{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T01:58:10Z","timestamp":1781661490463,"version":"3.54.5"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T00:00:00Z","timestamp":1741046400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T00:00:00Z","timestamp":1741046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["21K07031"],"award-info":[{"award-number":["21K07031"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["24K10204"],"award-info":[{"award-number":["24K10204"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"MEXT | Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["22K15675"],"award-info":[{"award-number":["22K15675"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,3,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Elizabethkingia<\/jats:italic>\n              species, isolated from clinical and environmental samples, are emerging opportunistic bacterial pathogens with a high mortality rate in clinical settings worldwide. Taxonomically,\n              <jats:italic>Elizabethkingia<\/jats:italic>\n              comprises seven species:\n              <jats:italic>E. anophelis<\/jats:italic>\n              ,\n              <jats:italic>E. argenteiflava<\/jats:italic>\n              ,\n              <jats:italic>E. bruuniana<\/jats:italic>\n              ,\n              <jats:italic>E. meningoseptica<\/jats:italic>\n              ,\n              <jats:italic>E. miricola<\/jats:italic>\n              ,\n              <jats:italic>E. ursingii<\/jats:italic>\n              , and\n              <jats:italic>E. occulta<\/jats:italic>\n              . In this study, we identified useful biomarker proteins, including ribosomal L29, L30, S21, and the YtxH domain-containing proteins, for distinguishing\n              <jats:italic>Elizabethkingia<\/jats:italic>\n              species using matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiles. Evaluation of 29 clinical and environmental\n              <jats:italic>Elizabethkingia<\/jats:italic>\n              strains revealed that these species could be separated by MALDI-TOF MS profiles into six groups\n              <jats:italic>\u2014E. anophelis<\/jats:italic>\n              ,\n              <jats:italic>E. argenteiflava<\/jats:italic>\n              ,\n              <jats:italic>E. bruuniana\/E. miricola<\/jats:italic>\n              ,\n              <jats:italic>E. meningoseptica<\/jats:italic>\n              ,\n              <jats:italic>E. ursingii<\/jats:italic>\n              , and\n              <jats:italic>E. occulta<\/jats:italic>\n              \u2014based on the four biomarker protein peaks. This study demonstrates the potential of routine MALDI-TOF MS -based examination methods for the early detection of\n              <jats:italic>Elizabethkingia<\/jats:italic>\n              species in clinical laboratories.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              <jats:italic>Elizabethkingia<\/jats:italic>\n              species are groups of emerging opportunistic bacterial pathogens with a high mortality rate, causing healthcare-associated outbreaks worldwide. Rapid identification of\n              <jats:italic>Elizabethkingia<\/jats:italic>\n              species is important becausethese species show intrinsically carbapenem resistance and there are few data for using appropriate antibiotics. Until now, only whole-genome sequencing could accurately identify the seven\n              <jats:italic>Elizabethkingia<\/jats:italic>\n              species. Therefore, establishing rapid and accurate identification methods for\n              <jats:italic>Elizabethkingia<\/jats:italic>\n              species in clinical laboratories is vital. In this study, we developed new methods for identifying\n              <jats:italic>Elizabethkingia<\/jats:italic>\n              species using four biomarker protein peaks-ribosomal L29, L30, S21, and the YtxH domain-containing proteins by matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF MS) proteotyping. This study demonstrates the potential of routine MALDI-TOF MS -based laboratory examination for the early identification of\n              <jats:italic>Elizabethkingia<\/jats:italic>\n              species.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.02454-24","type":"journal-article","created":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T14:02:14Z","timestamp":1738850534000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Identification of\n            <i>Elizabethkingia<\/i>\n            species by MALDI-TOF MS proteotyping"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5519-7408","authenticated-orcid":false,"given":"Satomi","family":"Takei","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine","place":["Tokyo, Japan"]},{"name":"Department of MALDI-TOF MS Practical Application Research, Juntendo University Graduate School of Medicine","place":["Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanae","family":"Teramoto","sequence":"additional","affiliation":[{"name":"Department of MALDI-TOF MS Practical Application Research, Juntendo University Graduate School of Medicine","place":["Tokyo, Japan"]},{"name":"Analytical and Measurement Instruments Division, Shimadzu Corporation","place":["Kyoto, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1264-0617","authenticated-orcid":true,"given":"Yuji","family":"Sekiguchi","sequence":"additional","affiliation":[{"name":"Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)","place":["Tsukuba, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Miida","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine","place":["Tokyo, Japan"]},{"name":"Department of Clinical Medical Technology, Faculty of Medical Science, Juntendo University","place":["Urayasu, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7114-769X","authenticated-orcid":true,"given":"Teruo","family":"Kirikae","sequence":"additional","affiliation":[{"name":"Department of Microbiome Research, Juntendo University Graduate School of Medicine","place":["Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3053-1879","authenticated-orcid":true,"given":"Tatsuya","family":"Tada","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Juntendo University Graduate School of Medicine","place":["Tokyo, 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