{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T21:26:40Z","timestamp":1792099600346,"version":"4.4.0"},"reference-count":14,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T00:00:00Z","timestamp":1741046400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T00:00:00Z","timestamp":1741046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Canadian Government | Natural Sciences and Engineering Research Council of Canada","award":["RGPIN-2019-07135"],"award-info":[{"award-number":["RGPIN-2019-07135"]}]},{"name":"Canadian Government | Natural Sciences and Engineering Research Council of Canada","award":["RGPIN-2022-04239"],"award-info":[{"award-number":["RGPIN-2022-04239"]}]},{"name":"Canadian Government | Canadian Institutes of Health Research","award":["Strategic Master's Award"],"award-info":[{"award-number":["Strategic Master's Award"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,3,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Antimicrobial resistance (AMR) is a global threat. The identification and characterization of novel resistance genes is integral to AMR surveillance. The\n              <jats:italic>erm<\/jats:italic>\n              (55) gene was originally identified through whole genome sequencing of macrolide-resistant strains of\n              <jats:italic>Mycobacterium chelonae<\/jats:italic>\n              . The gene was annotated as a ribosomal methyltransferase, but its role as a determinant of macrolide resistance was not formally demonstrated. Three\n              <jats:italic>erm<\/jats:italic>\n              (55) alleles have now been documented. The plasmid-borne\n              <jats:italic>erm<\/jats:italic>\n              (55)\n              <jats:sup>P<\/jats:sup>\n              , the transposon-associated\n              <jats:italic>erm<\/jats:italic>\n              (55)\n              <jats:sup>T<\/jats:sup>\n              , and the chromosomal encoded\n              <jats:italic>erm<\/jats:italic>\n              (55)\n              <jats:sup>C<\/jats:sup>\n              exhibit \u224882% amino acid sequence identity. Here, we confirm that, when expressed from plasmids in a macrolide-susceptible strain of\n              <jats:italic>Escherichia coli<\/jats:italic>\n              , all three\n              <jats:italic>erm<\/jats:italic>\n              (55) variants confer resistance to azithromycin and clarithromycin.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Macrolide antibiotics are often the only oral treatment option for infections with rapidly growing mycobacteria such as\n              <jats:italic>Mycobacterium abscessus<\/jats:italic>\n              and\n              <jats:italic>Mycobacterium chelonae<\/jats:italic>\n              . We previously identified three variants of a newly predicted macrolide resistance gene,\n              <jats:italic>erm<\/jats:italic>\n              (55), in\n              <jats:italic>M. chelonae<\/jats:italic>\n              , including the first case of a plasmid-mediated macrolide resistance in mycobacteria. The present study provides experimental evidence that the three\n              <jats:italic>erm<\/jats:italic>\n              (55) variants confer macrolide resistance and that each variant is unique in the degree to which it reduces susceptibility to clinically relevant macrolides.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.02397-24","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T14:02:36Z","timestamp":1737036156000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Macrolide resistance due to\n            <i>erm<\/i>\n            (55)"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3509-445X","authenticated-orcid":true,"given":"David C.","family":"Alexander","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, University of Manitoba","place":["Winnipeg, Canada"]},{"name":"Cadham Provincial Laboratory, Diagnostic Services, Shared Health","place":["Winnipeg, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tayah","family":"Farquhar","sequence":"additional","affiliation":[{"name":"Institute for Microbial Systems and Society, Faculty of Science, University of Regina","place":["Regina, Canada"]},{"name":"Department of Biology, Faculty of Science, University of Regina","place":["Regina, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua M. E.","family":"Adams","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of Science, University of Regina","place":["Regina, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8961-652X","authenticated-orcid":true,"given":"Danae M.","family":"Suchan","sequence":"additional","affiliation":[{"name":"Institute for Microbial Systems and Society, Faculty of Science, University of Regina","place":["Regina, Canada"]},{"name":"Department of Biology, Faculty of Science, University of Regina","place":["Regina, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8807-4466","authenticated-orcid":true,"given":"Sean D.","family":"Workman","sequence":"additional","affiliation":[{"name":"Institute for Microbial Systems and Society, Faculty of Science, University of Regina","place":["Regina, Canada"]},{"name":"Department of Biology, Faculty of Science, University of Regina","place":["Regina, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard J.","family":"Wallace Jr.","sequence":"additional","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, The University of Texas at Tyler Health Science Center, UT Tyler School of Medicine","place":["Tyler, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3091-1169","authenticated-orcid":true,"given":"Barbara A.","family":"Brown-Elliott","sequence":"additional","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, The University of Texas at Tyler Health Science Center, UT Tyler School of Medicine","place":["Tyler, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2829-5851","authenticated-orcid":true,"given":"Omar M.","family":"El-Halfawy","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Faculty of Science, University of Regina","place":["Regina, Canada"]},{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy, Alexandria University","place":["Alexandria, Egypt"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1560-8572","authenticated-orcid":true,"given":"Andrew D. S.","family":"Cameron","sequence":"additional","affiliation":[{"name":"Institute for Microbial Systems and Society, Faculty of Science, University of Regina","place":["Regina, Canada"]},{"name":"Department of Biology, Faculty of Science, University of Regina","place":["Regina, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13936"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.05030-11"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201003-0395OC"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a025395"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.12.2823"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00402-06"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01275-08"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00428-23"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90366-J"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01996-12"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01882-16"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2022.101571"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00032-14"},{"key":"e_1_3_4_15_2","volume-title":"Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard 9th ed., CLSI document M07-A9","author":"CLSI","year":"2012","unstructured":"CLSI. 2012. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard 9th ed., CLSI document M07-A9. Clinical and Laboratory Standards Institute, Wayne, PA."}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.02397-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.02397-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T14:05:55Z","timestamp":1741097155000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.02397-24"}},"subtitle":[],"editor":[{"given":"Siu-Kei","family":"Chow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,3,4]]},"references-count":14,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3,4]]}},"alternative-id":["10.1128\/spectrum.02397-24"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.02397-24","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,4]]},"assertion":[{"value":"2024-09-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02397-24"}}