{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T01:01:11Z","timestamp":1782781271605,"version":"3.54.5"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"New Frontiers in Research Funds Exploration Grant","award":["NFRFE-2021- 00398"],"award-info":[{"award-number":["NFRFE-2021- 00398"]}]},{"DOI":"10.13039\/501100001804","name":"Canada Research Chairs","doi-asserted-by":"crossref","award":["CRC-2019- 00115"],"award-info":[{"award-number":["CRC-2019- 00115"]}],"id":[{"id":"10.13039\/501100001804","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Canadian Government | Canadian Institutes of Health Research","award":["CGS-M"],"award-info":[{"award-number":["CGS-M"]}]},{"DOI":"10.13039\/501100004489","name":"Mitacs","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004489","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,12,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Methicillin-resistant\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      (MRSA) is the leading cause of wound infections, often progressing into serious invasive bloodstream infections. MRSA disproportionately affects Indigenous peoples in Canada with higher rates of skin and wound infections, an example of persistent gaps in health outcomes between Indigenous and non-Indigenous peoples precipitated by the legacy of colonialism. Conversely, Indigenous peoples have long used natural remedies for infections and other diseases; however, their knowledge was rarely considered for modern medicine. The stagnant antibiotic discovery pipeline and alarming rise of resistance to current antibiotics prompted us to turn to Indigenous medicine as an untapped source of antimicrobials. As such, we collected and prepared 85 extracts of medicinal plants of value to Indigenous peoples spanning the Canadian Prairies. We explored the antimicrobial potential of these extracts against MRSA under wound infection-mimetic conditions compared with culture media typically used to study bacterial antibiotic responses and biofilms but not adequately representative of infection sites. We identified extracts with MRSA growth inhibitory [\n                      <jats:italic>e.g.<\/jats:italic>\n                      , bergamot, dock, gaillardia, and dandelion extracts] and biofilm prevention and eradication [\n                      <jats:italic>e.g.<\/jats:italic>\n                      , gumweed extracts] activities. Extracts, including those of chokecherry, hoary puccoon, and Northern bedstraw, were only active under wound infection-mimetic conditions, highlighting the benefit of antibiotic discovery under host-relevant conditions. Testing growth inhibitory extracts against an\n                      <jats:italic>S. aureus<\/jats:italic>\n                      cross-resistance platform suggested that they act through mechanisms likely distinct from known antibiotic classes. Together, through an interdisciplinary partnership leveraging Western approaches and traditional Indigenous knowledge, we identified plant extracts with promising antimicrobial potential for drug-resistant MRSA wound infections.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>We explored the antimicrobial potential of traditional Indigenous remedies against MRSA under wound infection-mimetic conditions. We chose to tackle MRSA wound infections because they constitute an Indigenous health priority, ensuring mutual benefits and reciprocity, which are important principles in partnerships between Indigenous and non-Indigenous researchers. Our partnerships strive to serve as steps towards reconciliation with Indigenous peoples in Canada and a roadmap inspiring similar interdisciplinary collaborations to tackle other healthcare priorities. We identified extracts with promising antibacterial growth inhibitory, biofilm prevention, and eradication activities against MRSA. The antimicrobial potential of some extracts was only observed under wound infection-mimetic conditions, a proof-of-concept that screening under infection-mimetic conditions reveals novel activity undetected under standard conditions. The natural product antimicrobial extracts discovered herein warrant further investigation into their mode of action and chemical composition; they may address the dire need for new antimicrobial and anti-biofilm activity to counter the AMR crisis.<\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02341-24","type":"journal-article","created":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T09:01:55Z","timestamp":1731402115000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["The antimicrobial potential of traditional remedies of Indigenous peoples from Canada against MRSA planktonic and biofilm bacteria in wound infection mimetic conditions"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-7008-6846","authenticated-orcid":false,"given":"Colin D.","family":"Rieger","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of Science, University of Regina","place":["Regina, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed M.","family":"Soliman","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of Science, University of Regina","place":["Regina, Canada"]},{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy, Kafr-Elsheikh University","place":["Kafr El-Sheikh, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kateryna","family":"Kaplia","sequence":"additional","affiliation":[{"name":"Department of Indigenous Knowledge and Science, Faculty of Science, First Nations University of Canada","place":["Regina, Canada"]},{"name":"Kingston University, Faculty of Health, Science, Social Care and Education","place":["Kingston, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilrup","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Department of Indigenous Knowledge and Science, Faculty of Science, First Nations University of Canada","place":["Regina, Canada"]},{"name":"IISER Kolkata","place":["West Bengal, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexa Cervantes","family":"Lopez","sequence":"additional","affiliation":[{"name":"Department of Indigenous Knowledge and Science, Faculty of Science, First Nations University of Canada","place":["Regina, Canada"]},{"name":"Instituto Tecnol\u00f3gico y de Estudios Superiores de Monterrey","place":["Monterrey, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surya Arcot","family":"Venkatesan","sequence":"additional","affiliation":[{"name":"Department of Indigenous Knowledge and Science, Faculty of Science, First Nations University of Canada","place":["Regina, Canada"]},{"name":"Engineering and Technology, Rajalakshmi Engineering College","place":["Kanchipuram, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abraham Gildaro","family":"Guevara Flores","sequence":"additional","affiliation":[{"name":"Department of Indigenous Knowledge and Science, Faculty of Science, First Nations University of Canada","place":["Regina, Canada"]},{"name":"Department of Chemistry, Autonomous University of Nuevo Leon (Medicine College)","place":["San Nicol\u00e1s de los Garza, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matheus Ant\u00f4nio","family":"Filiol Belin","sequence":"additional","affiliation":[{"name":"Department of Indigenous Knowledge and Science, Faculty of Science, First Nations University of Canada","place":["Regina, Canada"]},{"name":"Universidade Estadual Paulista (UNESP), Campus de Botucatu. 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