{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T17:03:03Z","timestamp":1788282183408,"version":"build-2803163510"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Profilin is a small actin-binding protein that plays an important role in actin polymerization. However, its functions in\n                      <jats:italic>Candida albicans<\/jats:italic>\n                      , the most prevalent fungal pathogen, remain unclear. Here, we report that profilin plays a crucial role in\n                      <jats:italic>C. albicans<\/jats:italic>\n                      morphogenesis and virulence. Deletion of profilin results in abnormal morphogenesis and impaired hyphal development. Furthermore,\n                      <jats:italic>pfy1\u0394\/\u0394<\/jats:italic>\n                      is hypersensitive to cell wall stress and displays thicker cell wall than wild-type cells, indicative of a critical function of Pfy1 in cell wall integrity. In addition, our findings demonstrate that profilin is required for the virulence of\n                      <jats:italic>C. albicans<\/jats:italic>\n                      in a murine model of systemic infection. In conclusion, our work provides a promising target for developing antifungal drugs.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Our research revealed Pfy1 is not only involved in hyphal development but also essential for pseudohyphal formation in response to DNA damage agents methyl methanesulfonate (MMS) and H\n                        <jats:sub>2<\/jats:sub>\n                        O\n                        <jats:sub>2.<\/jats:sub>\n                        The disruption of\n                        <jats:italic>PFY1<\/jats:italic>\n                        resulted in striking morphological defects in both yeast and hyphal forms. Further investigation suggested that profilin plays a role in polarized growth of\n                        <jats:italic>Candida albicans<\/jats:italic>\n                        via binding with Act1, and contributes to cell wall remodeling. Both hyphal growth and cell wall integrity are the important virulence factors of\n                        <jats:italic>C. albicans<\/jats:italic>\n                        . Thus,\n                        <jats:italic>pfy1\u0394\/\u0394<\/jats:italic>\n                        strains significantly reduced mortality rates in mice. These findings suggested that profilin could serve as a target for developing new antifungal drugs possibly for use in combination therapies with caspofungin, for treating invasive candidiasis.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02593-24","type":"journal-article","created":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T09:02:27Z","timestamp":1740387747000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Profilin Pfy1 is critical for cell wall integrity and virulence in\n                    <i>Candida albicans<\/i>"],"prefix":"10.1128","volume":"13","author":[{"given":"Xun","family":"Sun","sequence":"first","affiliation":[{"name":"School of Life Sciences, Beijing University of Chinese Medicine","place":["Beijing, China"]},{"name":"The Third Clinical Medical College of the Three Gorges University, Gezhouba Central Hospital of Sinopharm","place":["Yichang, China"]},{"name":"Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, School of Basic Medicine, China Three Gorges University","place":["Yichang, China"]},{"name":"Yichang Key Laboratory of Infection and Inflammation, School of Basic Medicine, China Three Gorges University","place":["Yichang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9507-6563","authenticated-orcid":true,"given":"Yueqing","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Beijing University of Chinese Medicine","place":["Beijing, China"]},{"name":"Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, School of Basic Medicine, China Three Gorges University","place":["Yichang, China"]},{"name":"Yichang Key Laboratory of Infection and Inflammation, School of Basic Medicine, China Three Gorges University","place":["Yichang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomin","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Beijing University of Chinese Medicine","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Xiang","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, School of Basic Medicine, China Three Gorges University","place":["Yichang, China"]},{"name":"Yichang Key Laboratory of Infection and Inflammation, School of Basic Medicine, China Three Gorges University","place":["Yichang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Zou","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, School of Basic Medicine, China Three Gorges University","place":["Yichang, China"]},{"name":"Yichang Key Laboratory of Infection and Inflammation, School of Basic Medicine, China Three Gorges University","place":["Yichang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowen","family":"Liu","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, School of Basic Medicine, China Three Gorges University","place":["Yichang, China"]},{"name":"Yichang Key Laboratory of Infection and Inflammation, School of Basic Medicine, China Three Gorges University","place":["Yichang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Luo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Microbiology and Parasitology of Education Department of Guizhou, School of Basic Medical Science, Guizhou Medical University","place":["Guizhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7944-9147","authenticated-orcid":true,"given":"Qi","family":"Han","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Beijing University of Chinese Medicine","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-022-01112-0"},{"key":"e_1_3_3_3_2","unstructured":"WHO. 2022. 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