{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T15:25:22Z","timestamp":1767713122473,"version":"3.41.2"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T00:00:00Z","timestamp":1702339200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T00:00:00Z","timestamp":1702339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["NSFC 81973372, 21920102003, 82061128001"],"award-info":[{"award-number":["NSFC 81973372, 21920102003, 82061128001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2023,12,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The polymorphism at amino acid 17 of quinolone resistance-determining region of GyrA has been stated with a potential role in fluoroquinolone susceptibility in different mycobacterial species. However, no study has provided dependable evidence so far. Here, we verified that gene-edited\n            <jats:italic>Mycobacterium abscessus<\/jats:italic>\n            mutants bearing Ser\/Gly at this position were more susceptible to fluoroquinolones than their parent strain and the revertant that supports mycobacteria containing Ser\/Gly at this position were more susceptible to fluoroquinolones than those containing Ala.\n          <\/jats:p>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Fluoroquinolones (FQs) play a key role in the treatment regimens against tuberculosis and non-tuberculous mycobacterial infections. However, there are significant differences in the sensitivities of different mycobacteria to FQs. In this study, we proved that this is associated with the polymorphism at amino acid 17 of quinolone resistance-determining region of Gyrase A by gene editing. This is the first study using CRISPR-associated recombination for gene editing in\n              <jats:italic>Mycobacterium abscessus<\/jats:italic>\n              to underscore the contribution of the amino acid substitutions in GyrA to FQ susceptibilities in mycobacteria.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.02809-23","type":"journal-article","created":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T15:32:59Z","timestamp":1697211179000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Amino acid 17 in QRDR of Gyrase A plays a key role in fluoroquinolones susceptibility in mycobacteria"],"prefix":"10.1128","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0893-2534","authenticated-orcid":false,"given":"Shuai","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences , Guangzhou, China"},{"name":"University of Chinese Academy of Sciences , Beijing, China"},{"name":"Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences , Guangzhou, China"},{"name":"China-New Zealand Joint Laboratory on Biomedicine and Health , Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9701-4289","authenticated-orcid":false,"given":"Jingran","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences , Guangzhou, China"},{"name":"Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences , Guangzhou, China"},{"name":"China-New Zealand Joint Laboratory on Biomedicine and Health , Guangzhou, China"},{"name":"School of Life Sciences, University of Science and Technology of China , Hefei, Anhui, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1993-9547","authenticated-orcid":false,"given":"H. M. Adnan","family":"Hameed","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences , Guangzhou, China"},{"name":"University of Chinese Academy of Sciences , Beijing, China"},{"name":"Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences , Guangzhou, China"},{"name":"China-New Zealand Joint Laboratory on Biomedicine and Health , Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Ding","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences , Guangzhou, China"},{"name":"Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences , Guangzhou, China"},{"name":"China-New Zealand Joint Laboratory on Biomedicine and Health , Guangzhou, China"},{"name":"Institutes of Physical Science and Information Technology, Anhui University , Hefei, Anhui, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Guan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease, Guangzhou Chest Hospital , Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiange","family":"Fang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences , Guangzhou, China"},{"name":"University of Chinese Academy of Sciences , Beijing, China"},{"name":"Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences , Guangzhou, China"},{"name":"China-New Zealand Joint Laboratory on Biomedicine and Health , Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiacong","family":"Peng","sequence":"additional","affiliation":[{"name":"Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences , Guangzhou, China"},{"name":"State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, The National Center for Respiratory Medicine, The First Affiliated Hospital of Guangzhou Medical University , Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Biyi","family":"Su","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease, Guangzhou Chest Hospital , Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shangming","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease, Guangzhou Chest Hospital , Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaoju","family":"Tan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease, Guangzhou Chest Hospital , Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory","family":"M. Cook","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Biomedical Sciences, University of Otago , Dunedin, New Zealand"},{"name":"Maurice Wilkins Centre for Molecular Biodiscovery, The University of Auckland, Private Bag , Auckland, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoliang","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Infectious Diseases, Guangdong Provincial Clinical Research Center for Tuberculosis, Shenzhen Third People's Hospital , Shenzhen, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongping","family":"Lin","sequence":"additional","affiliation":[{"name":"Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences , Guangzhou, China"},{"name":"State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, The National Center for Respiratory Medicine, The First Affiliated Hospital of Guangzhou Medical University , Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nanshan","family":"Zhong","sequence":"additional","affiliation":[{"name":"Guangdong-Hong Kong-Macao Joint Laboratory of Respiratory Infectious Diseases, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences , Guangzhou, China"},{"name":"State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, The National Center for Respiratory Medicine, The First Affiliated Hospital of Guangzhou Medical University , Guangzhou, China"},{"name":"Department of Microbiology and Immunology, School of Biomedical Sciences, University of Otago , Dunedin, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinxing","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key 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