{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:51:31Z","timestamp":1789692691720,"version":"4.0.1"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:00:00Z","timestamp":1777939200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:00:00Z","timestamp":1777939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,5,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      WHO released the first and second editions of\n                      <jats:italic toggle=\"yes\">Mycobacterium tuberculosis<\/jats:italic>\n                      complex (MTBC) mutations catalog associated with drug resistance in 2021 and 2023, respectively. The aim of this study was to evaluate the resistance prediction by mutation catalog for nine anti-tuberculosis drugs in rifampicin (RIF)-resistant tuberculosis (RR-TB) in Wenzhou, China. Over the period from 2020 to 2022, 301 RR-TB cases were continuously prospectively collected in Wenzhou Central Hospital. A total of 214 MTBC isolates were collected with drug susceptibility testing using the MGIT960 system and MYCOTB plates. Whole-genome sequencing (WGS) was performed for resistance prediction using mutation catalog by the WHO and freely available tools (TB-Profiler). Among 214 RR-TB isolates, drug resistance prediction using the WHO second edition mutation catalog demonstrated high sensitivity when stratified by the two predominant lineages. In Beijing lineage (L2,\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 171) isolates, sensitivity was 98.73% for RIF, 95.77% for isoniazid (INH), 89.29% for ethambutol (EMB), and 98.04% for moxifloxacin (MFX). Corresponding values in Euro-American lineage (L4,\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 43) isolates were 100.00% for RIF, 92.11% for INH, 94.44% for EMB, and 75.00% for MFX. Specificity exceeded 80% for all drugs except RIF in both lineages. Additionally, three potential resistance-associated mutations were determined (\n                      <jats:italic toggle=\"yes\">ahpC<\/jats:italic>\n                      _\u221252C&gt;T for INH,\n                      <jats:italic toggle=\"yes\">embA<\/jats:italic>\n                      _\u221211C&gt;A for EMB,\n                      <jats:italic toggle=\"yes\">eis<\/jats:italic>\n                      _\u221210G&gt;A for Kanamycin). Resistance prediction based on mutation catalog offers better patient-tailored treatment of tuberculosis. Integrating more new drug mutations from different geographic areas would improve the accuracy of resistance prediction.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Based on the catalog of mutations in\n                        <jats:italic toggle=\"yes\">Mycobacterium tuberculosis<\/jats:italic>\n                        complex (MTBC) and their association with drug resistance published by the WHO in 2021 and 2023, this study evaluated the predictive performance of the catalogs for resistance to nine anti-tuberculosis drugs among 214 MTBC isolates from Wenzhou Central Hospital. The results demonstrated high accuracy of resistance prediction using the second edition. Three potential resistance-associated mutations were also identified. These findings not only confirm the reliability of the current catalog but may also provide valuable insights for future updates.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02481-25","type":"journal-article","created":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T16:07:21Z","timestamp":1774973241000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["The performance of the WHO mutation catalog of\n                    <i>Mycobacterium tuberculosis<\/i>\n                    in the diagnosis of drug resistance to rifampicin-resistant strains in Wenzhou, China"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6700-6920","authenticated-orcid":false,"given":"Guiqing","family":"He","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00w5h0n54","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Infectious Diseases, Wenzhou Central Hospital, Affiliated to Wenzhou Medical University","place":["Wenzhou, China"]},{"id":[{"id":"https:\/\/ror.org\/00w5h0n54","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratory of Infectious Diseases, Wenzhou Central Hospital, Affiliated to Wenzhou Medical University","place":["Wenzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyong","family":"Zheng","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00w5h0n54","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratory of Infectious Diseases, Wenzhou Central Hospital, Affiliated to Wenzhou Medical University","place":["Wenzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013xs5b60","id-type":"ROR","asserted-by":"publisher"}],"name":"National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory on Drug-Resistant Tuberculosis, Beijing Chest Hospital, Capital Medical University","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjie","family":"Luan","sequence":"additional","affiliation":[{"name":"School of Public Health (Shenzhen), Shenzhen Campus of Sun Yat-sen University","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanfei","family":"Ji","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013xs5b60","id-type":"ROR","asserted-by":"publisher"}],"name":"National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory on Drug-Resistant Tuberculosis, Beijing Chest Hospital, Capital Medical University","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenzhen","family":"Zhou","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00w5h0n54","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Infectious Diseases, Wenzhou Central Hospital, Affiliated to Wenzhou Medical University","place":["Wenzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1400-7321","authenticated-orcid":true,"given":"Xia","family":"Yu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013xs5b60","id-type":"ROR","asserted-by":"publisher"}],"name":"National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory on Drug-Resistant Tuberculosis, Beijing Chest Hospital, Capital Medical University","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","unstructured":"World Health Organization. 2024. Global tuberculosis report 2024. Geneva World Health Organization"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201909-1819LE"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01550-17"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2024.2392656"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-019-0214-5"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD009593.pub3"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep17850"},{"key":"e_1_3_4_9_2","unstructured":"World Health Organization. 2021. Catalogue of mutations in Mycobacterium tuberculosis complex and their association with drug resistance. World Health Organization Geneva"},{"key":"e_1_3_4_10_2","volume-title":"Catalogue of mutations in Mycobacterium tuberculosis complex and their association with drug resistance","author":"World Health Organization","year":"2023","unstructured":"World Health Organization. 2023. Catalogue of mutations in Mycobacterium tuberculosis complex and their association with drug resistance. 2nd ed. World Health Organization, Geneva.","edition":"2"},{"key":"e_1_3_4_11_2","unstructured":"World Health Organization. 2018. Technical report on critical concentrations for drug susceptibility testing of medicines used in the treatment of drug-resistant tuberculosis. World Health Organization Geneva"},{"key":"e_1_3_4_12_2","unstructured":"World Health Organization. 2021. Technical report on critical concentrations for drug susceptibility testing of isoniazid and the rifamycins (rifampicin rifabutin and rifapentine). World Health Organization Geneva"},{"key":"e_1_3_4_13_2","volume-title":"CLSI supplement M24S","author":"Clinical and Laboratory Standards Institute","year":"2023","unstructured":"Clinical and Laboratory Standards Institute. 2023. Performance standards for susceptibility testing of Mycobacteria, Nocardia spp., and other aerobic actinomycetes. In CLSI supplement M24S, 2nd edition. 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