates belonging to high-risk clonal complex 258 (ST258, ST11, and ST512); however, new clones are emerging globally as the ST147 clone. In this study, we compare the genetic environment of a representative\n bla<\/jats:italic>\n KPC-3<\/jats:sub>\n -carrying plasmid of an ST147 clone with plasmids that usually harbor\n bla<\/jats:italic>\n KPC-3<\/jats:sub>\n belonging to the high-risk clone ST512. Plasmids harboring\n bla<\/jats:italic>\n KPC-3<\/jats:sub>\n detected in ST512 and ST147 were different; however, the close genetic environments of\n bla<\/jats:italic>\n KPC-3<\/jats:sub>\n in the different plasmids (ST147 and ST512) remained conserved.\n <\/jats:p>\n <\/jats:sec>\n <\/jats:sec>","DOI":"10.1128\/spectrum.02338-24","type":"journal-article","created":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T09:00:43Z","timestamp":1747990843000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Characterization of a\n bla<\/i>\n KPC-3<\/sub>\n -carrying plasmid in a clinical isolate of\n Klebsiella pneumoniae<\/i>\n belonging to the emerging successful clone ST147"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7555-2924","authenticated-orcid":true,"given":"Esther","family":"Recacha","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/016p83279","id-type":"ROR","asserted-by":"publisher"}],"name":"Unidad de Gesti\u00f3n Cl\u00ednica de Enfermedades Infecciosas y Microbiolog\u00eda, Hospital Universitario Virgen Macarena","place":["Sevilla, Spain"]},{"name":"Instituto de Biomedicina de Sevilla\/Hospital Virgen Macarena, Universidad de Sevilla\/CSIC","place":["Sevilla, Spain"]},{"id":[{"id":"https:\/\/ror.org\/00ca2c886","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2961-2620","authenticated-orcid":true,"given":"Mercedes","family":"Delgado-Valverde","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/016p83279","id-type":"ROR","asserted-by":"publisher"}],"name":"Unidad de Gesti\u00f3n Cl\u00ednica de Enfermedades Infecciosas y Microbiolog\u00eda, Hospital Universitario Virgen Macarena","place":["Sevilla, Spain"]},{"name":"Instituto de Biomedicina de Sevilla\/Hospital Virgen Macarena, Universidad de Sevilla\/CSIC","place":["Sevilla, Spain"]},{"id":[{"id":"https:\/\/ror.org\/00ca2c886","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1633-4294","authenticated-orcid":false,"given":"Marina R.","family":"Pulido","sequence":"additional","affiliation":[{"name":"Instituto de Biomedicina de Sevilla\/Hospital Virgen Macarena, Universidad de Sevilla\/CSIC","place":["Sevilla, Spain"]},{"id":[{"id":"https:\/\/ror.org\/00ca2c886","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III","place":["Madrid, Spain"]},{"id":[{"id":"https:\/\/ror.org\/03yxnpp24","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Microbiolog\u00eda, Facultad de Medicina, Universidad de Sevilla","place":["Sevilla, 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Spain"]},{"name":"Instituto de Biomedicina de Sevilla\/Hospital Virgen Macarena, Universidad de Sevilla\/CSIC","place":["Sevilla, Spain"]},{"id":[{"id":"https:\/\/ror.org\/00ca2c886","id-type":"ROR","asserted-by":"publisher"}],"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III","place":["Madrid, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Manuel","family":"S\u00e1nchez-Calvo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04v0snf24","id-type":"ROR","asserted-by":"publisher"}],"name":"Unit of Infectious Diseases and Clinical Microbiology, Jerez de la Frontera University Hospital","place":["Jerez de la Frontera, 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