{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T19:29:21Z","timestamp":1789500561541,"version":"build-2803163510"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Currently,\n                      <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      ) is one of the leading causes of death from infectious diseases worldwide. Our aim in this study was to investigate and compare the molecular epidemiology and antibiotic resistance of\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      colonizing the intestinal tract of pediatric inpatients and outpatients of different age groups. We analyzed stool samples from 1,300 patients at a children\u2019s hospital in Shenzhen, China. After culturing\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      , we used matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry to identify the strains. In addition, we performed antimicrobial susceptibility testing on isolated\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      strains as well as pheno- and genotypic characterization by PCR.\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      was detected in 104 out of 1,300 (8.0%) patients, including 20 out of 1,300 (1.5%) patients with methicillin-resistant\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      (MRSA). MRSA accounted for 19.2% of the\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      isolates. The resistance rates of\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      strains to penicillin, erythromycin, clindamycin, levofloxacin, and moxifloxacin were 83.7%, 34.6%, 31.7%, 3.9%, and 3.9%, respectively. None of the strains showed resistance to linezolid, daptomycin, tigecycline, vancomycin, or tetracycline. One hundred fou strains of\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      revealed that 49.0% (51\/104) harbored enterotoxin genes, and most enterotoxin-positive strains carried only one gene type (90.2%, 46\/51), while a minority carried two gene types (9.8%, 5\/51). Besides, a total of 29 sequence types (STs) were identified with the three most prevalent STs: ST45, ST188, and ST6, accounting for 12.5%, 12.5%, and 9.6%. We found that the molecular characteristics of\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      in intestinal colonization of children have regional differences. To provide a theoretical basis for the prevention and control of\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      infections, increased surveillance of local\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      resistance and molecular epidemiological characteristics is needed.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        This study assessed the clinical and molecular epidemiology of\n                        <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                        in pediatric patients at a children\u2019s hospital in Shenzhen, South China by means of screening stool samples for pheno- and genotypic characterization for carriage of\n                        <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                        . Of 1,300 fecal samples screened, 104 (8.0%) were positive for\n                        <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                        with 19.2% methicillin-resistant\n                        <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                        . The resistance rates of\n                        <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                        to penicillin, erythromycin, clindamycin, levofloxacin, and moxifloxacin were 83.7%, 34.6%, 31.7%, 3.9%, and 3.9%, respectively. None of the strains showed resistance to linezolid, daptomycin, tigecycline, vancomycin, or tetracycline. One hundred four strains of\n                        <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                        revealed that 49.0% (51\/104) harbored enterotoxin genes, and most enterotoxin-positive strains carried only one gene type (90.2%, 46\/51), while a minority carried two gene types (9.8%, 5\/51). Besides, a total of 29 sequence types (STs) were identified with the three most prevalent STs: ST45, ST188, and ST6, accounting for 12.5%, 12.5%, and 9.6%.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02394-24","type":"journal-article","created":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T09:00:51Z","timestamp":1747386051000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["A molecular comparative study of intestinal colonization with\n                    <i>Staphylococcus aureus<\/i>\n                    between pediatric inpatients and outpatients of different age groups"],"prefix":"10.1128","volume":"13","author":[{"given":"Shaoxiang","family":"Lin","sequence":"first","affiliation":[{"name":"Affiliated Shenzhen Women and Children's Hospital (Longgang) of Shantou University Medical College (Longgang District 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