{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T17:02:31Z","timestamp":1789405351176,"version":"build-2803163510"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T00:00:00Z","timestamp":1666742400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T00:00:00Z","timestamp":1666742400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"National Natural Science Foundation for Key Programs of China Grants","award":["82130065"],"award-info":[{"award-number":["82130065"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170201"],"award-info":[{"award-number":["32170201"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82002191"],"award-info":[{"award-number":["82002191"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7222014"],"award-info":[{"award-number":["7222014"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"name":"FENG foundation","award":["FFBR 202103"],"award-info":[{"award-number":["FFBR 202103"]}]},{"name":"Research Foundation of Capital Institute of Pediatrics","award":["CXYJ-2021-04"],"award-info":[{"award-number":["CXYJ-2021-04"]}]},{"name":"Beijing Medical Research Institute","award":["BMR2019-11"],"award-info":[{"award-number":["BMR2019-11"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2022,10,26]]},"abstract":"<jats:p>MPXV outbreaks rapidly grew in the first half of 2022, and this virus has been recognized as an increasing public health threat, particularly in the context of the COVID-19 pandemic. Thus, developing reliable and fast detection methods for MPXV is necessary.<\/jats:p>","DOI":"10.1128\/spectrum.02714-22","type":"journal-article","created":{"date-parts":[[2022,9,26]],"date-time":"2022-09-26T13:07:35Z","timestamp":1664197655000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":66,"title":["Development of a Loop-Mediated Isothermal Amplification Method for Rapid and Visual Detection of Monkeypox Virus"],"prefix":"10.1128","volume":"10","author":[{"given":"Junxia","family":"Feng","sequence":"first","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanhua","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohu","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanling","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinghua","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanqing","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Gan","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongtong","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziying","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuheng","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanyu","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyan","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6939-9676","authenticated-orcid":true,"given":"Jing","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Capital Institute of Pediatrics, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1959.tb00328.x"},{"key":"e_1_3_3_3_2","first-page":"593","article-title":"A human infection caused by monkeypox virus in Basankusu Territory, Democratic Republic of the Congo","volume":"46","author":"Ladnyj ID","year":"1972","unstructured":"Ladnyj ID, Ziegler P, Kima E. 1972. A human infection caused by monkeypox virus in Basankusu Territory, Democratic Republic of the Congo. Bull World Health Organ 46:593\u2013597.","journal-title":"Bull World Health Organ"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.81215-0"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/d41586-022-01421-8"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/d41586-022-01587-1"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/20002297.2018.1510712"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01101"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.13586"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006381"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.1.257-263.2004"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.11150\/kansenshogakuzasshi.84.431"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2019.113775"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-021-03178-0"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.21037\/apm-21-1387"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2011.04.002"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-5-2"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.4265\/bio.22.97"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiaa559"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2006.03.012"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2010.07.012"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2016.07.024"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-022-00611-6"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21494"},{"key":"e_1_3_3_26_2","doi-asserted-by":"crossref","unstructured":"Yuan J Liu W Huang L. 2014. A method used to prevent nucleic acid contamination and to indicate the result of nucleic acid thermostatic amplification reaction. China patent CN 201210371448.5.","DOI":"10.1038\/srep12723"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.02714-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.02714-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T13:48:41Z","timestamp":1666792121000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.02714-22"}},"subtitle":[],"editor":[{"given":"William","family":"Lainhart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,10,26]]},"references-count":25,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,10,26]]}},"alternative-id":["10.1128\/spectrum.02714-22"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.02714-22","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,26]]},"assertion":[{"value":"2022-07-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02714-22"}}