{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T17:17:00Z","timestamp":1781889420074,"version":"3.54.5"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T00:00:00Z","timestamp":1738627200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T00:00:00Z","timestamp":1738627200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100017590","name":"Hubei University of Science and Technology","doi-asserted-by":"crossref","award":["BK202410"],"award-info":[{"award-number":["BK202410"]}],"id":[{"id":"10.13039\/501100017590","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100017590","name":"Hubei University of Science and Technology","doi-asserted-by":"crossref","award":["BK202332"],"award-info":[{"award-number":["BK202332"]}],"id":[{"id":"10.13039\/501100017590","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100017590","name":"Hubei University of Science and Technology","doi-asserted-by":"crossref","award":["2023T13"],"award-info":[{"award-number":["2023T13"]}],"id":[{"id":"10.13039\/501100017590","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,2,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Mercury pollution is a kind of heavy metal pollution with great harm and strong toxicity which exists worldwide. Some microorganisms can convert highly toxic methylmercury into inorganic mercury compounds with significantly reduced toxicity. This is an effective means of methylmercury pollution remediation. As a safe microorganism with great potential in the remediation of heavy metal pollution,\n              <jats:italic>Rhodotorula mucilaginosa<\/jats:italic>\n              has not been studied in the remediation of methylmercury. Here, a\n              <jats:italic>R. mucilaginosa<\/jats:italic>\n              strain Rm4 with high methylmercury resistance was obtained by wild-strain screening. Its minimal inhibitory concentration and minimum lethal concentration reached 3 and 6 mg\/L, respectively. At the same time, Rm4 can also degrade methylmercury. Unlike the traditional microbial methylmercury degradation pathways,\n              <jats:italic>R. mucilaginosa<\/jats:italic>\n              \u2019s genome does not encode the organomercury lyase gene MerB. However, transcriptomic analysis revealed that the glutathione reductase of\n              <jats:italic>R. mucilaginosa<\/jats:italic>\n              responds to the methylmercury degradation process. Structural domain analysis and molecular docking experiments suggest that the glutathione reductase of\n              <jats:italic>R. mucilaginosa<\/jats:italic>\n              has the potential to directly or indirectly participate in methylmercury degradation metabolism. Metabolic indicator tests of engineered strains overexpressing the glutathione reductase encoding gene also support this notion.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              The remediation of methylmercury pollution is crucial for environmental health. The ability of\n              <jats:italic>Rhodotorula mucilaginosa<\/jats:italic>\n              to resist and degrade methylmercury offers a new avenue for bioremediation efforts. Understanding the metabolic pathways involved, particularly the role of glutathione reductase, enhances our knowledge of how\n              <jats:italic>Rhodotorula mucilaginosa<\/jats:italic>\n              responds to methylmercury and opens up new possibilities for future research in bioremediation strategies.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.02395-24","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T14:00:35Z","timestamp":1737036035000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Glutathione reductase plays a role in the metabolism of methylmercury degradation in\n            <i>Rhodotorula mucilaginosa<\/i>"],"prefix":"10.1128","volume":"13","author":[{"given":"Yi","family":"Guo","sequence":"first","affiliation":[{"name":"School of Basic Medical Sciences, Xianning Medical College, Hubei University of Science and 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Laboratory of Diabetes and Angiopathy, Hubei University of Science and Technology","place":["Xianning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingjie","family":"Wei","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hubei University of Science and Technology","place":["Xianning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiling","family":"Tang","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Xianning Medical College, Hubei University of Science and Technology","place":["Xianning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8756-2464","authenticated-orcid":true,"given":"Surui","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Xianning Medical College, Hubei University of Science and Technology","place":["Xianning, 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