{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T16:27:31Z","timestamp":1789230451491,"version":"build-2803163510"},"reference-count":17,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,2,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The value of Fourier transform infrared (FTIR) spectroscopy as an investigation tool for outbreaks caused by\n                      <jats:italic toggle=\"yes\">Acinetobacter baumannii<\/jats:italic>\n                      remains inconclusive. Here, we used FTIR as a real-time tool to investigate a carbapenem-resistant\n                      <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                      (CRAB) outbreak and later compared the FTIR results to results of whole genome sequencing (WGS). We used single-nucleotide polymorphism (SNP) analysis to confirm the correct FTIR cutoff and validate FTIR clustering. The outbreak occurred mainly in a general intensive care unit of an Israeli tertiary care hospital and involved 22 patients detected between December 2022 and August 2023. Isolates from 21 patients and two environmental sources were analyzed by FTIR. Eleven representative isolates underwent WGS. We performed SNP analysis using the earliest isolate as the reference. FTIR revealed that the outbreak was comprised of three clusters and four singletons. FTIR results were concordant with WGS for the 11 sequenced isolates. FTIR had higher discriminatory power than multilocus sequence typing. Within-cluster SNP differences were up to 21 SNPs. This finding confirmed that the cutoff we chose to define FTIR clusters (0.252) was appropriate. FTIR uncovered that what appeared to be one CRAB outbreak was actually three parallel outbreaks. FTIR analysis of CRAB outbreaks yields results similar to WGS, but faster and at a lower cost. A SNP threshold of less than 21 SNPs is appropriate when analyzing an\n                      <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                      outbreak.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        This study demonstrated that Fourier transform infrared (FTIR) spectroscopy could effectively identify\n                        <jats:italic toggle=\"yes\">Acinetobacter baumannii<\/jats:italic>\n                        outbreaks. Serving as a practical and rapid alternative to whole genome sequencing, FTIR can significantly accelerate\n                        <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                        outbreak confirmation, resulting in effective infection control interventions.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02392-25","type":"journal-article","created":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T14:01:22Z","timestamp":1766152882000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Contribution of Fourier transform infrared spectroscopy for outbreak investigation of carbapenem-resistant\n                    <i>Acinetobacter baumannii<\/i>"],"prefix":"10.1128","volume":"14","author":[{"given":"Hadas","family":"Kon","sequence":"first","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control","place":["Tel Aviv, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2488-3720","authenticated-orcid":true,"given":"Mor N.","family":"Lurie-Weinberger","sequence":"additional","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control","place":["Tel Aviv, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dafna","family":"Chen","sequence":"additional","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control","place":["Tel Aviv, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hani","family":"Laderman","sequence":"additional","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control","place":["Tel Aviv, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Temkin","sequence":"additional","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control","place":["Tel Aviv, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Lomansov","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01fm87m50","id-type":"ROR","asserted-by":"publisher"}],"name":"Infection Control Unit, Rambam Health Care Campus","place":["Haifa, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibraheem","family":"Firan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01fm87m50","id-type":"ROR","asserted-by":"publisher"}],"name":"Infection Control Unit, Rambam Health Care Campus","place":["Haifa, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Worood","family":"Aboalhega","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01fm87m50","id-type":"ROR","asserted-by":"publisher"}],"name":"Infection Control Unit, Rambam Health Care Campus","place":["Haifa, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Raines","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01fm87m50","id-type":"ROR","asserted-by":"publisher"}],"name":"Infection Control Unit, Rambam Health Care Campus","place":["Haifa, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0679-3967","authenticated-orcid":true,"given":"Alona","family":"Keren-Paz","sequence":"additional","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control","place":["Tel Aviv, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yehuda","family":"Carmeli","sequence":"additional","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control","place":["Tel Aviv, Israel"]},{"id":[{"id":"https:\/\/ror.org\/04mhzgx49","id-type":"ROR","asserted-by":"publisher"}],"name":"Faculty of Medical and Health Sciences, Tel Aviv University","place":["Tel Aviv, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12363"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-6-130"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00055"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9030119"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.13709"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b05050"},{"key":"e_1_3_3_8_2","first-page":"29","article-title":"Correlation of strain classification with IR biotyper and molecular epidemiological method of Pseudomonas aeruginosa","volume":"31","author":"Oho M","year":"2021","unstructured":"Oho M, Nagasawa Z, Funashima Y, Ueda O, Watamabe S, Cui L, Miyamoto H, Sueoka E. 2021. Correlation of strain classification with IR biotyper and molecular epidemiological method of Pseudomonas aeruginosa. Rinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi 31:29\u201340.","journal-title":"Rinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1017\/ice.2022.109"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2020-0276"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00347-23"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.infpip.2021.100113"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s42770-022-00774-6"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2024.01.002"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01440"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-018-0343-z"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-025-01547-0"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000530"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.02392-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.02392-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:45:51Z","timestamp":1788223551000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.02392-25"}},"subtitle":[],"editor":[{"given":"Karen C.","family":"Carroll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,2,3]]},"references-count":17,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2,3]]}},"alternative-id":["10.1128\/spectrum.02392-25"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.02392-25","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,3]]},"assertion":[{"value":"2025-08-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02392-25"}}