{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:15:56Z","timestamp":1785413756823,"version":"3.56.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,9,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Infectious spleen and kidney necrosis virus (ISKNV) is the causative agent of a fatal disease in many fish species, resulting in mass mortalities and significant economic losses. Since its introduction to Ghana in late 2018 and in the absence of effective vaccines, the crude practice of heat-shock treatment (HST) on deliberately exposed cultured tilapia fingerlings was widely adopted by farmers to control the disease in Ghana with some apparent success. This study investigated the interplay between the expression of heat-shock proteins (HSPs) and viral replication during ISKNV infection. An\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      experimental challenge study which involved deliberate infection with ISKNV and subsequent exposure of primary\n                      <jats:italic toggle=\"yes\">Oreochromis niloticus<\/jats:italic>\n                      (tilapia) brain cell lines to HST at 48 hours post-infection was carried out. The ISKNV was confirmed by Oxford Nanopore Sequencing of the full major capsid protein (MCP), while the species identity of the cell line was confirmed by Sanger sequencing of the cytochrome C oxidase (COX1) genomic region. The test groups and control groups were screened at various time points for viral proliferation and HSP marker expression using quantitative PCR (qPCR). Exposure to heat shock significantly increased HSP 90 and 47 expressions by fourfold and sixfold, respectively, with a concomitant 10-fold decrease in viral load as compared to the non-heat-shock group. Viral apoptosis gene\n                      <jats:italic toggle=\"yes\">ORF 005L<\/jats:italic>\n                      was significantly downregulated following increase in HSPs expression. This initial finding implies that HST may play an important role in suppressing viral replication through the apoptosis regulatory gene\n                      <jats:italic toggle=\"yes\">ORF 005L<\/jats:italic>\n                      . This information will contribute to the understanding of the beneficial effect of heat-shock therapy used in control of the viral pathogen in aquaculture. Further studies in controlled\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      experiments will give more clarity to the general effect of this treatment on tilapia growth and ISKNV persistence in infected fish populations.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>Infection of tilapia by infectious spleen and kidney necrosis virus (ISKNV) is trending toward endemicity in Ghanaian lake-based tilapia farms, and it is an important fish pathogen, worldwide. This study provides a potential mechanism to explain the reported role of heat-shock in protecting fish from the negative effects of ISKNV infection. Thus, it offers strong evidence for heat-shock therapy and will lead to better disease management in Ghana and worldwide. Additionally, there are other research avenues that may lead to some therapeutic options down the line.<\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02510-24","type":"journal-article","created":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T10:44:07Z","timestamp":1754995447000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Investigating the mechanism of heat-shock protection in ISKNV-infected tilapia brain cell line"],"prefix":"10.1128","volume":"13","author":[{"given":"Angela Naa Amerley","family":"Ayiku","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Cell and Molecular Biology, University of Ghana","place":["Accra, Ghana"]},{"name":"West African Centre for Cell Biology of Infectious Pathogens, University of Ghana","place":["Accra, Ghana"]},{"id":[{"id":"https:\/\/ror.org\/01r22mr83","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Basic and Applied Sciences, University of Ghana","place":["Accra, Ghana"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene Amoakoh","family":"Owusu","sequence":"additional","affiliation":[{"name":"West African Centre for Cell Biology of Infectious Pathogens, University of Ghana","place":["Accra, Ghana"]},{"id":[{"id":"https:\/\/ror.org\/01r22mr83","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Basic and Applied Sciences, University of Ghana","place":["Accra, Ghana"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Verner-Jeffreys","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04bd4pk40","id-type":"ROR","asserted-by":"publisher"}],"name":"WorldFish","place":["Bayan Lepas, Malaysia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Paley","sequence":"additional","affiliation":[{"name":"Cefas Weymouth Laboratory, Centre for Environment, Fisheries and Aquaculture Science","place":["Weymouth, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter Kojo","family":"Quashie","sequence":"additional","affiliation":[{"name":"West African Centre for Cell Biology of Infectious Pathogens, University of Ghana","place":["Accra, Ghana"]},{"id":[{"id":"https:\/\/ror.org\/01r22mr83","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Basic and Applied Sciences, University of Ghana","place":["Accra, Ghana"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2791-8949","authenticated-orcid":true,"given":"Samuel","family":"Duodu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Cell and Molecular Biology, University of Ghana","place":["Accra, Ghana"]},{"name":"West African Centre for Cell Biology of Infectious Pathogens, University of Ghana","place":["Accra, Ghana"]},{"id":[{"id":"https:\/\/ror.org\/01r22mr83","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Basic and Applied Sciences, University of Ghana","place":["Accra, Ghana"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"896","article-title":"First report of Megalocytivirus (Iridoviridae) in grouper culture in Sabah, Malaysia","volume":"3","author":"Razak AA","year":"2014","unstructured":"Razak AA, Ransangan J, Sade A. 2014. 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