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Although there have been sporadic discoveries of myxobacterial species unable to fruit, it is unclear whether the non-fruiting characteristic is due to taxon-specific genetic deficiency or suboptimal cultivation conditions.\n                      <jats:italic toggle=\"yes\">Aggregicoccus<\/jats:italic>\n                      is a non-fruiting myxobacterial genus typified by a single validly published species,\n                      <jats:italic toggle=\"yes\">Ag. edonensis<\/jats:italic>\n                      . In this study, we report five novel\n                      <jats:italic toggle=\"yes\">Aggregicoccus<\/jats:italic>\n                      strains, which are classified into three novel type species\n                      <jats:italic toggle=\"yes\">, Ag. lacus<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Ag. agri<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">Ag. guangxiensis<\/jats:italic>\n                      , based on polyphasic taxonomic analysis. All the\n                      <jats:italic toggle=\"yes\">Aggregicoccus<\/jats:italic>\n                      strains are unable to produce fruiting bodies, but can still sporulate. We compared the genome differences between\n                      <jats:italic toggle=\"yes\">Aggregicoccus<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Myxococcus<\/jats:italic>\n                      ; both genera belong to the Myxococcaceae family, and all the genomes are of similar sizes. The results showed that the\n                      <jats:italic toggle=\"yes\">Aggregicoccus<\/jats:italic>\n                      strains are inherently deficient in the fruiting body-associated genomic information (FAGI). We propose an assessment of FAGI for the classification of non-fruiting myxobacterial species.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Fruiting body formation is traditionally regarded as a defining trait of myxobacteria. Here, we report that\n                        <jats:italic toggle=\"yes\">Aggregicoccus<\/jats:italic>\n                        spp., including six strains of four species, can sporulate but are deficient in the fruiting body-associated genomic information (FAGI). This demonstrates that the non-fruiting characteristic in\n                        <jats:italic toggle=\"yes\">Aggregicoccus<\/jats:italic>\n                        stems from inherent genetic deficiencies rather than suboptimal cultivation. Our findings highlight the need to assess FAGI presence in classifying non-fruiting lineages, innovate the isolation method, and refine our understanding of the diversity and evolution of the myxobacteria.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02535-25","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T13:01:01Z","timestamp":1788440461000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["<i>Aggregicoccus<\/i>\n                    is a myxobacterial genus inherently deficient in fruiting genes"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-0572-4369","authenticated-orcid":false,"given":"Xinran","family":"Du","sequence":"first","affiliation":[{"name":"State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University","place":["Qingdao, People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5752-9050","authenticated-orcid":true,"given":"Jianing","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University","place":["Qingdao, People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04rdtx186","id-type":"ROR","asserted-by":"publisher"}],"name":"Haide College, Ocean University of China","place":["Qingdao, People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yihan","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University","place":["Qingdao, People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University","place":["Qingdao, People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2998-8617","authenticated-orcid":false,"given":"Zhiwei","family":"Su","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02c9qn167","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Agriculture, Guangxi University","place":["Nanning, People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8336-6638","authenticated-orcid":true,"given":"Yue-zhong","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University","place":["Qingdao, People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.00071-25"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-30747-8_3"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.50374"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.129.2.770-777.1977"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-2191-1_26"},{"key":"e_1_3_4_7_2","volume-title":"Bergey\u2019s manual of systematic bacteriology","author":"Brenner DJ","year":"2005","unstructured":"Brenner DJ, Krieg NR, Staley JT. 2005. 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