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Since the end of the last century,\n                      <jats:italic toggle=\"yes\">Aedes japonicus<\/jats:italic>\n                      has continued to spread in Europe as well as in North America.\n                      <jats:italic toggle=\"yes\">Aedes japonicus<\/jats:italic>\n                      is known to be able to transmit several viruses, but extensive information about the vector competence of\n                      <jats:italic toggle=\"yes\">Ae. japonicus<\/jats:italic>\n                      for alphaviruses is missing. Therefore, we infected field-caught\n                      <jats:italic toggle=\"yes\">Ae. japonicus<\/jats:italic>\n                      mosquitoes from Germany with different alphaviruses that occur in areas with either tropical or moderate temperatures and are clustered as arthritogenic or encephalitic alphaviruses. Additionally, we studied the influence of temperature and natural infections with insect-specific viruses (ISVs) on the vector competence of\n                      <jats:italic toggle=\"yes\">Ae. japonicus<\/jats:italic>\n                      . Transmission of chikungunya virus was exclusively observed at the high-temperature profile of 27\u00b0 \u00b1 5\u00b0C, with a low transmission rate of 2.9%. Transmission of Sindbis virus and western equine encephalitis virus was observed at all investigated temperature profiles with higher transmission rates of 32%\u201357%. We identified seven different ISVs in the investigated\n                      <jats:italic toggle=\"yes\">Ae. japonicus<\/jats:italic>\n                      mosquitoes, showing that coinfections with ISVs are very common. The interplay between arbovirus infections and concurrent multiple ISV infections is highly complex, and additional research is required to fully elucidate the detailed mechanisms underlying the outcomes of this study.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      The spread of invasive mosquito species like\n                      <jats:italic toggle=\"yes\">Aedes japonicus<\/jats:italic>\n                      poses a significant public health risk, particularly in the context of rising global temperatures and the growing prevalence of arbovirus infections. This study provides critical insights into the ability of\n                      <jats:italic toggle=\"yes\">Aedes japonicus<\/jats:italic>\n                      to transmit alphaviruses such as chikungunya, Sindbis, and western equine encephalitis under different temperature conditions. The identification of multiple insect-specific viruses co-infecting the mosquitoes highlights the complexity of arbovirus transmission and underscores the need for further research. Understanding the interplay between environmental factors like temperature and viral coinfections is essential for predicting and mitigating future outbreaks. This work advances our knowledge of vector competence, which is helpful for developing strategies for risk assessment.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02668-24","type":"journal-article","created":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T21:08:54Z","timestamp":1746047334000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["The impact of temperature and insect-specific viruses on the transmission of alphaviruses by\n                    <i>Aedes japonicus japonicus<\/i>"],"prefix":"10.1128","volume":"13","author":[{"given":"Stephanie","family":"Jansen","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01evwfd48","id-type":"ROR","asserted-by":"publisher"}],"name":"Bernhard Nocht Institute for Tropical Medicine","place":["Hamburg, Germany"]},{"name":"Faculty of Mathematics, Informatics and Natural Sciences, University of Hamburg","place":["Hamburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u00e1niel","family":"Cadar","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01evwfd48","id-type":"ROR","asserted-by":"publisher"}],"name":"Bernhard Nocht Institute for Tropical Medicine","place":["Hamburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana Christina","family":"Hey","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01evwfd48","id-type":"ROR","asserted-by":"publisher"}],"name":"Bernhard Nocht Institute for Tropical Medicine","place":["Hamburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Helms","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01evwfd48","id-type":"ROR","asserted-by":"publisher"}],"name":"Bernhard Nocht Institute for Tropical Medicine","place":["Hamburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Unchana","family":"Lange","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01evwfd48","id-type":"ROR","asserted-by":"publisher"}],"name":"Bernhard Nocht Institute for Tropical Medicine","place":["Hamburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bal\u00e1zs","family":"Horv\u00e1th","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01evwfd48","id-type":"ROR","asserted-by":"publisher"}],"name":"Bernhard Nocht Institute for Tropical Medicine","place":["Hamburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna","family":"J\u00f6st","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01evwfd48","id-type":"ROR","asserted-by":"publisher"}],"name":"Bernhard Nocht Institute for Tropical Medicine","place":["Hamburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolf-Peter","family":"Pfitzner","sequence":"additional","affiliation":[{"name":"Kommunale Aktionsgemeinschaft zur Bek\u00e4mpfung der Schnakenplage e. 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