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However, detection and isolation of these minor serogroups of STEC are challenging due to the lack of specific isolation methods. Here, we present a case of HUS in which STEC was not isolated by routine diagnostic tests for the major serotypes. Therefore, we tried a new diagnostic and isolation method that combines PCR screening, immunomagnetic bead separation, and serum agglutination tests and successfully isolated STEC O76. Subsequent genomic analyses of the STEC O76 isolates revealed that several isolates of this serogroup carrying\n            <jats:italic>stx2<\/jats:italic>\n            were related to severe infections. The complete genome of the HUS-derived isolates provided two important implications. First, using a complete genome as a reference in core genome single nucleotide polymorphism analysis leads to the highest resolution of the analysis. Second, the HUS-derived STEC O76:H7 possessed two copies of Stx2a prophages, and one of them showed a \u201cprophage integrating into prophage\u201d structure, as described in STEC O145:H28. These results demonstrate that our detection methods contribute to the diagnosis and isolation of minor serogroups of STECs and complete genomic analyses can illuminate the pathogenic potential of STECs.\n          <\/jats:p>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Hemolytic uremic syndrome (HUS) is a life-threatening disease caused by Shiga toxin-producing\n              <jats:italic>Escherichia coli<\/jats:italic>\n              (STEC) infection. The treatment approaches for STEC-mediated typical HUS and atypical HUS differ, underscoring the importance of rapid and accurate diagnosis. However, specific detection methods for STECs other than major serogroups, such as O157, O26, and O111, are limited. This study focuses on the utility of PCR-based O-serotyping, serum agglutination tests utilizing antibodies against the identified Og type, and isolation techniques employing antibody-conjugated immunomagnetic beads for STEC isolation. By employing these methods, we successfully isolated a STEC strain of a minor serotype, O76:H7, from a HUS patient.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.02355-23","type":"journal-article","created":{"date-parts":[[2023,12,4]],"date-time":"2023-12-04T14:01:28Z","timestamp":1701698488000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Combined usage of serodiagnosis and O antigen typing to isolate Shiga toxin-producing\n            <i>Escherichia coli<\/i>\n            O76:H7 from a hemolytic uremic syndrome case and genomic insights from the isolate"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4765-0989","authenticated-orcid":true,"given":"Kenichi","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Bacteriology I, National Institute of Infectious Diseases , Tokyo, 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