{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T01:15:19Z","timestamp":1788225319079,"version":"build-2803163510"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["E-26\/210.071\/2024"],"award-info":[{"award-number":["E-26\/210.071\/2024"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,2,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Universal screening of pregnant individuals followed by intrapartum antibiotic prophylaxis is the most effective approach to prevent group B\n                      <jats:italic toggle=\"yes\">Streptococcus<\/jats:italic>\n                      (GBS) neonatal infections. Traditional protocols for GBS detection in rectovaginal samples involve a pre-enrichment step using selective broth, followed by inoculation onto blood agar, but these methods can be labor-intensive, time-consuming, and costly in low-resource settings. Here, we compared the performance of conventional selective broth prior to plating (THB-NAG) with direct plating onto chromogenic media (CA) for GBS detection among 1,128 pregnant individuals in Brazil. Overall, GBS was detected in 7.8% (88) of samples. CA detected a significantly (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.0001) higher number of positive samples (6.5%; 73) when compared to THB-NAG (2.7%; 31). CA was also superior in inhibiting contaminant microbiota, was faster and more cost-effective, and showed high sensitivity and accuracy levels when compared to THB-NAG, although it had failed when samples had low GBS density. Adopting more feasible and cost-effective protocols, such as the CA method shown in this study, can lead to improved adhesion to the universal antenatal GBS screening and thus, to the prevention of early GBS disease in low-resource settings, such as Brazil.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Group B\n                        <jats:italic toggle=\"yes\">Streptococcus<\/jats:italic>\n                        (GBS) is a leading cause of life-threatening neonatal infections. The recommended strategy to prevent GBS disease is based on GBS screening of pregnant individuals, followed by antibiotic prophylaxis. However, the standard laboratory method for GBS detection requires extended incubation time and may present challenges associated with costs and feasibility, making it difficult for routine implementation in low-resource settings. Additionally, the overgrowth of accompanying microbiota can hinder GBS detection, potentially reducing the method\u2019s sensitivity. This creates barriers to effective prevention strategies. In this context, chromogenic media have emerged as a promising alternative, offering faster, simpler, and more accurate detection through color-based differentiation of colonies. However, this method has not been fully evaluated in local healthcare conditions in countries like Brazil. This study was undertaken to investigate whether chromogenic media could provide a more practical, affordable, and reliable option for GBS detection in routine prenatal care.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02459-25","type":"journal-article","created":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T14:00:15Z","timestamp":1766498415000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Improved detection of group B\n                    <i>Streptococcus<\/i>\n                    in rectovaginal samples of pregnant individuals in Brazil using chromogenic medium"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9194-6445","authenticated-orcid":true,"given":"Natalia Silva","family":"Costa","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]},{"name":"Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ci\u00eancias M\u00e9dicas, Universidade do Estado do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1718-438X","authenticated-orcid":true,"given":"Andre","family":"Rio-Tinto","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia de Deus","family":"Santana","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Ferrarezi Favorato Moriel","family":"Garcia","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabella Bittencourt Ferreira","family":"Pinto","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laylla Graca","family":"Barros","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Clarisse","family":"Merces","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo Oliveira","family":"Bressan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura Maria Andrade","family":"Oliveira","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]},{"name":"Laborat\u00f3rio de Enterobact\u00e9rias, Instituto Oswaldo Cruz, FIOCRUZ","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio Eduardo Longo","family":"Fracalanzza","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4017-7076","authenticated-orcid":true,"given":"Lucia Martins","family":"Teixeira","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pen\u00e9lope Saldanha","family":"Marinho","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Maternidade Escola, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana Castro 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Group B Streptococcus vaccine: full value vaccine assessment. Available from: https:\/\/www.who.int\/publications-detail-redirect\/9789240037526. Retrieved 6 Mar 2025."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix664"},{"key":"e_1_3_2_4_2","unstructured":"American College of Obstetricians and Gynecologists. 2020. Prevention of group B streptococcal early-onset disease in newborns. ACOG. Retrieved 26 Feb 2025."},{"key":"e_1_3_2_5_2","unstructured":"World Health Organization. 2024. WHO recommendation on screening of pregnant women for intrapartum antibiotic prophylaxis for the prevention of early onset group B Streptococcus disease in newborns. Retrieved 6 Mar 2025."},{"key":"e_1_3_2_6_2","unstructured":"American Society for Microbiology. 2021. Guidelines for the detection and identification of group B Streptococcus. Available from: https:\/\/asm.org:443\/Guideline\/Guidelines-for-the-Detection-and-Identification-of. Retrieved 6 Mar 2025."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00097-16"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0196925"},{"key":"e_1_3_2_9_2","unstructured":"MedCalc. 1993. MedCalc\u2019s diagnostic test evaluation calculator. Available from: https:\/\/www.medcalc.org\/calc\/diagnostic_test.php. 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