{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:04:08Z","timestamp":1785409448991,"version":"3.56.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82503692"],"award-info":[{"award-number":["82503692"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Scientific and Technological Projects in Henan Province","award":["242102311220"],"award-info":[{"award-number":["242102311220"]}]},{"name":"Key Scientific and Technological Projects in Henan Province","award":["242102310268"],"award-info":[{"award-number":["242102310268"]}]},{"name":"Henan Provincial Department of Science and Technology Key Technology Development Project","award":["252102311181"],"award-info":[{"award-number":["252102311181"]}]},{"name":"Henan Provincial Health and Health Commission Joint Construction Project","award":["LHGJ20230356"],"award-info":[{"award-number":["LHGJ20230356"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,6,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Meningiomas are prevalent intracranial tumors with poorly understood extraneural drivers. While the gut-brain axis influences neuro-oncogenesis, meningioma-specific gut microbiome alterations and their clinical implications remain uncharacterized. This study integrated 16S rRNA sequencing of fecal samples from 15 treatment-na\u00efve WHO grade I meningioma patients (MPs) and 15 healthy controls (HCs) with immunohistochemical profiling of tumor immune infiltrates (MPO\u207a neutrophils, CD68\u207a macrophages, CD3\u207a T cells). Compared with HCs, MPs exhibited significantly reduced alpha diversity (Shannon index,\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.026) and distinct beta diversity (permutational multivariate analysis of variance,\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.0009). Taxonomic analysis revealed enrichment of\n                      <jats:italic toggle=\"yes\">Proteobacteria<\/jats:italic>\n                      (28.82% vs. 2.46%,\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.001), specifically\n                      <jats:italic toggle=\"yes\">Escherichia_Shigella<\/jats:italic>\n                      at the genus level (25.95% vs. 1.61%,\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.008), along with depletion of\n                      <jats:italic toggle=\"yes\">Bacteroidaceae<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Ruminococcaceae<\/jats:italic>\n                      . LEfSe identified\n                      <jats:italic toggle=\"yes\">Escherichia_Shigella<\/jats:italic>\n                      as the top meningioma-enriched biomarker. In diagnostic modeling,\n                      <jats:italic toggle=\"yes\">Escherichia_Shigella<\/jats:italic>\n                      achieved an area under the receiver operating characteristic curve of 95.11% (95% CI: 86.91%\u2013100%) for WHO grade I meningioma detection. Critically,\n                      <jats:italic toggle=\"yes\">Escherichia_Shigella<\/jats:italic>\n                      abundance positively correlated with intratumoral MPO\u207a, CD68\u207a, and CD3\u207a cell densities (all\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.05), whereas\n                      <jats:italic toggle=\"yes\">Ruminococcaceae<\/jats:italic>\n                      showed inverse correlations. The 16S rRNA sequencing data are publicly available in the GSA database under accession number\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/ngdc.cncb.ac.cn\/gsa\/browse\/CRA027974\" xlink:type=\"simple\">CRA027974<\/jats:ext-link>\n                      . This study provides the first evidence of gut dysbiosis in grade I meningioma, characterized by\n                      <jats:italic toggle=\"yes\">Escherichia_Shigella<\/jats:italic>\n                      dominance and depletion of immunomodulatory commensals. This signature correlates with increased immune infiltration and holds promise as a novel biomarker.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Detailed exploration of host\u2013microbe interactions can be worthwhile. Gut dysbiosis has been implicated in neuroinflammation, blood\u2013brain barrier disruption, and oncogenesis in multiple cancer types, including gliomas. However, the gut microbiota composition and metabolic characteristics in patients with meningioma have not been previously reported. To address these critical knowledge gaps, we conducted a case\u2013control study integrating 16S rRNA sequencing, clinical phenotyping, and immunohistochemical profiling. Our study revealed significant alterations in the gut microbiota of MPs, characterized by reduced alpha diversity, enrichment of\n                      <jats:italic toggle=\"yes\">Proteobacteria<\/jats:italic>\n                      , and depletion of beneficial taxa, including\n                      <jats:italic toggle=\"yes\">Bacteroidaceae<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Ruminococcaceae<\/jats:italic>\n                      . Critically, we identified\n                      <jats:italic toggle=\"yes\">Escherichia_Shigella<\/jats:italic>\n                      as a potential diagnostic biomarker and demonstrated strong correlations between elevated\n                      <jats:italic toggle=\"yes\">Escherichia_Shigella<\/jats:italic>\n                      \/Enterobacteriaceae abundance and increased intratumoral infiltration of MPO\u207a neutrophils, CD68\u207a macrophages, and CD3\u207a T cells. These findings are the first evidence that gut microbiome dysbiosis is closely associated with meningioma inflammation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02485-25","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T13:04:12Z","timestamp":1777554252000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Gut microbiota composition and tumor immune features in meningioma patients"],"prefix":"10.1128","volume":"14","author":[{"given":"Kai","family":"Yin","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/056swr059","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Neurosurgery, The First Affiliated Hospital of Zhengzhou University","place":["Zhengzhou, 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