{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T01:15:13Z","timestamp":1788225313976,"version":"build-2803163510"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,2,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Early detection of\n                      <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                      carbapenemase (KPC)-producing\n                      <jats:italic toggle=\"yes\">Enterobacterales<\/jats:italic>\n                      enables prompt initiation of appropriate antimicrobial therapy and infection prevention and control measures. The matrix-assisted laser desorption\/ionization time-of-flight (MALDI-ToF) Biotyper (MBT) Subtyping Module (Bruker Daltonics) uses a characteristic spectral peak at ~11,109 m\/z to identify KPC-producing\n                      <jats:italic toggle=\"yes\">Enterobacterales<\/jats:italic>\n                      . The aim of this study was to assess the ability of the MBT Module to detect KPC among a variety of clinical and reference (AR Bank)\n                      <jats:italic toggle=\"yes\">Enterobacterales<\/jats:italic>\n                      isolates. Detection of KPC was compared between the MBT Module and a laboratory-developed real-time KPC PCR. Of the 93 isolates tested, 58 (62.4%) were positive for KPC by PCR (39 clinical and 19 AR bank isolates), and 35 were negative. Among the KPC-positive PCR isolates, nine (seven clinical and two AR bank) also tested positive for KPC by the MBT Module. KPC detection via the MBT Module had a sensitivity of 15.5% and a specificity and positive predictive value of 100%. This study also assessed the performance of the MBT Module to detect KPC-2 versus KPC-3 among the AR Bank isolates. The MBT Module detected 2\/5 (40.0%) KPC-2 and 0\/14 of KPC-3 AR Bank isolates. As sensitivity was low, but specificity was high, a positive MBT Module result reliably predicts the presence of KPC; however, negative results cannot be relied upon to be truly KPC-negative.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Enterobacterales<\/jats:italic>\n                      , a large order of pathogenic and commensal bacteria, can gain resistance to carbapenems through the acquisition of plasmids carrying genes, such as\n                      <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                      carbapenemase (KPC). Early detection of KPC is crucial for patient care; however, current detection methods that involve multiple time-consuming steps, commonly only undertaken after overnight susceptibility testing, indicate possible carbapenem resistance. This study examined the ability of matrix-assisted laser desorption\/ionization time-of-flight (MALDI-ToF), a common diagnostic tool in the clinical laboratory, to detect KPC among\n                      <jats:italic toggle=\"yes\">Enterobacterales<\/jats:italic>\n                      . KPC detection via MALDI-ToF had low sensitivity but 100% specificity. These results support and expand the microbiological and geographical range of prior publications for this tool. A practical application of this highly specific method would involve rapid triaging of KPC-positive isolates a day earlier than current methods, permitting initiation of more timely therapeutic and infection prevention and control measures.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02511-25","type":"journal-article","created":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T12:34:16Z","timestamp":1766752456000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the Bruker MALDI Biotyper (MBT) subtyping module for detection of\n                    <i>Klebsiella pneumoniae<\/i>\n                    carbapenemase (KPC) in\n                    <i>Enterobacterales<\/i>\n                    in a Canadian clinical microbiology laboratory"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3287-041X","authenticated-orcid":true,"given":"Emma","family":"Finlayson-Trick","sequence":"first","affiliation":[{"name":"Division of Medical Microbiology & Infection Control, Vancouver Coastal Health Authority","place":["Vancouver, Canada"]},{"name":"Department of Pathology and Laboratory Medicine, University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Glassman","sequence":"additional","affiliation":[{"name":"Division of Medical Microbiology & Infection Control, Vancouver Coastal Health Authority","place":["Vancouver, Canada"]},{"name":"Department of Pathology and Laboratory Medicine, University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1714-5548","authenticated-orcid":true,"given":"Jasmine","family":"Ahmed-Bentley","sequence":"additional","affiliation":[{"name":"Division of Medical Microbiology & Infection Control, Vancouver Coastal Health Authority","place":["Vancouver, Canada"]},{"name":"Department of Pathology and Laboratory Medicine, University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhe","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Medical Microbiology & Infection Control, Vancouver Coastal Health Authority","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Tsui","sequence":"additional","affiliation":[{"name":"Division of Medical Microbiology & Infection Control, Vancouver Coastal Health Authority","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lori","family":"Sung","sequence":"additional","affiliation":[{"name":"Division of Medical Microbiology & Infection Control, Vancouver Coastal Health Authority","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlene","family":"Porter","sequence":"additional","affiliation":[{"name":"Division of Medical Microbiology & Infection Control, Vancouver Coastal Health Authority","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudine","family":"Desruisseaux","sequence":"additional","affiliation":[{"name":"Division of Medical Microbiology & Infection Control, Vancouver Coastal Health Authority","place":["Vancouver, Canada"]},{"name":"Department of Pathology and Laboratory Medicine, University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valery","family":"Lavergne","sequence":"additional","affiliation":[{"name":"Division of Medical Microbiology & Infection Control, Vancouver Coastal Health Authority","place":["Vancouver, Canada"]},{"name":"Department of Pathology and Laboratory Medicine, University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suefay","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Medical Microbiology & Infection Control, Vancouver Coastal Health Authority","place":["Vancouver, Canada"]},{"name":"Department of Pathology and Laboratory Medicine, University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Tat","sequence":"additional","affiliation":[{"name":"Division of Medical Microbiology & Infection Control, Vancouver Coastal Health Authority","place":["Vancouver, Canada"]},{"name":"Department of Pathology and Laboratory Medicine, University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Lieu","sequence":"additional","affiliation":[{"name":"Division of Medical Microbiology & Infection Control, Vancouver Coastal Health Authority","place":["Vancouver, Canada"]},{"name":"Department of Pathology and Laboratory Medicine, University of British Columbia","place":["Vancouver, Canada"]},{"id":[{"id":"https:\/\/ror.org\/03rmrcq20","id-type":"ROR","asserted-by":"publisher"}],"name":"Deparment of Medicine, Division of Infectious Diseases, University of British Columbia","place":["Vancouver, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aruna Uma","family":"Chandran","sequence":"additional","affiliation":[{"name":"Division of Medical Microbiology & Infection Control, Vancouver Coastal Health 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WHO Bacterial Priority Pathogens List, 2024: bacterial pathogens of public health importance to guide research, development and strategies to prevent and control antimicrobial resistance."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11061491"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.14745\/ccdr.v49i05a09"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed10040115"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofad500.2086"},{"key":"e_1_3_3_7_2","unstructured":"Antimicrobial Resistance. 2024. Canadian antimicrobial resistance surveillance system 2024. Available from: https:\/\/health-infobase.canada.ca\/carss\/amr\/results.html?ind=09#furtherReading. Retrieved 14 May 2025."},{"key":"e_1_3_3_8_2","unstructured":"Provincial Infection Control Network of British Columbia (PICNet). 2019. Annual surveillance report of healthcare-associated infections in BC health care facilities: fiscal year 2018\/2019."},{"key":"e_1_3_3_9_2","unstructured":"Provincial Infection Control Network of British Columbia (PICNet). 2025. Quarterly surveillance reports. https:\/\/picnet.ca\/surveillance\/surveillance-reports\/quarterly-reports."},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00694-14"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01643-15"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01242-16"},{"key":"e_1_3_3_13_2","unstructured":"MALDI Biotyper subtyping module. 2024. 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