{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T01:15:51Z","timestamp":1788225351680,"version":"build-2803163510"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,7,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Vibrio cholerae<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">V. cholerae<\/jats:italic>\n                      ), causing deadly cholera, which is responsible for significant public health problems in endemic regions, should be monitored in contaminated environments. Here, we report an integrated protocol incorporating alkaline lysis DNA extraction and loop-mediated isothermal amplification (LAMP) targeting the\n                      <jats:italic toggle=\"yes\">V. cholerae rpo<\/jats:italic>\n                      B gene and producing a colorimetric reading within 1 h. This protocol yielded 100% specificity for\n                      <jats:italic toggle=\"yes\">V. cholerae<\/jats:italic>\n                      after testing three non-cholerae\n                      <jats:italic toggle=\"yes\">Vibrio<\/jats:italic>\n                      species and four\n                      <jats:italic toggle=\"yes\">Enterobacteriaceae<\/jats:italic>\n                      as negative controls. A positive signal was detected across a range of 3 \u00d7 10\n                      <jats:sup>8<\/jats:sup>\n                      to 3 \u00d7 10\n                      <jats:sup>3<\/jats:sup>\n                      cells\/mL for four\n                      <jats:italic toggle=\"yes\">V. cholerae<\/jats:italic>\n                      isolates within 30\u201360 min. Following the inoculation of 3 \u00d7 10\n                      <jats:sup>4<\/jats:sup>\n                      <jats:italic toggle=\"yes\">V. cholerae<\/jats:italic>\n                      cells into five simulated environmental matrices (soil, sand, seawater, tap water, and anthropogenic effluents), positive results were similarly obtained between 3 \u00d7 10\n                      <jats:sup>4<\/jats:sup>\n                      and 3 \u00d7 10\n                      <jats:sup>3<\/jats:sup>\n                      cells\/mL over the same time interval. In contrast, at the same conditions,\n                      <jats:italic toggle=\"yes\">Vibrio alginolyticus<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">V. alginolyticus<\/jats:italic>\n                      ) and\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      mock-infected matrices, which were used as negative controls, remained negative. These data suggested that this LAMP protocol could be implemented in the field to monitor\n                      <jats:italic toggle=\"yes\">V. cholerae<\/jats:italic>\n                      in environmental matrices at the point of interest in countries affected by this deadly pathogen.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Cholera is an intestinal infection caused by the bacterium\n                        <jats:italic toggle=\"yes\">Vibrio cholerae<\/jats:italic>\n                        , resulting in vomiting and acute watery diarrhea. If left untreated, it can cause severe dehydration and death. Although various detection tests have been developed for different types of samples, cholera remains endemic in several countries, particularly where access to safe drinking water is limited and sanitation infrastructure remains inadequate. The objective of our research is to explore different environmental matrices to design a screening system for\n                        <jats:italic toggle=\"yes\">V. cholerae<\/jats:italic>\n                        in environmental samples, thus contributing to the development of a system that can be used in the environment.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02753-25","type":"journal-article","created":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T13:01:14Z","timestamp":1779368474000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Isothermal amplification and colorimetric detection of\n                    <i>Vibrio cholerae<\/i>\n                    in environmental matrices"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-0584-6449","authenticated-orcid":false,"given":"M.","family":"Adolphe","sequence":"first","affiliation":[{"name":"Aix-Marseille Universit\u00e9, MEPHI","place":["Marseille, France"]},{"id":[{"id":"https:\/\/ror.org\/0068ff141","id-type":"ROR","asserted-by":"publisher"}],"name":"IHU M\u00e9diterran\u00e9e Infection","place":["Marseille, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Boualam","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9, MEPHI","place":["Marseille, France"]},{"id":[{"id":"https:\/\/ror.org\/0068ff141","id-type":"ROR","asserted-by":"publisher"}],"name":"IHU M\u00e9diterran\u00e9e Infection","place":["Marseille, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8575-4500","authenticated-orcid":true,"given":"H.","family":"Oumarou Hama","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9, MEPHI","place":["Marseille, France"]},{"id":[{"id":"https:\/\/ror.org\/0068ff141","id-type":"ROR","asserted-by":"publisher"}],"name":"IHU M\u00e9diterran\u00e9e Infection","place":["Marseille, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5788-552X","authenticated-orcid":false,"given":"E.","family":"Terrer","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9, MEPHI","place":["Marseille, France"]},{"id":[{"id":"https:\/\/ror.org\/0068ff141","id-type":"ROR","asserted-by":"publisher"}],"name":"IHU M\u00e9diterran\u00e9e Infection","place":["Marseille, France"]},{"name":"Assistance Publique des H\u00f4pitaux de Marseille, APHM","place":["Marseille, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-018-0005-8"},{"key":"e_1_3_4_3_2","first-page":"105","volume-title":"L\u2019Africanisation du chol\u00e9ra","author":"R\u00e9my G","year":"1988","unstructured":"R\u00e9my G, Avril-juin Dh. 1988. 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