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To remove this barrier, clinical studies have recently begun to implement the use of at-home capillary blood self-sampling devices in place of in-clinic venous blood draws for participant blood sample collection. Thus, we assessed antibody responses to a broad library of over 300 viral, bacterial, and fungal pathogens using VirScan immunoassay testing on adult volunteer capillary (serum) and venous (plasma) blood samples collected by Tasso devices and in-clinic venipunctures on the same day to determine whether self-sampling devices are a more practical and convenient alternative for future clinical studies. Most VirScan measurements of antibodies specific for clinically relevant respiratory viruses and herpesviruses had strong concordance between participant-matched Tasso and venipuncture blood samples. While we did not identify a systematic bias in most pathogen-specific antibody measurements associated with blood collection method, we did observe intrinsic VirScan inter-assay variability across some clinically relevant viruses. Together, our data demonstrate that capillary blood self-sampling devices are a practical alternative to in-clinic venipunctures for VirScan clinical research studies. However, these blood collection methods should not be used interchangeably within longitudinal studies to minimize the introduction of technical variables.<\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>Our study assessed whether capillary blood self-sampling devices could reliably replace in-clinic venous blood collection methods for the VirScan immunoassay, which can detect antibodies specific to hundreds of pathogens. Longitudinal studies requiring multiple in-clinic visits for sample collection often experience low volunteer retention because of the inconvenience of traveling to research sites. Allowing volunteers to use at-home self-sampling devices reduces the burden of travel for participants and increases access to outreach for volunteers who would otherwise not participate in research. Importantly, VirScan only requires a small sample volume, so blood self-sampling devices would be appropriate to use despite their volume collection limitations. Overall, capillary blood self-sampling devices can be a reliable and efficient method for research studies to investigate antibody responses longitudinally using VirScan. However, to limit the introduction of technical variables, collection methods should not be used interchangeably within a longitudinal study.<\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02454-25","type":"journal-article","created":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T09:06:03Z","timestamp":1777280763000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Assessing VirScan serosurvey epitope profiling variability between in-clinic venous blood draw and capillary blood self-sampling device"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3335-9258","authenticated-orcid":true,"given":"Linda M.","family":"Sircy","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/007ps6h72","id-type":"ROR","asserted-by":"publisher"}],"name":"Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center","place":["Seattle, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7546-8439","authenticated-orcid":true,"given":"Terry L.","family":"Stevens-Ayers","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/007ps6h72","id-type":"ROR","asserted-by":"publisher"}],"name":"Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center","place":["Seattle, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth M.","family":"Krantz","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/007ps6h72","id-type":"ROR","asserted-by":"publisher"}],"name":"Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center","place":["Seattle, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurel","family":"Joncas-Schronce","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/007ps6h72","id-type":"ROR","asserted-by":"publisher"}],"name":"Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center","place":["Seattle, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina S. 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