{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:51:15Z","timestamp":1786117875887,"version":"build-2736575974"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n The high rates of multi-drug resistance and increasing transmission rates in healthcare environments reported for\n Candida auris (C. auris<\/jats:italic>\n ) led to its categorization as an urgent threat in the latest Centers for Disease Control and Prevention (CDC)\u2019s Antibiotic Resistance Threats Report released in 2019.\n C. auris<\/jats:italic>\n skin colonization and shedding contribute to its high transmissibility and allow for its recovery from wastewater. The incorporation of\n C. auris<\/jats:italic>\n into wastewater surveillance workflows could aid in early detection of healthcare facilities with\n C. auris<\/jats:italic>\n emergence and could lead to better understanding of pathogen prevalence and trends in a healthcare system. The goal of this study was to validate a wastewater testing workflow for the detection of\n C. auris<\/jats:italic>\n using a quantitative polymerase chain reaction (qPCR) protocol originally developed for\n C. auris<\/jats:italic>\n colonization screening. Using a wastewater matrix, the assay showed 96.8% PCR amplification efficiency with a slope of \u22123.4 and high sensitivity with a limit of detection of 1.8 colony-forming units\/milliliters. The assay also showed high reproducibility. These results suggest that the qPCR assay used for the detection of\n C. auris<\/jats:italic>\n from clinical specimens can also be adapted for accurate detection of this pathogen from wastewater samples.\n <\/jats:p>\n <\/jats:sec>\n \n IMPORTANCE<\/jats:title>\n \n Candida auris<\/jats:italic>\n is an emerging, often multidrug-resistant, pathogen with the ability to cause severe infections. Due to its persistence on surfaces and fomites, it spreads efficiently in healthcare settings. Controlling transmission can be challenging because it can colonize skin, from which it is shed, further contaminating the healthcare environment. Patient screening is both time and cost-consuming, and limited proactive screening by healthcare facilities has resulted in insufficient monitoring. Wastewater surveillance offers an opportunity to better understand the prevalence of this pathogen within healthcare environments. Here, we validated a\n C. auris<\/jats:italic>\n colonization qPCR assay for use in detecting\n C. auris<\/jats:italic>\n in wastewater samples, a more cost-effective way to screen an entire healthcare system. Our results show that this assay can reliably detect\n C. auris<\/jats:italic>\n in wastewater, supporting its integration into existing wastewater surveillance workflows and allowing detection in areas where direct patient screening is not yet available.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1128\/spectrum.02508-25","type":"journal-article","created":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T13:01:59Z","timestamp":1782133319000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Validation of molecular detection of\n Candida auris<\/i>\n from wastewater"],"prefix":"10.1128","volume":"14","author":[{"given":"Roxanna M.","family":"Rodr\u00edguez Stewart","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00qzjvm58","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratory Leadership Service, Centers for Disease Control and Prevention","place":["Atlanta, USA"]},{"id":[{"id":"https:\/\/ror.org\/00qzjvm58","id-type":"ROR","asserted-by":"publisher"}],"name":"Mycotic Diseases Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention","place":["Atlanta, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1086-8041","authenticated-orcid":true,"given":"Anastasia P.","family":"Litvintseva","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00qzjvm58","id-type":"ROR","asserted-by":"publisher"}],"name":"Mycotic Diseases Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention","place":["Atlanta, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Chavez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00qzjvm58","id-type":"ROR","asserted-by":"publisher"}],"name":"Mycotic Diseases Branch, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention","place":["Atlanta, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3099-7334","authenticated-orcid":true,"given":"D. 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