{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:33:12Z","timestamp":1791253992805,"version":"4.1.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T00:00:00Z","timestamp":1676332800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T00:00:00Z","timestamp":1676332800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100009592","name":"Beijing Municipal Science and Technology Commission","doi-asserted-by":"publisher","award":["Z211100002921004"],"award-info":[{"award-number":["Z211100002921004"]}],"id":[{"id":"10.13039\/501100009592","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007452","name":"Ministry of Health of China | Wu Jieping Medical Foundation","doi-asserted-by":"publisher","award":["320.6750.2022-08-1"],"award-info":[{"award-number":["320.6750.2022-08-1"]}],"id":[{"id":"10.13039\/100007452","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010270","name":"BMHB | Capital Health Research and Development of Special Fund","doi-asserted-by":"publisher","award":["2022-2-2183"],"award-info":[{"award-number":["2022-2-2183"]}],"id":[{"id":"10.13039\/501100010270","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2023,2,14]]},"abstract":"<jats:p>This study established and evaluated a complete metagenomics next-generation sequencing assay to improve the diagnosis of suspected ascitic infection in a clinical laboratory affiliated with a hospital. The assay is superior to traditional culture testing and will aid in the early and accurate identification of pathogens, particularly in patients with polymicrobial infections.<\/jats:p>","DOI":"10.1128\/spectrum.02946-22","type":"journal-article","created":{"date-parts":[[2023,1,10]],"date-time":"2023-01-10T14:05:11Z","timestamp":1673359511000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Clinical Evaluation of Metagenomic Next-Generation Sequencing Method for the Diagnosis of Suspected Ascitic Infection in Patients with Liver Cirrhosis in a Clinical Laboratory"],"prefix":"10.1128","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7685-5924","authenticated-orcid":true,"given":"Hao-Xin","family":"Wu","sequence":"first","affiliation":[{"name":"Beijing YouAn Hospital, Capital Medical University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei-Li","family":"Wei","sequence":"additional","affiliation":[{"name":"Beijing Institute of Hepatology, Beijing YouAn Hospital, Capital Medical University, Beijing Precision Medicine and Transformation Engineering Technology Research Center of Hepatitis and Liver Cancer, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Beijing YouAn Hospital, Capital Medical University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Han","sequence":"additional","affiliation":[{"name":"Beijing YouAn Hospital, Capital Medical University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Guo","sequence":"additional","affiliation":[{"name":"Beijing Institute of Hepatology, Beijing YouAn Hospital, Capital Medical University, Beijing Precision Medicine and Transformation Engineering Technology Research Center of Hepatitis and Liver Cancer, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing YouAn Hospital, Capital Medical University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De-Xi","family":"Chen","sequence":"additional","affiliation":[{"name":"Beijing Institute of Hepatology, Beijing YouAn Hospital, Capital Medical University, Beijing Precision Medicine and Transformation Engineering Technology Research Center of Hepatitis and Liver Cancer, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Hou","sequence":"additional","affiliation":[{"name":"Beijing YouAn Hospital, Capital Medical University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1521-8294","authenticated-orcid":true,"given":"Zhong-Jie","family":"Hu","sequence":"additional","affiliation":[{"name":"Beijing YouAn Hospital, Capital Medical University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-1105-z"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.238170.118"},{"key":"e_1_3_3_4_2","unstructured":"FDA. FDA laboratory developed tests. https:\/\/www.fda.gov\/medical-devices\/vitro-diagnostics\/laboratory-developed-tests. Accessed on 10\/5\/18."},{"key":"e_1_3_3_5_2","first-page":"100","article-title":"Current status and challenges of pathogen metagenomic high-throughput sequencing testing implemented in a clinical laboratory affiliated to a hospital","volume":"2","author":"Dongsheng H","year":"2022","unstructured":"Dongsheng H, Xiaoling M, Wenjuan W. 2022. Current status and challenges of pathogen metagenomic high-throughput sequencing testing implemented in a clinical laboratory affiliated to a hospital. Chinese J Laboratory Medicine 2:100\u2013104.","journal-title":"Chinese J Laboratory Medicine"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2020-321790"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.03.024"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/hvab061"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01143-21"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.741220"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2014.07.005"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/liv.15083"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12072-018-09923-2"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.31884"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2018.9159"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.3343\/alm.2020.40.1.63"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22564"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00420"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/liv.13929"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.894678"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-016-0873-3"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2019.09.015"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-021-00520-7"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00013-11"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.641202"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-022-04116-8"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-018-0349-6"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2012-0651-OA"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1097\/MEG.0000000000000927"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1923"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.876495"},{"key":"e_1_3_3_32_2","first-page":"3780","article-title":"A unified view of multi-label performance measures","volume":"70","author":"Wu X","year":"2017","unstructured":"Wu X, Zhou Z. 2017. A unified view of multi-label performance measures. PMLR 70:3780\u20133788.","journal-title":"PMLR"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.02946-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.02946-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T15:00:07Z","timestamp":1676386807000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.02946-22"}},"subtitle":[],"editor":[{"given":"Vittal","family":"Ponraj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,2,14]]},"references-count":31,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,2,14]]}},"alternative-id":["10.1128\/spectrum.02946-22"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.02946-22","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,14]]},"assertion":[{"value":"2022-07-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02946-22"}}