{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:55:25Z","timestamp":1791294925126,"version":"4.1.0"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM122510"],"award-info":[{"award-number":["GM122510"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,10,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>It has been known for more than half a century that enzymes can polymerize to either activate or repress their catalytic functions. Using electron cryo-microscopy (cryo-EM), we serendipitously found that two commonly used enzymes in molecular microbiology research, alkaline phosphatase and proteinase K, can unexpectedly form filaments. While the physiological significance of fibrillation in alkaline phosphatase and proteinase K is unknown, the atomic models of these polymers show the ability of cryo-EM to discover and determine high-resolution structures of macromolecular assemblies before they have been characterized or purified.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>Electron cryo-microscopy (cryo-EM) has emerged over the past decade as the dominant technique in structural biology for determining the atomic structures of biological macromolecular complexes. One less appreciated feature of cryo-EM is that, in many cases, complexes do not need to be purified. Thus, the atomic structures of assemblies that can be found in biological samples as \u201ccontaminants\u201d can be readily determined. In this paper, the structures of filaments formed by a phosphatase and a protease are described.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02439-26","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T13:00:20Z","timestamp":1789390820000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Protein polymers proliferate: polymerization of phosphatases and proteases"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6212-2869","authenticated-orcid":false,"given":"Ravi R.","family":"Sonani","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0153tk833","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry and Molecular Genetics, University of Virginia Medical School","place":["Charlottesville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iman","family":"Chouikha","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035xkbk20","id-type":"ROR","asserted-by":"publisher"}],"name":"CNRS, Aix Marseille Universit\u00e9, LCB-UMR7283, IM2B","place":["Marseille, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9251-5639","authenticated-orcid":true,"given":"Thijs","family":"Bodson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/006e5kg04","id-type":"ROR","asserted-by":"publisher"}],"name":"Structural Biology Brussels, Department for Bio-engineering Sciences, Vrije Universiteit Brussel","place":["Brussels, Belgium"]},{"id":[{"id":"https:\/\/ror.org\/006e5kg04","id-type":"ROR","asserted-by":"publisher"}],"name":"Structural and Molecular Microbiology, Vlaams Instituut voor Biotechnology (VIB) Center for Structural Biology, Vrije Universiteit Brussels","place":["Brussels, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1878-0361","authenticated-orcid":true,"given":"Silvia","family":"Hidalgo-Martinez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/008x57b05","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biology, University of Antwerp","place":["Antwerp, Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5334-7655","authenticated-orcid":true,"given":"Filip J. 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