{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:35:21Z","timestamp":1787016921422,"version":"build-2736575974"},"reference-count":61,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T00:00:00Z","timestamp":1772496000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T00:00:00Z","timestamp":1772496000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,3,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                      is a pathobiont whose primary human reservoirs are nares, pharynx, intestines, and skin. When specific conditions in the host are altered, it can cause a wide variety of human diseases, including bacteremia and sepsis. Preclinical\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      models mimicking the most severe\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      infections in humans have been used to develop treatments against this pathogen. This study aims to better characterize a murine model of\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      bacteremia and sepsis, offering a new and more comprehensive view of the complex interactions between\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      and the host while better reflecting human disease dynamics. We investigated the kinetics of bacteria in blood, kidneys, and liver after infection with four strains representative of epidemiologically relevant\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      clonal lineages. After intravenous infection, bacteria progress through three major pathogenesis phases: (i) colony-forming units counts in blood decrease rapidly within 1\u20132 h as bacteria are captured by the liver, the first line of defense against blood-borne bacteria; (ii) mice begin to show signs of acute disease, and bacteria disseminate to the kidneys where they grow quickly, reaching the peak in 1\u20132 days; (iii) bacteria establish an equilibrium with the host, forming abscesses in the kidneys while persisting in low numbers in the blood. These phases are common to all the tested\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      strains, although some strain-specific peculiarities have also been identified. Our findings could help improve understanding of host-pathogen interactions in\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      infections and their implications for human health, potentially laying the groundwork for developing novel preventive and therapeutic strategies.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Our work provides new insights into the interaction between\n                        <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                        and the host in a mouse model of bloodstream infection and sepsis. We found similarities between findings in the mouse model and human disease, underscoring the importance of using this laboratory host to study new therapeutic and preventive interventions. The comprehensive approach we used, utilizing several epidemiologically relevant\n                        <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                        clones and two distinct mouse strains, enhances the relevance of our results and sheds light on the complex interaction between this human pathogen and a widely used laboratory research host. We believe this approach could also be useful for studying\n                        <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                        infections in different animal models of disease.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02642-25","type":"journal-article","created":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T14:00:11Z","timestamp":1768917611000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Unraveling the mouse model of\n                    <i>Staphylococcus aureus<\/i>\n                    bacteremia and sepsis: a systematic approach to better characterize host\/pathogen interactions"],"prefix":"10.1128","volume":"14","author":[{"given":"Serena","family":"Vastola","sequence":"first","affiliation":[{"name":"GSK","place":["Siena, Italy"]},{"id":[{"id":"https:\/\/ror.org\/02p77k626","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biology, University of Rome Tor Vergata","place":["Rome, Italy"]},{"id":[{"id":"https:\/\/ror.org\/02p77k626","id-type":"ROR","asserted-by":"publisher"}],"name":"PhD Program in Evolutionary Biology and Ecology, Department of Biology, University of Rome \u201cTor Vergata\u201d","place":["Rome, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Tortoli","sequence":"additional","affiliation":[{"name":"GSK","place":["Siena, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Torricelli","sequence":"additional","affiliation":[{"name":"GSK","place":["Siena, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michela","family":"Brazzoli","sequence":"additional","affiliation":[{"name":"GSK","place":["Siena, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco Maria","family":"D'Andrea","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02p77k626","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biology, University of Rome Tor Vergata","place":["Rome, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5574-116X","authenticated-orcid":true,"given":"Emiliano","family":"Chiarot","sequence":"additional","affiliation":[{"name":"GSK","place":["Siena, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.1.1054.369"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02419"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/14787210.2017.1358611"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.00028-25:e0002825"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.001940"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2013.03.020"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(24)00040-5"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2012.03914.x"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(25)00118-5"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00081-09"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0147-4"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lanmic.2024.07.003"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1086\/533452"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/156.5.741"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/157.4.723"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1086\/432472"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciz257"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2014.04.007"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.09-135467"},{"key":"e_1_3_5_21_2","first-page":"203","article-title":"The establishment of staphylococcal abscesses in the mouse kidney","volume":"39","author":"Gorrill RH","year":"1958","unstructured":"Gorrill RH. 1958. 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