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Fungal pathogens, especially\n                      <jats:italic toggle=\"yes\">Candida auris,<\/jats:italic>\n                      have emerged as clinically important pathogens involved in causing systemic infections and high mortality. Therefore, antimicrobial compounds effective against\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      are needed to tackle infections caused by these multidrug-resistant yeast pathogens. A soil bacterium was isolated from soil in Delhi, India, exhibited broad-spectrum antimicrobial activities against various pathogenic bacteria, such as\n                      <jats:italic toggle=\"yes\">Bacillus subtilis<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Staphylococcus aureus,<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Escherichia coli,<\/jats:italic>\n                      and pathogenic fungi, including\n                      <jats:italic toggle=\"yes\">Candida albicans, Fusarium oxysporum, Aspergillus fumigatus,<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Candida glabrata<\/jats:italic>\n                      . It was subjected to whole-genome sequencing and resulted in a high-quality draft genome with a single contig of 9.61 Mb genome size and 72.1% GC content. Phylogenetic analysis and average nucleotide identity identified that the strain belongs to\n                      <jats:italic toggle=\"yes\">Streptomyces chrestomyceticus<\/jats:italic>\n                      . The genome annotation predicted plant growth-promoting traits and stress-response genes. Genome mining revealed non-ribosomal peptide synthetase, polyketide synthase (PKS), and hybrid biosynthetic gene clusters (BGCs) synthesizing known and unknown metabolites. Most of the encoded compounds by the BGCs were reported for antibacterial and antitumor activities, and two BGCs were identified as antifungal clusters. A trans-acyltransferase polyketide synthase (transAT-PKS) cluster was identified with 27% and 19% similarity to cycloheximide and 9-methylstreptimidone clusters, respectively. The comparison of 9-methylstreptimidone and transAT-PKS cluster revealed differences in the organization of genes in closely related strains. The crude extract of\n                      <jats:italic toggle=\"yes\">S. chrestomyceticus<\/jats:italic>\n                      B5 possessed strong antibacterial and antifungal activities. The bioassay-guided purification and LC-MS identified an antifungal compound (streptimidone derivative or isomer, molecular mass 293.1 Da), which specifically inhibited\n                      <jats:italic toggle=\"yes\">C. glabrata<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      . The transAT-PKS cluster might be responsible for encoding this streptimidone derivative or isomer.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      The study highlighted the biosynthetic potential of\n                      <jats:italic toggle=\"yes\">Streptomyces chrestomyceticus<\/jats:italic>\n                      B5, which is a promising strain for producing various bioactive metabolites and is rich in biosynthetic gene clusters responsible for encoding multiple metabolites and producing an antifungal compound, a streptimidone derivative or isomer, active against drug-resistant\n                      <jats:italic toggle=\"yes\">Candida auris<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02688-25","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T13:00:09Z","timestamp":1774875609000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Genomic insights and biosynthetic gene cluster analysis of\n                    <i>Streptomyces chrestomyceticus<\/i>\n                    strain B5 showing antimicrobial activities"],"prefix":"10.1128","volume":"14","author":[{"given":"Aruna","family":"Kumari","sequence":"first","affiliation":[{"name":"Genomics and Genome Biology, CSIR-Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research (CSIR)","place":["New Delhi, India"]},{"id":[{"id":"https:\/\/ror.org\/053rcsq61","id-type":"ROR","asserted-by":"publisher"}],"name":"Academy of Scientific and Innovative Research (AcSIR)","place":["Ghaziabad, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonam","family":"Nain","sequence":"additional","affiliation":[{"name":"Genomics and Genome Biology, CSIR-Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research (CSIR)","place":["New Delhi, India"]},{"id":[{"id":"https:\/\/ror.org\/053rcsq61","id-type":"ROR","asserted-by":"publisher"}],"name":"Academy of Scientific and Innovative Research (AcSIR)","place":["Ghaziabad, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Praveen","family":"Singh","sequence":"additional","affiliation":[{"name":"Genomics and Genome Biology, CSIR-Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research (CSIR)","place":["New Delhi, India"]},{"id":[{"id":"https:\/\/ror.org\/053rcsq61","id-type":"ROR","asserted-by":"publisher"}],"name":"Academy of Scientific and Innovative Research (AcSIR)","place":["Ghaziabad, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nar Singh","family":"Chauhan","sequence":"additional","affiliation":[{"name":"Genomics and Genome Biology, CSIR-Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research (CSIR)","place":["New Delhi, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swarnendu","family":"Bag","sequence":"additional","affiliation":[{"name":"Genomics and Genome Biology, CSIR-Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research (CSIR)","place":["New Delhi, India"]},{"id":[{"id":"https:\/\/ror.org\/053rcsq61","id-type":"ROR","asserted-by":"publisher"}],"name":"Academy of Scientific and Innovative Research (AcSIR)","place":["Ghaziabad, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3320-4491","authenticated-orcid":true,"given":"Rakesh","family":"Sharma","sequence":"additional","affiliation":[{"name":"Genomics and Genome Biology, CSIR-Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research (CSIR)","place":["New Delhi, India"]},{"id":[{"id":"https:\/\/ror.org\/053rcsq61","id-type":"ROR","asserted-by":"publisher"}],"name":"Academy of Scientific and Innovative Research (AcSIR)","place":["Ghaziabad, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)02724-0"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-022-01112-0"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(23)00692-8"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(23)00114-3"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-019-0322-2"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3004404"},{"key":"e_1_3_4_8_2","volume-title":"Medical microbiology","author":"Dixon DM","year":"1996","unstructured":"Dixon DM, Walsh TJ. 1996. 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