{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:16:02Z","timestamp":1790381762786,"version":"4.1.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100022110","name":"BioM\u00e9rieux","doi-asserted-by":"crossref","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100022110","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,10,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              The performance of a novel selective agar was evaluated against the performance of conventional mycobacterial cultures, i.e., a combination of the mycobacterial growth indicator tube (MGIT) with L\u00f6wenstein-Jensen (LJ), for the detection of nontuberculous mycobacteria (NTM) in sputum samples from people with cystic fibrosis (pwCF). Two hundred eighty-three sputum samples (231 fresh sputum and 52 spiked sputum) from 143 pwCF were collected. They were inoculated without prior decontamination on NTM Elite agar (30\u00b0C \u00b1 2\u00b0C for 28 days) and inoculated on both MGIT and LJ (35\u00b0C\u201337\u00b0C for 6\u20138 weeks) after N-acetyl-L-cysteine-2% sodium hydroxide decontamination. NTM were identified by Matrix-Assisted Laser Desorption Ionization\/Time of Flight Mass Spectrometry and\/or PCR, and whole-genome sequencing. A total of 67 NTM were recovered overall by the combination of all culture media. NTM Elite agar allowed the recovery of 65 NTM (97%), compared to 22 for the conventional MGIT and LJ media combination (32.8%), including 22 NTM for MGIT (32.8%) and 3 NTM with the LJ medium (4.5%). For\n              <jats:italic>Mycobacterium abscessus<\/jats:italic>\n              complex, the sensitivity of NTM Elite agar was 95% compared with a sensitivity of 30% for the conventional MGIT and LJ media combination. Overall, 17.3% of cultures on NTM Elite agar were contaminated with other micro-organisms vs 46.3% on MGIT and 77% on LJ. This study shows that the novel selective agar (NTM Elite agar) significantly outperforms the conventional MGIT and LJ media combination in terms of sensitivity, selectivity, and ease of culture, without the requirement of an L3 laboratory.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                Nontuberculous mycobacteria (NTM) are significant pulmonary pathogens in patients with pre-existing structural lung conditions such as cystic fibrosis, bronchiectasis, or chronic obstructive pulmonary disease.\n                <jats:italic>Mycobacterium avium<\/jats:italic>\n                complex and\n                <jats:italic>Mycobacterium abscessus<\/jats:italic>\n                complex (MABSC) are the most frequently isolated organisms. Compared to the recommended culture method for NTM, which combines solid and liquid culture media, NTM Elite agar enables a faster\/easier diagnosis and speeds up identification and susceptibility testing as the final reading is at 28 days instead of 6\u20138 weeks for the conventional mycobacterial cultures. In addition, for the NTM Elite agar, no decontamination stage before inoculation is necessary, unlike the conventional mycobacterial cultures. NTM Elite agar is derived from a formulation of medium adapted to rapidly growing mycobacteria (RGM). The medium enables the growth of RGM while suppressing other flora. It is supported with published clinical data showing the benefits of this medium.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.02736-23","type":"journal-article","created":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T13:03:50Z","timestamp":1724850230000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Multicenter study of the performance of NTM Elite agar for the detection of nontuberculous mycobacteria from patients with cystic fibrosis"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8321-3770","authenticated-orcid":false,"given":"Emmanuel","family":"Andr\u00e9","sequence":"first","affiliation":[{"name":"Laboratory Medicine Department, UZ Leuven University Hospitals, Leuven, Belgium"},{"name":"Department of Microbiology, Immunology, and Transplantation, Laboratory of Clinical Microbiology, KU Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie","family":"Lorent","sequence":"additional","affiliation":[{"name":"Respiratory Diseases Department, University Hospitals Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kurt","family":"Beuselinck","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Department, UZ Leuven University Hospitals, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Deiwick","sequence":"additional","affiliation":[{"name":"University Hospital M\u00fcnster, Institute of Medical Microbiology, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lieven","family":"Dupont","sequence":"additional","affiliation":[{"name":"Respiratory Diseases Department, University Hospitals Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1658-6883","authenticated-orcid":false,"given":"Johanne","family":"Gafsi","sequence":"additional","affiliation":[{"name":"BioM\u00e9rieux Global Clinical Affairs, Marcy-l\u2019Etoile, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lies","family":"Laenen","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Department, UZ Leuven University Hospitals, Leuven, Belgium"},{"name":"Department of Microbiology, Immunology, and Transplantation, Laboratory of Clinical Microbiology, KU Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lise","family":"Raymaekers","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Department, UZ Leuven University Hospitals, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Van Bleyenbergh","sequence":"additional","affiliation":[{"name":"Respiratory Diseases Department, University Hospitals Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7225-7508","authenticated-orcid":false,"given":"John D.","family":"Perry","sequence":"additional","affiliation":[{"name":"Microbiology Department, Freeman Hospital, Newcastle upon Tyne, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5155-3383","authenticated-orcid":false,"given":"Barbara C.","family":"Kahl","sequence":"additional","affiliation":[{"name":"University Hospital M\u00fcnster, Institute of Medical Microbiology, M\u00fcnster, 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Tools - Belgian Classification for Micro-Organisms Based on Their Biological Risks. Available from: https:\/\/www.biosafety.be\/content\/tools-belgian-classification-micro-organisms-based-their-biological-risks#lists. Retrieved 22 Dec 2022."},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1710-1711.2003"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jctube.2018.07.003"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2019.e02684"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa241"},{"key":"e_1_3_3_27_2","volume-title":"M48 laboratory detection and identification of mycobacteria","author":"CLSI","year":"2018","unstructured":"CLSI. 2018. 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