{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:21:54Z","timestamp":1785424914710,"version":"3.56.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["23K07756"],"award-info":[{"award-number":["23K07756"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","award":["22HA2001"],"award-info":[{"award-number":["22HA2001"]}],"id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","award":["25HA2001"],"award-info":[{"award-number":["25HA2001"]}],"id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["24fk0108673"],"award-info":[{"award-number":["24fk0108673"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Mycobacterium marinum<\/jats:italic>\n                      is the etiological agent of cutaneous nontuberculous mycobacteriosis, inducing granuloma formation in human skin. Other mycobacterial species, including\n                      <jats:italic toggle=\"yes\">Mycobacterium ulcerans<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Mycobacterium chelonae<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">Mycobacterium fortuitum<\/jats:italic>\n                      , also cause cutaneous infections, with\n                      <jats:italic toggle=\"yes\">M. ulcerans<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">M. ulcerans<\/jats:italic>\n                      subsp.\n                      <jats:italic toggle=\"yes\">shinshuense<\/jats:italic>\n                      showing close genetic relatedness to\n                      <jats:italic toggle=\"yes\">M. marinum. M. ulcerans<\/jats:italic>\n                      is a recognized causative agent of Buruli ulcer, and numerous molecular detection and diagnostic methods have been established. However, there is a lack of specific detection methods for\n                      <jats:italic toggle=\"yes\">M. marinum<\/jats:italic>\n                      . Currently, confirming\n                      <jats:italic toggle=\"yes\">M. marinum<\/jats:italic>\n                      infection requires 16S rRNA or housekeeping gene sequencing, which is labor-intensive and time-consuming. In this study, we developed a one-step colorimetric loop-mediated isothermal amplification assay for simple and specific detection of\n                      <jats:italic toggle=\"yes\">M. marinum<\/jats:italic>\n                      . The results were confirmed by visual inspection of color change in the reaction solution, eliminating the need for specialized equipment. Our proposed workflow can be completed within 60 minutes from sample preparation to detection with high sensitivity. To the best of our knowledge, the loop-mediated isothermal amplification assay developed in this study represents the only test capable of specifically detecting\n                      <jats:italic toggle=\"yes\">M. marinum<\/jats:italic>\n                      utilizing only the loop-mediated isothermal amplification reaction. Consequently, this loop-mediated isothermal amplification assay has the potential for implementation in routine diagnosis of\n                      <jats:italic toggle=\"yes\">M. marinum<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Mycobacterium marinum<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">Mycobacterium ulcerans<\/jats:italic>\n                      , which are frequently reported as etiological agents of cutaneous nontuberculous mycobacteria, exhibit similar characteristics in terms of symptomatology and patient demographics. However, there is currently no simple identification method to distinguish between these two species. The one-step loop-mediated isothermal amplification method developed in this study represents a rapid diagnostic approach that yields results in a short time frame and does not necessitate specialized precision instruments, as the results can be visually determined. Consequently, the loop-mediated isothermal amplification method established in this study is anticipated to contribute significantly to the advancement of diagnostic techniques for cutaneous nontuberculous mycobacteria.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.02906-24","type":"journal-article","created":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T08:39:26Z","timestamp":1749631166000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["One-step loop-mediated isothermal amplification system for\n                    <i>Mycobacterium marinum<\/i>\n                    detection"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5667-0191","authenticated-orcid":true,"given":"Kayo","family":"Okumura","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/001ggbx22","id-type":"ROR","asserted-by":"publisher"}],"name":"Research Center for Biosafety, Laboratory Animal and Pathogen Bank, National Institute of Infectious Diseases, Japan Institute for Health Security, Aoba-cho","place":["Higashimurayama, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Miyamoto","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/001ggbx22","id-type":"ROR","asserted-by":"publisher"}],"name":"Leprosy Research Center, National Institute of Infectious Diseases, Japan Institute for Health Security, Aoba-cho","place":["Higashimurayama, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2288-077X","authenticated-orcid":true,"given":"Satoshi","family":"Mitarai","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/012daep68","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, Japan Anti-tuberculosis Association","place":["Kiyose, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manabu","family":"Ato","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/001ggbx22","id-type":"ROR","asserted-by":"publisher"}],"name":"Leprosy Research Center, National Institute of Infectious Diseases, Japan Institute for Health Security, Aoba-cho","place":["Higashimurayama, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/39.4.315"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.2018.1447171"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.tnmi7-0038-2016"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.2105\/ajph.51.6.883"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ937"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268817001066"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268811001798"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00069-18"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5403.854"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0305877101"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2019.105671"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2008.01264.x"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01041-07"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.2144\/000113072"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S404929"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-12-8"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12030629"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.11.3133-3136.2000"},{"key":"e_1_3_4_20_2","unstructured":"World Health Organization. 2012. 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