{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:34:32Z","timestamp":1787016872246,"version":"build-2736575974"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"MOST | National Key Research and Development Program of China","award":["2023YFC2308300"],"award-info":[{"award-number":["2023YFC2308300"]}]},{"name":"Theme-based Research Scheme","award":["T11-706\/18-N,T11-709\/24-N"],"award-info":[{"award-number":["T11-706\/18-N,T11-709\/24-N"]}]},{"name":"Shenzhen Clinical Research Center for Tuberculosis","award":["20210617141509001"],"award-info":[{"award-number":["20210617141509001"]}]},{"DOI":"10.13039\/501100019341","name":"Shenzhen Scientific and Technological Foundation","doi-asserted-by":"publisher","award":["nos. JSGG20220606141001003"],"award-info":[{"award-number":["nos. JSGG20220606141001003"]}],"id":[{"id":"10.13039\/501100019341","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shenzhen Clinical Medical Center for Emerging Infectious Diseases","award":["LCYSSQ20220823091203007"],"award-info":[{"award-number":["LCYSSQ20220823091203007"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Early bactericidal activity is a vital measure in developing new tuberculosis (TB) drugs. Traditional methods, including colony-forming units (CFUs) and time to positivity (TTP), have limitations. This study aimed to evaluate the efficacy of TPAPy-Tre fluorescence microscopy in monitoring early therapeutic responses compared with conventional culture methods in patients with pulmonary multidrug-resistant\/rifampicin-resistant TB. In an open-label clinical trial at the Third People\u2019s Hospital of Shenzhen, China, seven sputum smear-positive patients aged \u226518 years were enrolled. Sputum samples were consecutively analyzed using solid and liquid cultures and TPAPy-Tre microscopy. The study found that TPAPy-Tre fluorescence intensity significantly decreased from 271.5 (95% confidence interval [CI], 177.4\u2013365.7) before treatment to 142.8 (95% CI, 104.7\u2013180.9) after treatment (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.05). TPAPy-Tre results strongly correlated with CFU (Spearman \u03c1 = 0.60; 95% CI, 0.35\u20130.77;\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.001) and TTP (Spearman \u03c1 = \u22120.33; 95% CI, \u22120.56 to \u22120.04;\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.05). Among selected participants, the median fluorescence intensity decreased from 51.5 (interquartile range [IQR], 39.0\u201363.6) to 13.2 (IQR, 7.8\u201320.0) after treatment (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.001). TPAPy-Tre shows potential as a rapid, visual method for tracking bacterial vitality during TB treatment, offering immediate feedback on treatment response. These results support its use alongside conventional methods in clinical settings, though larger studies are needed for further validation.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>Early bactericidal activity (EBA) is an important tool in clinical studies in the development of new tuberculosis drugs. Current traditional methods of efficacy monitoring present significant limitations. There is a need for novel and efficient tools to monitor treatment response in real-time when EBA is performing.<\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02457-24","type":"journal-article","created":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T09:00:43Z","timestamp":1747904443000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["A cytoderm metabolic labeling TPAPy-Tre for real-time detection of vitality of\n                    <i>Mycobacterium tuberculosis<\/i>\n                    in sputum"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4249-4569","authenticated-orcid":false,"given":"Mengru","family":"Yang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01vy4gh70","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Public Health, Shenzhen University Medical School, Shenzhen University","place":["Shenzhen, China"]},{"name":"Shenzhen Clinical Research Center for Tuberculosis","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiqin","family":"Dai","sequence":"additional","affiliation":[{"name":"Institute of Hepatology, National Clinical Research Center for Infectious Diseases, Third People\u2019s Hospital of Shenzhen","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04xfsbk97","id-type":"ROR","asserted-by":"publisher"}],"name":"National Clinical Research Center for Infectious Diseases, Shenzhen Clinical Research Center for Tuberculosis, Shenzhen Third People\u2019s Hospital","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Hepatology, National Clinical Research Center for Infectious Diseases, Third People\u2019s Hospital of Shenzhen","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Senlin","family":"Zhan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04xfsbk97","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pulmonary Medicine and Tuberculosis, Third People\u2019s Hospital of Shenzhen","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjuan","family":"Qin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04xfsbk97","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pulmonary Medicine and Tuberculosis, Third People\u2019s Hospital of Shenzhen","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3590-4790","authenticated-orcid":false,"given":"Hongzhou","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Third People\u2019s Hospital of Shenzhen","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0463-7968","authenticated-orcid":false,"given":"Mingbin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Shenzhen Clinical Research Center for Tuberculosis","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4023-0228","authenticated-orcid":true,"given":"Peize","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01vy4gh70","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Public Health, Shenzhen University Medical School, Shenzhen University","place":["Shenzhen, China"]},{"name":"Shenzhen Clinical Research Center for Tuberculosis","place":["Shenzhen, China"]},{"id":[{"id":"https:\/\/ror.org\/04xfsbk97","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pulmonary Medicine and Tuberculosis, Third People\u2019s Hospital of Shenzhen","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","unstructured":"Organization WH. 2023. 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