{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:15:45Z","timestamp":1785417345856,"version":"3.56.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20GM113117"],"award-info":[{"award-number":["P20GM113117"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["E-26\/210.505\/2019, E-26\/210.209\/2018, E-26\/010.002155\/2019, SEI-260003\/001213\/2020, E-26\/211.554\/2019, E-26\/010.001280\/2016, E-26\/201.328\/2021"],"award-info":[{"award-number":["E-26\/210.505\/2019, E-26\/210.209\/2018, E-26\/010.002155\/2019, SEI-260003\/001213\/2020, E-26\/211.554\/2019, E-26\/010.001280\/2016, E-26\/201.328\/2021"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["Finance code- 001"],"award-info":[{"award-number":["Finance code- 001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,12,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Staphylococcus<\/jats:italic>\n                      spp. are generally considered non-motile due to the absence of active motility structures. However,\n                      <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Staphylococcus xylosus<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">Staphylococcus epidermidis<\/jats:italic>\n                      have demonstrated passive movement. This study investigated the motility of eight additional\n                      <jats:italic toggle=\"yes\">Staphylococcus<\/jats:italic>\n                      species in semisolid media. While most species exhibited some motility,\n                      <jats:italic toggle=\"yes\">Staphylococcus pseudintermedius<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Staphylococcus schleiferi<\/jats:italic>\n                      displayed significantly higher levels of motility. Then, we further evaluated the motility of 49\n                      <jats:italic toggle=\"yes\">S<\/jats:italic>\n                      .\n                      <jats:italic toggle=\"yes\">pseudintermedius<\/jats:italic>\n                      and 30\n                      <jats:italic toggle=\"yes\">S<\/jats:italic>\n                      .\n                      <jats:italic toggle=\"yes\">schleiferi<\/jats:italic>\n                      isolates, revealing strain-dependent variations. The influence of glucose, NaCl, temperature (35 and 39\u00b0C), and culture media (tryptic soy broth [TSB], brain heart infusion [BHI], and Luria Bertani [LB]) on the motility of these two species was evaluated. Glucose and NaCl induced morphological changes in the\n                      <jats:italic toggle=\"yes\">S. pseudintermedius<\/jats:italic>\n                      colonies. In\n                      <jats:italic toggle=\"yes\">S. schleiferi<\/jats:italic>\n                      , NaCl decreased, while glucose increased motility. Temperature and culture media also significantly affected motility, with\n                      <jats:italic toggle=\"yes\">S. schleiferi<\/jats:italic>\n                      showing reduced movement in BHI and both species exhibiting decreased motility in LB compared to TSB. Shedding light on surface motility in\n                      <jats:italic toggle=\"yes\">Staphylococcus<\/jats:italic>\n                      , an understudied phenomenon, can provide insights to elucidate their biology and clarify their mechanism of colonization, survival, and pathogenesis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      The ability to move is an important characteristic for many bacteria to colonize and infect hosts, yet motility in\n                      <jats:italic toggle=\"yes\">Staphylococcus<\/jats:italic>\n                      is understudied. Our work reveals that several species, notably\n                      <jats:italic toggle=\"yes\">S. pseudintermedius<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">S. schleiferi<\/jats:italic>\n                      , can actively move across surfaces. This previously hidden capability may be crucial for how these bacteria spread and cause infections. Our evaluation of how different growth conditions and specific genes impact this trait shows species-dependent effects. This study opens new research avenues for understanding the mechanisms these bacteria use to spread within both the environment and their hosts.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02620-25","type":"journal-article","created":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T14:00:37Z","timestamp":1762437637000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Revealing hidden moves:\n                    <i>Staphylococcus<\/i>\n                    spp. exhibit motility in semisolid media"],"prefix":"10.1128","volume":"13","author":[{"given":"Jessica F. S.","family":"Lopes","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Microbiologia Paulo de G\u00f3es, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiago F. S.","family":"Coimbra","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Microbiologia Paulo de G\u00f3es, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thais","family":"Glatthardt","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Microbiologia Paulo de G\u00f3es, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]},{"id":[{"id":"https:\/\/ror.org\/03yjb2x39","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Physiology and Pharmacology, Health Research Innovation Centre, University of Calgary","place":["Calgary, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara Maria Guimar\u00e3es","family":"Silva","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Microbiologia Paulo de G\u00f3es, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K\u00e1tia Regina Netto","family":"dos Santos","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Microbiologia Paulo de G\u00f3es, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7880-9270","authenticated-orcid":true,"given":"Bruno A.","family":"Penna","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02rjhbb08","id-type":"ROR","asserted-by":"publisher"}],"name":"Universidade Federal Fluminense","place":["Niter\u00f3i, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7162-0026","authenticated-orcid":true,"given":"Rosana B. R.","family":"Ferreira","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03490as77","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Microbiologia Paulo de G\u00f3es, Universidade Federal do Rio de Janeiro","place":["Rio de Janeiro, Brazil"]},{"id":[{"id":"https:\/\/ror.org\/001tmjg57","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biosciences, The University of Kansas","place":["Lawrence, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24021707"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-017-2507-z"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1900"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2008.02.003"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/br.36.4.478-503.1972"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01635-06"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.14.31"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2011.04.007"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00170"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2011.04.003"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044646"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.157"},{"key":"e_1_3_2_14_2","unstructured":"Euzeby JP. 2025. List of prokaryotic names with standing in nomenclature- genus Staphylococcus. Available from: https:\/\/www.bacterio.net"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00109-13"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.11188"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2016.05.008"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01308-06"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11030581"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-022-04511-7"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02539-19"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(00)04403-2"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01276-10"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03157-12"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13330"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofz032"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.04670-22"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1136\/bcr-2020-238302"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed4020088"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63413-0"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00221"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/vde.13040"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.01.039"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2012.01046.x"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2016.2048"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.14895"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.02.012"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-38-2-168"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.2460\/javma.239.12.1566"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.2020.21.e11"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.15012"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12444"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.02620-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.02620-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T14:06:13Z","timestamp":1764684373000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.02620-25"}},"subtitle":[],"editor":[{"given":"Ethel","family":"Bayer-Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,12,2]]},"references-count":41,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12,2]]}},"alternative-id":["10.1128\/spectrum.02620-25"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.02620-25","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,2]]},"assertion":[{"value":"2025-08-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02620-25"}}