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Macrolide still plays a significant role in treating infections by\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    . However, macrolide-resistant\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    (MRMP) was encountered, causing more severe or prolonged disease worldwide from the early 2000s. Nemonoxacin is a newly developed C-8-methoxy non-fluorinated quinolone having broad-spectrum activity against gram-positive, gram-negative, and atypical pathogens\n                    <jats:italic>in vitro<\/jats:italic>\n                    , as well as general safety and well tolerance\n                    <jats:italic>in vivo<\/jats:italic>\n                    . This study aimed to assess the killing kinetics of nemonoxacin and other antimicrobial agents against MRMP and macrolide susceptible\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    (MSMP) by static time-kill curves. In addition, the pharmacodynamic analyses of these drugs were determined by the sigmoid\n                    <jats:italic>E<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    model. The static time-kill curves indicated that nemonoxacin had good mycoplasmacidal activity against both MSMP and MRMP strains. The pharmacodynamic analysis demonstrated that the mycoplasmacidal effect of nemonoxacin was greater than those of moxifloxacin and levofloxacin based on the\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    , displaying a dualism of concentration dependency in low minimum inhibitory concentrations (MICs) and time dependency in high MICs. Therefore, nemonoxacin demonstrated efficacy against\n                    <jats:italic>M. pneumoniae in vitro<\/jats:italic>\n                    and is a potential candidate for clinical studies for assessing its therapeutic effects against\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    infections. The killing pattern or PK\/PD characteristics of nemonoxacin against\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    from\n                    <jats:italic>in vitro<\/jats:italic>\n                    data, animal studies, or clinical studies merit further evaluation for treating infections caused by the organism.\n                  <\/jats:p>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      This study first reported the\n                      <jats:italic>in vitro<\/jats:italic>\n                      effector kinetics of the new non-fluorinated quinolone, nemonoxacin, against macrolide-resistant\n                      <jats:italic>M. pneumoniae<\/jats:italic>\n                      (MRMP) and macrolide susceptible\n                      <jats:italic>M. pneumoniae<\/jats:italic>\n                      (MSMP) strains along with other antimicrobial agents. The time-kill assays and pharmacodynamic analysis showed that nemonoxacin has significant mycoplasmacidal activity against MRMP and MSMP. This study paves the road to establish appropriate dosing protocols of a new antimicrobial drug for children infected with\n                      <jats:italic>M. pneumoniae<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02431-23","type":"journal-article","created":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T09:02:30Z","timestamp":1700211750000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["<i>In vitro<\/i>\n                    pharmacodynamics of nemonoxacin and other antimicrobial agents against\n                    <i>Mycoplasma pneumoniae<\/i>"],"prefix":"10.1128","volume":"11","author":[{"given":"Na","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Antibiotics, Huashan Hospital, Fudan University , Shanghai, China"},{"name":"Key Laboratory of Clinical Pharmacology of Antibiotics , Shanghai, China"},{"name":"National Health Commission &amp; 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